Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,173,382 T→C R122R (AGA→AGG)  U712_10870 ← Bifunctional protein BirA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,173,3820TC100.0% 130.3 / NA 41R122R (AGA→AGG) U712_10870Bifunctional protein BirA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/18);  total (23/18)

CCTGCACAACAGCTACTGCAGCAAGTAATGTGAGCTGCGGTGTTTTTTGAAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAATGCCATTTCCCTCTTGAGAATGCCATACCCTAGACATTCGACCTCTGCCGGCTGTTTGTTTGTCAGCCACCACAAGAGTGCCTTCCGGTGCGTTATTATTCGCAAGCTCATGCGCCGTTTTTTGCGTGCTTGAAATAACGTCTTGATAAATAAGATGCTGGCCCATTACTTCCGTT  >  CP006881/2173252‑2173514
                                                                                                                                  |                                                                                                                                    
ccTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATTCCATACCCTAGACATTCGGCCCCTTCCGGc                                                                                                                              >  1:58054/1‑139 (MQ=255)
  tGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCCAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATTCCATACCCTAGACATTCGGCCCCTGCCCGcgg                                                                                                                            >  6:9032/1‑137 (MQ=255)
  tGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCCTACAAACACCGTTTCCTTCTTGAGCATGCCATACCCTAGCCATTAGGCCCCGTCCGGCtg                                                                                                                            >  4:146728/1‑139 (MQ=255)
  tGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCtg                                                                                                                            >  7:50631/1‑139 (MQ=255)
  tGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCtg                                                                                                                            >  7:44210/1‑139 (MQ=255)
  tGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCGTCCGGcgg                                                                                                                            >  1:106015/1‑137 (MQ=255)
  tGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATAAGGCTCATCCAAACCCCGTTTCCTTGTTGAGAATGCCATACCCGAGACATTCGGCCCCGTCCGGcgg                                                                                                                            >  1:370288/1‑137 (MQ=255)
    cacaACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCtgtt                                                                                                                          >  7:50230/1‑139 (MQ=255)
          aGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGtttgtttg                                                                                                                    <  7:192959/139‑1 (MQ=255)
               cTGCAGGAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGGCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGc                                                                                                               <  2:283486/139‑1 (MQ=255)
               cTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGATTATGCAATACCCTAGACATTCGGCCCCTTCCGGCTgtttttttttattg                                                                                                               <  1:177909/139‑7 (MQ=255)
                              tcaGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGcc                                                                                                <  8:50631/137‑1 (MQ=255)
                                     cGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCg                                                                                           <  8:50230/137‑1 (MQ=255)
                                     cGGTGTTATTTGGCGCGGAATGACAGGCCGCAAAATCAGGCTCCTCCAACCACCTTTTCCTTCTTGACATTGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCg                                                                                           <  8:437665/137‑1 (MQ=255)
                                      ggTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCGGTTTTTTTGTAAGCCACCACAAGGGTGCCTTCCgggg                                                                                        >  4:89143/1‑137 (MQ=255)
                                       gtgtTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGATAATTCCATACCCTAGACATTCGGCCCCTTCCGGCTGGTTGTTTGTAAGCCACCACAAGGGTGCCTTCCGGTGc                                                                                       >  3:100291/1‑139 (MQ=255)
                                       gtgtTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGc                                                                                       <  3:89143/139‑1 (MQ=255)
                                                              ggCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGttat                                                                                  >  3:66093/1‑121 (MQ=255)
                                                              ggCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGttat                                                                                  <  4:66093/121‑1 (MQ=255)
                                                                 cGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGt                                                                                     >  2:218620/1‑115 (MQ=255)
                                                                 cGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGt                                                                                     <  1:218620/115‑1 (MQ=255)
                                                                 cGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATga                                                                 <  7:103643/135‑2 (MQ=255)
                                                                 cGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATga                                                                 >  8:103643/1‑134 (MQ=255)
                                                                 cGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCg                                                             >  2:292414/1‑139 (MQ=255)
                                                                          aGGCTCATCCAAACTCCGTTTCCTTCTAGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGc                                                     <  3:368997/138‑1 (MQ=255)
                                                                                         ccgtttCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGt                                     <  1:292414/136‑1 (MQ=255)
                                                                                          cgtttCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCTTTTTTTTCGTGCTTTAAAGAACGt                                     >  8:77809/3‑138 (MQ=255)
                                                                                            tttCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGc                                                                                                 <  7:193452/76‑1 (MQ=255)
                                                                                            tttCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGc                                                                                                 >  8:193452/1‑76 (MQ=255)
                                                                                            tttCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTcat                                  >  8:3426/1‑137 (MQ=255)
                                                                                            tttCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACCTcat                                  >  7:285150/1‑137 (MQ=255)
                                                                                                cttctTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATggt                               <  5:9032/136‑3 (MQ=255)
                                                                                                      gagaATGCCATACCCTCGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGgtaaata                          <  2:370288/137‑1 (MQ=255)
                                                                                                      gagaATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGGGTGCTTGAAAGAACGTCATGgtaaat                           >  3:93439/1‑136 (MQ=255)
                                                                                                           tGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGgtaaata                          <  4:196399/132‑1 (MQ=255)
                                                                                                           tGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGgtaaata                          >  3:196399/1‑132 (MQ=255)
                                                                                                                  cccTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGTACGTCATGGTAAATAAGATGCTGGCCCAt            <  1:98030/139‑1 (MQ=255)
                                                                                                                     tAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAATAACGTCATGGTAAATAAGATGCTGGCCCagcac         >  4:378553/1‑135 (MQ=255)
                                                                                                                     tAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCAc         <  6:69217/139‑1 (MQ=255)
                                                                                                                            tCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGtt  >  7:2745/1‑139 (MQ=255)
                                                                                                                            tCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTGGTTTGGGTGCGTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTACGtt  >  4:24353/1‑139 (MQ=255)
                                                                                                                                  |                                                                                                                                    
CCTGCACAACAGCTACTGCAGCAAGTAATGTGAGCTGCGGTGTTTTTTGAAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAATGCCATTTCCCTCTTGAGAATGCCATACCCTAGACATTCGACCTCTGCCGGCTGTTTGTTTGTCAGCCACCACAAGAGTGCCTTCCGGTGCGTTATTATTCGCAAGCTCATGCGCCGTTTTTTGCGTGCTTGAAATAACGTCTTGATAAATAAGATGCTGGCCCATTACTTCCGTT  >  CP006881/2173252‑2173514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑