Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,190,970	G→A	C133C (TGC→TGT)	U712_10975 ←	Tryptophan synthase beta chain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,190,970	0	G	A	100.0%	230.3 / NA	73	C133C (TGC→TGT)	U712_10975	Tryptophan synthase beta chain
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/32);  total (41/32)

AATCGCCTCATTTGTGGCATCCTTCAATGTGCCATTTCCGCTTGTTACAGGAACAACTTCCGCCCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCCTCGCCCATAAACACAGTGCAAGAAAAGCCGAATTTGGCTGCGACTGTGGCAGCGGCAACACCGTGCTGACCGGCACCGGTTTCAGCAATGATTTTCGATTTTCCCATTTTCTTAGCGAGCAGCGCTTGACCTAACGCATTATTGATTTTATG  >  CP006881/2190838‑2191104                                                                                                                                     |                                                                                                                                       gaTCGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGa                                                                                                                                   >  8:385077/2‑138 (MQ=255) gaTCGCCTCATTTGTGGCATCCTTCAATGTTACGTTTCCGCTTGTTACAGTAACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGATCAGAGCCTGGCGCGCAACATCCTCTACACCCATAACCACAGTACAGGa                                                                                                                                   >  7:144672/2‑138 (MQ=255)    cGCCTCATTTGTGGCATCCTTCAATGTTCCGTTTCAGCTTGTTACAGGCACTACATCCGCACCAAGAAGCATCATGCGGAAAACGTTCAGAGACTGGATGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAg                                                                                                                               >  1:279255/1‑139 (MQ=255)      ccTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAACCGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGcc                                                                                                                             >  7:210929/1‑139 (MQ=255)      ccTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAg                                                                                                                               <  2:60730/137‑1 (MQ=255)      ccTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAg                                                                                                                               >  1:60730/1‑137 (MQ=255)      ccTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGcc                                                                                                                             >  3:201650/1‑139 (MQ=255)      ccTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGcc                                                                                                                             >  2:83808/1‑139 (MQ=255)      ccTCATTTGTGGCATCCTTCAATGTTCCGTTTCCGCATGTTACAGGCACTACCACCGCTCCAAGAACCTTCATGCCGAAAACGATCAGAGACTGCCCGGCAACATCCACTTCCCCCATACACCCAGTACACGAAAAGcc                                                                                                                             >  2:218050/1‑139 (MQ=255)                  cATCCTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGc                                                                                                                 <  1:21410/139‑1 (MQ=255)                     ccTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAAt                                                                                                                         >  5:422934/1‑128 (MQ=255)                     ccTTCAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAAt                                                                                                                         <  6:422934/128‑1 (MQ=255)                         cAATGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACtgtt                                                                                                          >  8:154102/1‑138 (MQ=255)                           aTGTTCCGTTTCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGc                                                                                                        <  1:160209/139‑1 (MQ=255)                           aTGTTCCGTATCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGc                                                                                                        <  3:257584/139‑1 (MQ=255)                                ccgtttCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCgg                                                                                                   >  5:427085/4‑139 (MQ=255)                                 cgtttCCGCTTGTTACATGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGTCTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCgg                                                                                                   <  5:353773/136‑1 (MQ=255)                                 cgtttCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCgg                                                                                                   <  5:8528/136‑1 (MQ=255)                                 cgtttCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCgg                                                                                                   >  5:300161/3‑138 (MQ=255)                                 cgtttCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCgg                                                                                                   <  1:5265/136‑1 (MQ=255)                                 cgtttCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCgg                                                                                                   <  7:154102/136‑1 (MQ=255)                                 cgtttCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGc                                                                                                  <  2:46844/137‑1 (MQ=255)                                   tttCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGc                                                                                                        <  8:168845/131‑1 (MQ=255)                                   tttCCGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGc                                                                                                        >  7:168845/1‑131 (MQ=255)                                      ccGCTTGTTACAGGCACTACCTCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAaca                                                                                              <  7:385077/138‑1 (MQ=255)                                                       tacctCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCg                                                                            >  7:55137/5‑139 (MQ=255)                                                        acctCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCgg                                                                           >  7:5476/4‑139 (MQ=255)                                                         cctCCGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGt                                                                          <  7:385208/137‑1 (MQ=255)                                                             cGCTCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGttt                                                                        >  3:329490/1‑137 (MQ=255)                                                               ctccAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAg                                                                     >  8:134630/3‑138 (MQ=255)                                                               ctccAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAg                                                                     >  5:211519/3‑138 (MQ=255)                                                               ctccAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTAGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAg                                                                     >  5:141162/3‑138 (MQ=255)                                                                  cAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCa                                                                   <  4:282920/137‑1 (MQ=255)                                                                  cAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAAt                                                                 <  7:419686/139‑1 (MQ=255)                                                                    agaagCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATga                                                               >  5:161084/1‑139 (MQ=255)                                                                         cTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATgattttc                                                          >  6:141260/1‑139 (MQ=255)                                                                            cATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCACCACCATGCTGGCCGGCACCGGTTTCAGCAATgattttcgtt                                                       >  3:279971/1‑137 (MQ=255)                                                                                      aCGTTCAGAGACTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATGATTTTCGTTTTGCCCAttt                                             <  6:394300/139‑1 (MQ=255)                                                                                                tcTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGACAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATGATTTTCGTTTTGCCCATTTTTTTAGCAag                                   <  1:218050/138‑1 (MQ=255)                                                                                                aCTGGCGGGCAACCTCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCAGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATGATTTTCGTTTTGCCCATTTTTTTAGCAag                                   <  6:336105/139‑1 (MQ=255)                                                                                                aCTGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATGATTTTCGTTTTGCCCATTTTTTTAGCAag                                   <  5:432838/139‑1 (MQ=255)                                                                                                  tGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCAc                                                                              <  8:57143/94‑1 (MQ=255)                                                                                                  tGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCAc                                                                              >  7:57143/1‑94 (MQ=255)                                                                                                  tGGCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATGATTTTCGTTTTGCCCATTTTTTTAGCAag                                   >  1:437584/1‑137 (MQ=255)                                                                                                    gCGGGCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATGATTTTCGTTTTGCCCATTTTTTTagcaagca                                 <  7:179449/137‑1 (MQ=255)                                                                                                      gggCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATGATTTTCGTTTTGCCCATTTTTTTAGCAAGCAgcgc                             <  5:280550/139‑1 (MQ=255)                                                                                                      gggCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATGATTTTCGTTTTGCCCATTTTTTTAGCAAGCAgcgc                             <  2:432761/139‑1 (MQ=255)                                                                                                       ggCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATGATTTTCGTTTTGCCCATTTTTTTAGCAAGCAGCGCt                            <  5:229740/139‑1 (MQ=255)                                                                                                       ggCAACATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATGATTTTCGTTTTGCCCATTTTTTTAGCAAGCAGCGCt                            <  3:153750/139‑1 (MQ=255)                                                                                                           aCATCCTCTTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATGATTTTCGTTTTGCCCATTTTTTTAGCAAGCAGCGCTTGAc                        <  1:248966/139‑1 (MQ=255)                                                                                                              tcctctTCACCCATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATGATTTTCGTTTTGCCCATTTTTTTAGCAAGCAGCGCTTGACCTa                     <  5:92864/139‑1 (MQ=255)                                                                                                                   ttcacccATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCCCCGGTTTCAGCAATGATTTTCGTTTTGCCCATTTTTTTAGCAAGCAGCGCTTGACCTAGCGCa                >  2:410118/5‑139 (MQ=255)                                                                                                                   ttcacccATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATGATTTTCGTTTTGCCCATTTTTTTAGCAAGCAGCGCTTGACCTAGCGCa                >  7:148554/5‑139 (MQ=255)                                                                                                                   ttcacccATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATGATTTTCGTTTTGCCCATTTTTTTAGCAAGCAGCGCTTGACCTAGCGCa                >  2:329528/5‑139 (MQ=255)                                                                                                                   ttcacccATAAACACAGTACAGGAAAAGACGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATGATTTTCGTTTTGCCCATTTTTTTAGCAAGCAGCGCTTGACCTAGCg                  <  8:210929/133‑1 (MQ=255)                                                                                                                      acccATAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATGATTTTCGTTTTGCCCATTTTTTTAGCAAGCAGCGCTTGACCTAGCGCatta             >  2:46755/2‑139 (MQ=255)                                                                                                                          aTAAACACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATGATTTTCGTTTTGCCCATTTTTTTAGCAAGCAGCGCTTGACCTAGCGCATTATTGa         >  4:249209/1‑139 (MQ=255)                                                                                                                             aaCACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATGATTTTCGTTTTGCCCATTTTTTTagcaagca                                 <  7:301226/112‑1 (MQ=255)                                                                                                                             aaCACAGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATGATTTTCGTTTTGCCCATTTTTTTagcaagca                                 >  8:301226/1‑112 (MQ=255)                                                                                                                                acaGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATGATTTTCGTTTTGCCCATTTTTTTAGCAAGCAGCGCTTGACCTAGCGCATTATTGATTTTAt   <  2:39586/139‑1 (MQ=255)                                                                                                                                 caGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATGATTTTCGTTTTGCCCATTTTTTTAGCAAGCAGCGCTTGACCTAGCGCATTATTGATTTTATg  <  6:211519/139‑1 (MQ=255)                                                                                                                                 caGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATGATTTTCGTTTTGCCCATTTTTTTAGCAAGCAGCGCTTGACCTAGCGCATTATTGATTTTATg  <  1:301305/139‑1 (MQ=255)                                                                                                                                  aGTACAGGAAAAGCCGAATTTGTCTGCAACTGTTGCAGCGGCAACACCATGCTGGCAGGCACCGGTTTCAGCAATGATTTTCGTTTTGCCCATTTTTTTAGCAAGCATCGCTTGACCTACCGCATAATTGATTTTAt   >  2:164486/1‑137 (MQ=255)                                                                                                                                  aGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATGATTTTCGTTTTGCCCATTTTTTTAGCAAGCAGCGCTTGCCCTACGGCATTATTGATGTTAt   >  2:225474/1‑137 (MQ=255)                                                                                                                                  aGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATGATTTTCGTTTTGCCCATTTTTTTAGCAAGCAGCGCTTGACCTAGCGCATTATTGATTTTAt   >  5:179664/1‑137 (MQ=255)                                                                                                                                  aGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATGATTTTCGTTTTGCCCATTTTTTTAGCAAGCAGCGCTTGACCTAGCGCATTATTGATTTTAt   >  7:366362/1‑137 (MQ=255)                                                                                                                                  aGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATGATTTTCGTTTTGCCCATTTTTTTAGCAAGCAGCGCTTGACCTAGCGCATTATTGATTTTAt   >  4:341722/1‑137 (MQ=255)                                                                                                                                  aGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATGATTTTCGTTTTGCCCATTTTTTTAGCAAGCAGCGCTTGACCTAGCGCATTATTGATTTTAt   >  2:306253/1‑137 (MQ=255)                                                                                                                                  aGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATGATTTTCGTTTTGCCCATTTTTTTAGCAAGCAGCGCTTGACCTAGCGCATTATTGATTTTAt   >  2:131510/1‑137 (MQ=255)                                                                                                                                  aGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATGATTTTCGTTTTGCCCATTTTTTTAGCAAGCAGCGCTTGACCTAGCGCATTATTGATTTTAt   >  3:272270/1‑137 (MQ=255)                                                                                                                                  aGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAATGATTTTCGTTTTCCCCATTTTTTTAGCAAGCAGCGCTAGACCTAGCGCATTAATGAtattat   >  3:208261/1‑137 (MQ=255)                                                                                                                                  aGTACAGGAAAAGCCGAATTTGGCTGCAACTGTTGCAGCGGCAACACCATGCTGGCCGGCACCGGTTTCAGCAAGGATTTTCGTTTTGCCCATTTTTTTAGCAAGCAGCGCTTGACCTAGCGCATTATTGATTTTAt   >  6:68316/1‑137 (MQ=255)                                                                                                                                  aGTACAGGAAAAGCCGAATTTGGCTGCAACTGGTGCAGCGGCAACACCATGCTGGCCGGCACCGGTGTCCGCAATGATGTTCGTTTTGCCCCTTTTTTTAGCAAGCCGCGCTTGACCTAGCGCAGTATTGAGTTTAt   >  5:53235/1‑137 (MQ=255)                                                                                                                                     |                                                                                                                                       AATCGCCTCATTTGTGGCATCCTTCAATGTGCCATTTCCGCTTGTTACAGGAACAACTTCCGCCCCAAGAAGCTTCATGCGGAAAACGTTCAGAGACTGGCGGGCAACATCCTCCTCGCCCATAAACACAGTGCAAGAAAAGCCGAATTTGGCTGCGACTGTGGCAGCGGCAACACCGTGCTGACCGGCACCGGTTTCAGCAATGATTTTCGATTTTCCCATTTTCTTAGCGAGCAGCGCTTGACCTAACGCATTATTGATTTTATG  >  CP006881/2190838‑2191104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑