Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,191,215	G→T	H52K (CAT→AAG)	U712_10975 ←	Tryptophan synthase beta chain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,191,215	0	G	T	100.0%	163.5 / NA	52	H52K (CAT→AAG)	U712_10975	Tryptophan synthase beta chain
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (21/31);  total (21/31)

ACCTAACGCATTATTGATTTTATGAGACCCTGTATGGTTTAAATCTTCTCGTTTCAAATAAATTTTCGCGCCGCCTAAGTACTCAGTAACTCGATCAGCAAACGTGAGTGCAGTCGGCCTACCGGAATAGTCATGTAACAGCTTGTAATATTCATTACGAAAAACAGGATCATCCTTGATCTCTTTAAATGCGGTTTCAATTTCTTCTAACGGCTGCATCAGTGTTTCCGGAACAAACTTTCCGCCAAAATCGCCGTATCTGC  >  CP006881/2191081‑2191343                                                                                                                                       |                                                                                                                                 acatagcGCATTATTGATTTTATGTGAAACTGTATGGTTTAAATCTTCTAGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTTATGCAGTCGGGCGTCCGGAATAGTCCTTTaa                                                                                                                               <  6:267925/132‑1 (MQ=255) aCCTAGCGCATTATTGATTTTATGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTaa                                                                                                                               <  5:141260/138‑1 (MQ=255) aCCTAGCGCATTATTGATTTTATGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTaa                                                                                                                               <  1:217717/138‑1 (MQ=255) aCCTAGCGCATTATTGATTTTATGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTaa                                                                                                                               <  6:443852/138‑1 (MQ=255)  ccTAGCGCATTATTGATTTTATGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATTGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACa                                                                                                                             <  8:366362/139‑1 (MQ=255)  ccTAGCGCATTATTGATTTTATGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACa                                                                                                                             <  7:201487/139‑1 (MQ=255)  ccTAGCGCATTATTGATTTTATGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACa                                                                                                                             <  6:169456/139‑1 (MQ=255)  ccTAGCGCATTATTGATTTTATGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACa                                                                                                                             <  1:264008/139‑1 (MQ=255)  ccTAGCGCATTATTGATTATATGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGTCGTCCGGAATAGTCCTTTAACa                                                                                                                             <  8:144672/139‑1 (MQ=255)     agcGCATTATTGATTTTATGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACa                                                                                                                             >  7:12175/3‑136 (MQ=255)     agcGCATTATTGATTTTATGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACa                                                                                                                             <  8:12175/134‑1 (MQ=255)     agcGCATTATTGATTTTATGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTAAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCt                                                                                                                          >  1:361781/3‑139 (MQ=255)      gcGCATTATTGATTTTATGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGc                                                                                                                           <  7:268703/136‑1 (MQ=255)      gcGCATTATTGATTTTATGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGc                                                                                                                           <  3:28107/136‑1 (MQ=255)      gcGCATTATTGATTTTATGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGc                                                                                                                           <  5:68316/136‑1 (MQ=255)         cATTATTGATTTTATGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTtata                                                                                                                      <  5:82586/139‑1 (MQ=255)         cATTATTGATTTTATGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTtata                                                                                                                      <  8:55137/139‑1 (MQ=255)            tattGATTTTATGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAg                                                                                                                            >  8:61639/1‑130 (MQ=255)            tattGATTTTATGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAg                                                                                                                            <  7:61639/130‑1 (MQ=255)            tattGATTTTATGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTataata                                                                                                                   >  4:387364/1‑139 (MQ=255)            tattGATTTTATGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTataata                                                                                                                   >  7:29575/1‑139 (MQ=255)             attGATTTTATGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACa                                                                                                                             <  7:307694/128‑1 (MQ=255)             attGATTTTATGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACa                                                                                                                             >  8:307694/1‑128 (MQ=255)                   tttATGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTcttca                                                                                                            >  4:66923/1‑135 (MQ=255)                      aTGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACg                                                                                                          >  8:368066/1‑138 (MQ=255)                       tGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGa                                                                                                         <  1:306253/138‑1 (MQ=255)                           aaccTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTaa                                                                                                                               >  7:276129/3‑112 (MQ=255)                           aaccTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTaa                                                                                                                               <  8:276129/110‑1 (MQ=255)                                gTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACa                                                                                                                             <  1:378532/109‑1 (MQ=255)                                gTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACa                                                                                                                             >  2:378532/1‑109 (MQ=255)                                 tATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGat                                                                                               <  7:368066/138‑1 (MQ=255)                                 tATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGat                                                                                               <  5:295847/138‑1 (MQ=255)                                     gTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACg                                                                                                          <  5:446317/123‑1 (MQ=255)                                     gTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACg                                                                                                          >  6:446317/1‑123 (MQ=255)                                      tttAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATACTACTCGTCACGAATAGCGGGATCATCCt                                                                                         >  4:398938/1‑139 (MQ=255)                                        tAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCtt                                                                                        >  3:187940/1‑138 (MQ=255)                                        tAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCtt                                                                                        >  3:287264/1‑138 (MQ=255)                                           ttcttctCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTGAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTGTCGAAAATCTGGATCATCCTTGAtt                                                                                    <  4:208261/138‑2 (MQ=255)                                           aTCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGAtt                                                                                    <  4:287264/139‑2 (MQ=255)                                                ctcGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGAtttgtt                                                                                >  7:35339/1‑133 (MQ=255)                                                ctcGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGAtttgtt                                                                                >  3:4694/1‑133 (MQ=255)                                                ctcGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGAtttg                                                                                  <  6:179664/136‑4 (MQ=255)                                                        aaatagatTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGATTTGTTTAAATGctg                                                                       >  6:292711/1‑137 (MQ=255)                                                        aaatagatTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGATTTGTTTAAATGct                                                                        <  3:66923/138‑2 (MQ=255)                                                                tttCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCGTTGATTTGTTTAAATGCTGtt                                                                     >  1:36860/1‑133 (MQ=255)                                                                                                        gttaatGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGATTTGTTTAAATGCTGTTTGTATTTCATCTAACGGCTGCATGAGTGTTTCCGGAACAAACTTTc                       >  3:448431/6‑139 (MQ=255)                                                                                                             tGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGATTTGTTTAAATGCTGTTTGTATTTCATCTAAGGGGTGCTTGAGTGTTACCGGAACAAACTTTCCGcc                   >  2:45147/1‑138 (MQ=255)                                                                                                             tGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGATTTGTTTAAATGCTGTTTGTATTTCATATAACGGCTGCATGAGTGTTTCCGGAACAAACTTTCCGcc                   >  8:232538/1‑138 (MQ=255)                                                                                                                         tccGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGATTTGTTTAAATGCTGTTTGTATTTCATCTAACGGCTGCATGAGTGTTTCCGGAACAAACTTTCCGCCAAAATCACCGTAt      <  3:53854/138‑1 (MQ=255)                                                                                                                         tccGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGATTTGTTTAAATGCTGTTTGTATTTCATCTAACGGCTGCATGAGTGTTTCCGGAACAAACTTTCCGCCAAAATCACCGTAt      <  7:314587/138‑1 (MQ=255)                                                                                                                         tccGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGATTTGTTTAAATGCTGTTTGTATTTCATCTAACGGCTGCATGAGTGTTTCCGGAACAAACTTTCCGCCAAAATCACCGTAt      <  5:292711/138‑1 (MQ=255)                                                                                                                             gAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGATTTGTTTAAATGCTGTTTGTATTTCATCTAACGGCTGCATGAGTGTTTCCGGAACAAACTTTCCGCCAAAATCACCGTATCTGc  <  4:446522/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                 ACCTAACGCATTATTGATTTTATGAGACCCTGTATGGTTTAAATCTTCTCGTTTCAAATAAATTTTCGCGCCGCCTAAGTACTCAGTAACTCGATCAGCAAACGTGAGTGCAGTCGGCCTACCGGAATAGTCATGTAACAGCTTGTAATATTCATTACGAAAAACAGGATCATCCTTGATCTCTTTAAATGCGGTTTCAATTTCTTCTAACGGCTGCATCAGTGTTTCCGGAACAAACTTTCCGCCAAAATCGCCGTATCTGC  >  CP006881/2191081‑2191343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑