Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,173,442	A→G	L102L (CTT→CTC)	U712_10870 ←	Bifunctional protein BirA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,173,442	0	A	G	100.0%	113.8 / NA	36	L102L (CTT→CTC)	U712_10870	Bifunctional protein BirA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/17);  total (19/17)

CGCAAAATCAGGCTCATCCAAATGCCATTTCCCTCTTGAGAATGCCATACCCTAGACATTCGACCTCTGCCGGCTGTTTGTTTGTCAGCCACCACAAGAGTGCCTTCCGGTGCGTTATTATTCGCAAGCTCATGCGCCGTTTTTTGCGTGCTTGAAATAACGTCTTGATAAATAAGATGCTGGCCCATTACTTCCGTTTTCAATCCGAAGCGAATTTCACTTTCACTGAGTTTCCCGGGCTTTTTGATAAGCCGATA  >  CP006881/2173317‑2173573                                                                                                                              |                                                                                                                                    cGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATga                                                                                                                            <  7:103643/135‑2 (MQ=255) cGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATga                                                                                                                            >  8:103643/1‑134 (MQ=255) cGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCg                                                                                                                        >  2:292414/1‑139 (MQ=255)          aGGCTCATCCAAACTCCGTTTCCTTCTAGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGc                                                                                                                <  3:368997/138‑1 (MQ=255)                         ccgtttCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGt                                                                                                <  1:292414/136‑1 (MQ=255)                          cgtttCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCTTTTTTTTCGTGCTTTAAAGAACGt                                                                                                >  8:77809/3‑138 (MQ=255)                            tttCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTcat                                                                                             >  8:3426/1‑137 (MQ=255)                            tttCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACCTcat                                                                                             >  7:285150/1‑137 (MQ=255)                                cttctTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATggt                                                                                          <  5:9032/136‑3 (MQ=255)                                      gagaATGCCATACCCTCGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGgtaaata                                                                                     <  2:370288/137‑1 (MQ=255)                                      gagaATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGGGTGCTTGAAAGAACGTCATGgtaaat                                                                                      >  3:93439/1‑136 (MQ=255)                                           tGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGgtaaata                                                                                     >  3:196399/1‑132 (MQ=255)                                           tGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGgtaaata                                                                                     <  4:196399/132‑1 (MQ=255)                                                  cccTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGTACGTCATGGTAAATAAGATGCTGGCCCAt                                                                       <  1:98030/139‑1 (MQ=255)                                                     tAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAATAACGTCATGGTAAATAAGATGCTGGCCCagcac                                                                    >  4:378553/1‑135 (MQ=255)                                                     tAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCAc                                                                    <  6:69217/139‑1 (MQ=255)                                                            tCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGtt                                                             >  7:2745/1‑139 (MQ=255)                                                            tCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTGGTTTGGGTGCGTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTACGtt                                                             >  4:24353/1‑139 (MQ=255)                                                                    ttccGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATc                                                      >  5:182885/3‑138 (MQ=255)                                                                    ttccGGCTGGTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGAGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTTCTTTTAATCACGGGATGGGAAATAATATTCTGGCCCATAACTTCCGTGTTTAAta                                                      >  7:126247/3‑137 (MQ=255)                                                                                         ccaccaCAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATttcgctttcact                                <  6:182885/139‑1 (MQ=255)                                                                                                   ggTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAAtt                                           <  7:94194/117‑1 (MQ=255)                                                                                                   ggTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAAtt                                           >  8:94194/2‑118 (MQ=255)                                                                                                   ggTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTGTTTTGGGTGCTTGAAAGAACGTCATGGTAAATAAGATTCTGGCCCATCACTTCCGTTTTTAATACAAAAAGAATTTCGCTTTCACTGAGttttgcg                      >  3:268365/2‑135 (MQ=255)                                                                                                       ccTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCg                                                                                                               <  4:297768/46‑1 (MQ=255)                                                                                                       ccTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCg                                                                                                               >  3:297768/1‑46 (MQ=255)                                                                                                       ccTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGaaag                                                                                                     <  1:52806/56‑2 (MQ=255)                                                                                                       ccTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGaaag                                                                                                     >  2:52806/1‑55 (MQ=255)                                                                                                       ccTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCgggtt                  >  6:71674/1‑137 (MQ=255)                                                                                                       ccTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTGTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGGTTGCCCATCACTTCCGTTTTTTATGGAAAAAGAATTTTGAGTTCAATGAGTTTTCCggggg                  >  4:43951/1‑137 (MQ=255)                                                                                                       ccTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTGTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGGTGGCCCCTCACTTCCGTTTTTTATCCAAACAGAATTTCGCATTCACTGAGTTTTACgggtt                  >  8:111011/1‑137 (MQ=255)                                                                                                            cGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTg             <  5:284325/139‑1 (MQ=255)                                                                                                              gTGCGTTATTATTCGCGAGCTCATGATCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGATTTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGAt           <  3:26390/139‑1 (MQ=255)                                                                                                               tGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGAtg          <  8:2745/139‑2 (MQ=255)                                                                                                                 cGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAg        <  6:16658/139‑1 (MQ=255)                                                                                                                        attCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGata  <  5:385419/138‑1 (MQ=255)                                                                                                                           cgcgAGCTCATGAGCCGTTTTTTGCGTGCTTGAAATAACGTCATGgtaaata                                                                                     >  6:197633/1‑52 (MQ=255)                                                                                                                           cgcgAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGgtaaata                                                                                     <  5:197633/52‑1 (MQ=255)                                                                                                                              |                                                                                                                                    CGCAAAATCAGGCTCATCCAAATGCCATTTCCCTCTTGAGAATGCCATACCCTAGACATTCGACCTCTGCCGGCTGTTTGTTTGTCAGCCACCACAAGAGTGCCTTCCGGTGCGTTATTATTCGCAAGCTCATGCGCCGTTTTTTGCGTGCTTGAAATAACGTCTTGATAAATAAGATGCTGGCCCATTACTTCCGTTTTCAATCCGAAGCGAATTTCACTTTCACTGAGTTTCCCGGGCTTTTTGATAAGCCGATA  >  CP006881/2173317‑2173573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑