Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,190,823	T→C	Q182Q (CAA→CAG)	U712_10975 ←	Tryptophan synthase beta chain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,190,823	0	T	C	100.0%	243.2 / NA	74	Q182Q (CAA→CAG)	U712_10975	Tryptophan synthase beta chain
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/33);  total (41/33)

AGGCAGAGTGCCTTCAATGTGTTTCAGCTGATCCTTCGCTTCCTCTCCAATCATTTTCTGAAATTCTCGGACCACCTGCGGATAAGGGTGCGGTCCGACAACTGATCCAATCATGTAAAAATGATCCTCGCAATGTTGAACCCAATACCGAATCGCCTCATTTGTGGCATCCTTCAATGTGCCATTTCCGCTTGTTACAGGAACAACTTCCGCCCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCCTCGCC  >  CP006881/2190688‑2190957                                                                                                                                        |                                                                                                                                       aGGCATAGTGCCTTCAATACGTTTCAACTGATCCTTCGCTTACTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTg                                                                                                                                      <  3:370891/138‑1 (MQ=255)  ggCATAGTGCCTTCAATACGTTTCAACTGATCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGaa                                                                                                                                    <  1:209052/139‑1 (MQ=255)    catagTGCCTTCAATACGTTTCAACTGATCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAAcc                                                                                                                                  <  2:67587/136‑1 (MQ=255)    catagTGCCTTCAATACGTTTCAACTGATCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAAcc                                                                                                                                  <  4:58103/136‑1 (MQ=255)             ttCAATACGTTTCAACTGATCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCg                                                                                                                          >  1:25028/1‑138 (MQ=255)                aatacgtTTCAACTGATCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGAt                                                                                                                       <  3:177137/133‑1 (MQ=255)                  tacgtTTCAACTGATCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGc                                                                                                                    <  8:38618/136‑1 (MQ=255)                  tacgtTTCAACTGATCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGc                                                                                                                    <  2:85962/136‑1 (MQ=255)                       ttCAACTGATCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCAt                                                                                                               >  5:300311/1‑139 (MQ=255)                            cTGATCCTTCGCTTCCTCTACGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTtgtg                                                                                                          <  4:1893/139‑1 (MQ=255)                               aTCCTTCGCTTCCTCTCCGATCGTTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGc                                                                                                        <  8:359594/138‑1 (MQ=255)                               aTCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGCCCACTTGCGGATAAGGATGCGGGCCGCCAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTgg                                                                                                         >  4:269121/1‑137 (MQ=255)                               aTCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGCTCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCa                                                                                                       >  7:375362/1‑139 (MQ=255)                               aTCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCGTTTGTGGCa                                                                                                       <  8:67317/139‑1 (MQ=255)                               aTCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCa                                                                                                       >  4:255577/1‑139 (MQ=255)                               aTCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCa                                                                                                       <  3:255577/139‑1 (MQ=255)                               aTCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCa                                                                                                       <  2:439377/139‑1 (MQ=255)                               aTCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCa                                                                                                       <  2:158267/139‑1 (MQ=255)                               aTCCTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCa                                                                                                       <  1:266115/139‑1 (MQ=255)                                 ccTTCGCTTCCTCTCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATc                                                                                                     >  7:405509/1‑139 (MQ=255)                                           ctctCCGATCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAAtgtt                                                                                           >  3:180842/1‑138 (MQ=255)                                                  aTCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGc                                                                                                                    <  6:146598/107‑1 (MQ=255)                                                  aTCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGc                                                                                                                    >  5:146598/1‑107 (MQ=255)                                                  aTCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTc                                                                                    >  5:394300/1‑139 (MQ=255)                                                  aTCATTTTTTGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTc                                                                                    >  2:417067/1‑139 (MQ=255)                                                      ttttttGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCt                                                                                <  1:417067/139‑1 (MQ=255)                                                        ttttGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGc                                                                                                                    >  8:275826/4‑101 (MQ=255)                                                        ttttGAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGc                                                                                                                    <  7:275826/98‑1 (MQ=255)                                                            gAAATTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTa                                                                           <  1:58541/138‑1 (MQ=255)                                                             aaaTTCACGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACa                                                                         <  4:255024/139‑1 (MQ=255)                                                                   acGGACCACTTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGcac                                                                    <  6:337274/137‑1 (MQ=255)                                                                       accactTGCGGATAAGGATGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGcactacct                                                               >  2:21410/1‑137 (MQ=255)                                                                                      ggatGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACa                                                                         <  3:176492/111‑1 (MQ=255)                                                                                      ggatGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACa                                                                         >  4:176492/4‑114 (MQ=255)                                                                                      ggatGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCt                                                >  2:103245/4‑139 (MQ=255)                                                                                      ggatGCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCt                                                >  1:39586/4‑139 (MQ=255)                                                                                          gCGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCAt                                            >  3:427713/1‑139 (MQ=255)                                                                                           cGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGaa                                                                                                                                    <  8:408300/50‑1 (MQ=255)                                                                                           cGGGCCGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGaa                                                                                                                                    >  7:408300/1‑50 (MQ=255)                                                                                            gggccGACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGc                                          >  7:22630/4‑139 (MQ=255)                                                                                                 gACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGaaa                                     <  6:300311/139‑1 (MQ=255)                                                                                                 gACAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGaa                                      >  2:5265/1‑138 (MQ=255)                                                                                                  aCAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCaag                                                     >  4:237003/1‑122 (MQ=255)                                                                                                  aCAACTGATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCaag                                                     <  3:237003/122‑1 (MQ=255)                                                                                                        gATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAgg                                                                       <  2:222055/98‑1 (MQ=255)                                                                                                        gATCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAgg                                                                       >  1:222055/1‑98 (MQ=255)                                                                                                         aTCCAATCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACATCCTCCGCTCCACGAAGCTTCATGCGGAAAACGtt                                >  2:160209/1‑136 (MQ=255)                                                                                                              aTCATATAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACt                        >  5:256034/1‑139 (MQ=255)                                                                                                                 atatAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGGTACAGGCCCTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGCCTGGc                     >  6:280550/4‑139 (MQ=255)                                                                                                                   atAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCgg                   >  8:385208/2‑139 (MQ=255)                                                                                                                   atAAAAGTGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCgg                   >  1:432761/2‑139 (MQ=255)                                                                                                                        agtGATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAAc              <  2:25028/137‑1 (MQ=255)                                                                                                                          tGATCCTCACAATGCTGAACCCAGGACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACAt            <  6:256034/139‑1 (MQ=255)                                                                                                                           gATCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCCTCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCTGAATACGTTCAGAGACTGGCGGGCAGCAtc           >  7:40735/1‑139 (MQ=255)                                                                                                                            aTCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCg                                                            <  4:394679/89‑1 (MQ=255)                                                                                                                            aTCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCg                                                            <  4:401162/89‑1 (MQ=255)                                                                                                                            aTCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCg                                                            >  3:394679/1‑89 (MQ=255)                                                                                                                            aTCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCg                                                            >  3:401162/1‑89 (MQ=255)                                                                                                                            aTCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAAc              >  5:31170/1‑135 (MQ=255)                                                                                                                            aTCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAAc              <  6:31170/135‑1 (MQ=255)                                                                                                                            aTCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACAtcc          >  8:70659/1‑139 (MQ=255)                                                                                                                            aTCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACAtcc          >  6:8528/1‑139 (MQ=255)                                                                                                                            aTCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACAtcc          >  6:82586/1‑139 (MQ=255)                                                                                                                            aTCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACAtcc          >  6:229740/1‑139 (MQ=255)                                                                                                                            aTCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACAtcc          >  5:220467/1‑139 (MQ=255)                                                                                                                            aTCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACAtcc          >  1:53647/1‑139 (MQ=255)                                                                                                                            aTCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACAgcc          >  5:336105/1‑139 (MQ=255)                                                                                                                            aTCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCAAGAGGAAAACGTTCAGAGACTGGAGGGCAACAtcc          >  6:353773/1‑139 (MQ=255)                                                                                                                            aTCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACATCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACAtcc          >  1:215560/1‑139 (MQ=255)                                                                                                                             tCCTCACAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACAtcct         >  5:447649/1‑139 (MQ=255)                                                                                                                                  acAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACAtcctctt      <  6:220467/136‑3 (MQ=255)                                                                                                                                  acAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCctcttca    <  4:180842/138‑2 (MQ=255)                                                                                                                                   cAATGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCctcttcac   <  3:54979/139‑3 (MQ=255)                                                                                                                                    aaTGCTGAACCCAGTACCGGATCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCAcc  >  1:46844/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                       AGGCAGAGTGCCTTCAATGTGTTTCAGCTGATCCTTCGCTTCCTCTCCAATCATTTTCTGAAATTCTCGGACCACCTGCGGATAAGGGTGCGGTCCGACAACTGATCCAATCATGTAAAAATGATCCTCGCAATGTTGAACCCAATACCGAATCGCCTCATTTGTGGCATCCTTCAATGTGCCATTTCCGCTTGTTACAGGAACAACTTCCGCCCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCCTCGCC  >  CP006881/2190688‑2190957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑