Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,190,817	G→A	C184C (TGC→TGT)	U712_10975 ←	Tryptophan synthase beta chain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,190,817	0	G	A	100.0%	234.9 / NA	75	C184C (TGC→TGT)	U712_10975	Tryptophan synthase beta chain
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/32);  total (43/32)

TATCAGGCAGAGTGCCTTCAATGTGTTTCAGCTGATCCTTCGCTTCCTCTCCAATCATTTTCTGAAATTCTCGGACCACCTGCGGATAAGGGTGCGGTCCGACAACTGATCCAATCATGTAAAAATGATCCTCGCAATGTTGAACCCAATACCGAATCGCCTCATTTGTGGCATCCTTCAATGTGCCATTTCCGCTTGTTACAGGAACAACTTCCGCCCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCT  >  CP006881/2190684‑2190950                                                                                                                                      |                                                                                                                                      tATCAGGCATAGTGCCTTCAATACGTTTCAACTGATCCTTCGCTTCCTCTCCGATCATTTTTTTAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACACCTGATCCAATCATATAAAAGTGATCCTCACAATg                                                                                                                                  >  3:255024/1‑139 (MQ=255)  aTCAGGCATAGTGCCTTCAATACGTTTCAACTGATCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATg                                                                                                                                  <  1:43208/138‑1 (MQ=255)   tCAGGCATAGTGCCTTCAATACGTTTCAACTGATCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGCTAAGGATGCGGGCCGACAACTGCTCCAATCATATAAAAGTGATCCTCACAATgct                                                                                                                                >  6:27009/1‑137 (MQ=255)     aGGCATAGTGCCTTCAATACGTTTCAACTGATCCTTCGCTTACTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTg                                                                                                                               <  3:370891/138‑1 (MQ=255)      ggCATAGTGCCTTCAATACGTTTCAACTGATCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGaa                                                                                                                             <  1:209052/139‑1 (MQ=255)        catagTGCCTTCAATACGTTTCAACTGATCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAAcc                                                                                                                           <  4:58103/136‑1 (MQ=255)        catagTGCCTTCAATACGTTTCAACTGATCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAAcc                                                                                                                           <  2:67587/136‑1 (MQ=255)             tGCCTTCAATACGTTTCAACTGATCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATgct                                                                                                                                <  1:30796/129‑3 (MQ=255)             tGCCTTCAATACGTTTCAACTGATCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATgct                                                                                                                                >  2:30796/1‑127 (MQ=255)                 ttCAATACGTTTCAACTGATCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCg                                                                                                                   >  1:25028/1‑138 (MQ=255)                    aatacgtTTCAACTGATCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGAt                                                                                                                <  3:177137/133‑1 (MQ=255)                      tacgtTTCAACTGATCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGc                                                                                                             <  8:38618/136‑1 (MQ=255)                      tacgtTTCAACTGATCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGc                                                                                                             <  2:85962/136‑1 (MQ=255)                           ttCAACTGATCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCAt                                                                                                        >  5:300311/1‑139 (MQ=255)                                cTGATCCTTCGCTTCCTCTACGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTtgtg                                                                                                   <  4:1893/139‑1 (MQ=255)                                   aTCCTTCGCTTCCTCTCCGATCGTTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGc                                                                                                 <  8:359594/138‑1 (MQ=255)                                   aTCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGCCCACTTGCGGATAAGGATGCGGGCCGCCAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTgg                                                                                                  >  4:269121/1‑137 (MQ=255)                                   aTCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGCTCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCa                                                                                                >  7:375362/1‑139 (MQ=255)                                   aTCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCGTTTGTGGCa                                                                                                <  8:67317/139‑1 (MQ=255)                                   aTCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCa                                                                                                <  2:158267/139‑1 (MQ=255)                                   aTCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCa                                                                                                >  4:255577/1‑139 (MQ=255)                                   aTCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCa                                                                                                <  1:266115/139‑1 (MQ=255)                                   aTCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCa                                                                                                <  3:255577/139‑1 (MQ=255)                                   aTCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCa                                                                                                <  2:439377/139‑1 (MQ=255)                                     ccTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATc                                                                                              >  7:405509/1‑139 (MQ=255)                                               ctctCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAAtgtt                                                                                    >  3:180842/1‑138 (MQ=255)                                                      aTCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGc                                                                                                             <  6:146598/107‑1 (MQ=255)                                                      aTCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGc                                                                                                             >  5:146598/1‑107 (MQ=255)                                                      aTCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTc                                                                             >  5:394300/1‑139 (MQ=255)                                                      aTCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTc                                                                             >  2:417067/1‑139 (MQ=255)                                                          ttttttGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCt                                                                         <  1:417067/139‑1 (MQ=255)                                                            ttttGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGc                                                                                                             <  7:275826/98‑1 (MQ=255)                                                            ttttGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGc                                                                                                             >  8:275826/4‑101 (MQ=255)                                                                gAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTa                                                                    <  1:58541/138‑1 (MQ=255)                                                                 aaaTTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACa                                                                  <  4:255024/139‑1 (MQ=255)                                                                       acGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGcac                                                             <  6:337274/137‑1 (MQ=255)                                                                           accactTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGcactacct                                                        >  2:21410/1‑137 (MQ=255)                                                                                          ggatGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACa                                                                  >  4:176492/4‑114 (MQ=255)                                                                                          ggatGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACa                                                                  <  3:176492/111‑1 (MQ=255)                                                                                          ggatGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCt                                         >  1:39586/4‑139 (MQ=255)                                                                                          ggatGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCt                                         >  2:103245/4‑139 (MQ=255)                                                                                              gCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCAt                                     >  3:427713/1‑139 (MQ=255)                                                                                               cGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGaa                                                                                                                             >  7:408300/1‑50 (MQ=255)                                                                                               cGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGaa                                                                                                                             <  8:408300/50‑1 (MQ=255)                                                                                                gggccGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGc                                   >  7:22630/4‑139 (MQ=255)                                                                                                     gACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGaaa                              <  6:300311/139‑1 (MQ=255)                                                                                                     gACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGaa                               >  2:5265/1‑138 (MQ=255)                                                                                                      aCAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCaag                                              >  4:237003/1‑122 (MQ=255)                                                                                                      aCAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCaag                                              <  3:237003/122‑1 (MQ=255)                                                                                                            gATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAgg                                                                >  1:222055/1‑98 (MQ=255)                                                                                                            gATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAgg                                                                <  2:222055/98‑1 (MQ=255)                                                                                                             aTCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACATCCTCCGCTCCACGAAGCTTCATGCGGAAAACGtt                         >  2:160209/1‑136 (MQ=255)                                                                                                                  aTCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACt                 >  5:256034/1‑139 (MQ=255)                                                                                                                     atatAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGGTACAGGCCCTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGCCTGGc              >  6:280550/4‑139 (MQ=255)                                                                                                                       atAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCgg            >  8:385208/2‑139 (MQ=255)                                                                                                                       atAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCgg            >  1:432761/2‑139 (MQ=255)                                                                                                                            agtGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAAc       <  2:25028/137‑1 (MQ=255)                                                                                                                              tGATCCTCACAATGCTGAACCCAGGACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACAt     <  6:256034/139‑1 (MQ=255)                                                                                                                               gATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCCTCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCTGAATACGTTCAGAGACTGGCGGGCAGCAtc    >  7:40735/1‑139 (MQ=255)                                                                                                                                aTCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCg                                                     <  4:401162/89‑1 (MQ=255)                                                                                                                                aTCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCg                                                     <  4:394679/89‑1 (MQ=255)                                                                                                                                aTCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCg                                                     >  3:401162/1‑89 (MQ=255)                                                                                                                                aTCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCg                                                     >  3:394679/1‑89 (MQ=255)                                                                                                                                aTCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAAc       <  6:31170/135‑1 (MQ=255)                                                                                                                                aTCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAAc       >  5:31170/1‑135 (MQ=255)                                                                                                                                aTCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACAtcc   >  8:70659/1‑139 (MQ=255)                                                                                                                                aTCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACAtcc   >  6:8528/1‑139 (MQ=255)                                                                                                                                aTCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACAtcc   >  6:82586/1‑139 (MQ=255)                                                                                                                                aTCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACAtcc   >  6:229740/1‑139 (MQ=255)                                                                                                                                aTCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACAtcc   >  5:220467/1‑139 (MQ=255)                                                                                                                                aTCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACAtcc   >  1:53647/1‑139 (MQ=255)                                                                                                                                aTCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACAgcc   >  5:336105/1‑139 (MQ=255)                                                                                                                                aTCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCAAGAGGAAAACGTTCAGAGACTGGAGGGCAACAtcc   >  6:353773/1‑139 (MQ=255)                                                                                                                                aTCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACATCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACAtcc   >  1:215560/1‑139 (MQ=255)                                                                                                                                 tCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACAtcct  >  5:447649/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                      TATCAGGCAGAGTGCCTTCAATGTGTTTCAGCTGATCCTTCGCTTCCTCTCCAATCATTTTCTGAAATTCTCGGACCACCTGCGGATAAGGGTGCGGTCCGACAACTGATCCAATCATGTAAAAATGATCCTCGCAATGTTGAACCCAATACCGAATCGCCTCATTTGTGGCATCCTTCAATGTGCCATTTCCGCTTGTTACAGGAACAACTTCCGCCCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCT  >  CP006881/2190684‑2190950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑