Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,191,225 G→A Y48Y (TAC→TAT)  U712_10975 ← Tryptophan synthase beta chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,191,2250GA100.0% 125.9 / NA 41Y48Y (TAC→TAT) U712_10975Tryptophan synthase beta chain
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/19);  total (22/19)

CATTATTGATTTTATGAGACCCTGTATGGTTTAAATCTTCTCGTTTCAAATAAATTTTCGCGCCGCCTAAGTACTCAGTAACTCGATCAGCAAACGTGAGTGCAGTCGGCCTACCGGAATAGTCATGTAACAGCTTGTAATATTCATTACGAAAAACAGGATCATCCTTGATCTCTTTAAATGCGGTTTCAATTTCTTCTAACGGCTGCATCAGTGTTTCCGGAACAAACTTTCCGCCAAAATCGCCGTATCTGCCTATTTCATTCGGATAT  >  CP006881/2191089‑2191360
                                                                                                                                        |                                                                                                                                       
cATTATTGATTTTATGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTtata                                                                                                                                       <  8:55137/139‑1 (MQ=255)
cATTATTGATTTTATGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTtata                                                                                                                                       <  5:82586/139‑1 (MQ=255)
   tattGATTTTATGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTataata                                                                                                                                    >  7:29575/1‑139 (MQ=255)
   tattGATTTTATGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTataata                                                                                                                                    >  4:387364/1‑139 (MQ=255)
          tttATGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTcttca                                                                                                                             >  4:66923/1‑135 (MQ=255)
             aTGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACg                                                                                                                           >  8:368066/1‑138 (MQ=255)
              tGAGAACCTGTATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGa                                                                                                                          <  1:306253/138‑1 (MQ=255)
                        tATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGat                                                                                                                <  7:368066/138‑1 (MQ=255)
                        tATGGTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGat                                                                                                                <  5:295847/138‑1 (MQ=255)
                            gTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACg                                                                                                                           >  6:446317/1‑123 (MQ=255)
                            gTTTAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACg                                                                                                                           <  5:446317/123‑1 (MQ=255)
                             tttAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATACTACTCGTCACGAATAGCGGGATCATCCt                                                                                                          >  4:398938/1‑139 (MQ=255)
                               tAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCtt                                                                                                         >  3:187940/1‑138 (MQ=255)
                               tAAATCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCtt                                                                                                         >  3:287264/1‑138 (MQ=255)
                                  ttcttctCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTGAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTGTCGAAAATCTGGATCATCCTTGAtt                                                                                                     <  4:208261/138‑2 (MQ=255)
                                  aTCTTCTCGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGAtt                                                                                                     <  4:287264/139‑2 (MQ=255)
                                       ctcGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGAtttgtt                                                                                                 >  7:35339/1‑133 (MQ=255)
                                       ctcGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGAtttgtt                                                                                                 >  3:4694/1‑133 (MQ=255)
                                       ctcGTTTCAAATAGATTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGAtttg                                                                                                   <  6:179664/136‑4 (MQ=255)
                                               aaatagatTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGATTTGTTTAAATGctg                                                                                        >  6:292711/1‑137 (MQ=255)
                                               aaatagatTTTCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGATTTGTTTAAATGct                                                                                         <  3:66923/138‑2 (MQ=255)
                                                       tttCGCGCCGCCTAAGTATTCAGTGACTCGATCAGCGTATGTTAATGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCGTTGATTTGTTTAAATGCTGtt                                                                                      >  1:36860/1‑133 (MQ=255)
                                                                                               gttaatGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGATTTGTTTAAATGCTGTTTGTATTTCATCTAACGGCTGCATGAGTGTTTCCGGAACAAACTTTc                                        >  3:448431/6‑139 (MQ=255)
                                                                                                    tGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGATTTGTTTAAATGCTGTTTGTATTTCATCTAAGGGGTGCTTGAGTGTTACCGGAACAAACTTTCCGcc                                    >  2:45147/1‑138 (MQ=255)
                                                                                                    tGCAGTCGGGCGTCCGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGATTTGTTTAAATGCTGTTTGTATTTCATATAACGGCTGCATGAGTGTTTCCGGAACAAACTTTCCGcc                                    >  8:232538/1‑138 (MQ=255)
                                                                                                                tccGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGATTTGTTTAAATGCTGTTTGTATTTCATCTAACGGCTGCATGAGTGTTTCCGGAACAAACTTTCCGCCAAAATCACCGTAt                       <  3:53854/138‑1 (MQ=255)
                                                                                                                tccGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGATTTGTTTAAATGCTGTTTGTATTTCATCTAACGGCTGCATGAGTGTTTCCGGAACAAACTTTCCGCCAAAATCACCGTAt                       <  7:314587/138‑1 (MQ=255)
                                                                                                                tccGGAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGATTTGTTTAAATGCTGTTTGTATTTCATCTAACGGCTGCATGAGTGTTTCCGGAACAAACTTTCCGCCAAAATCACCGTAt                       <  5:292711/138‑1 (MQ=255)
                                                                                                                    gAATAGTCCTTTAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGATTTGTTTAAATGCTGTTTGTATTTCATCTAACGGCTGCATGAGTGTTTCCGGAACAAACTTTCCGCCAAAATCACCGTATCTGc                   <  4:446522/139‑1 (MQ=255)
                                                                                                                         gtcctttAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGATTTGTTTAAATGCTGTTTGTATTTCATCTAACGGCTGCATGAGTGTTTCCGGAACAAACTTTCCGCCAAAATCACCGTATCTGCCTAt               <  2:213944/132‑1 (MQ=255)
                                                                                                                          tcctttAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGATTTGTTTAAATGCTGTTTGTATTTCATCTAACGGCTGCATGAGTGTTTCCGGAACAAACTTTCCGc                                     >  4:396569/6‑115 (MQ=255)
                                                                                                                          tcctttAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGATTTGTTTAAATGCTGTTTGTATTTCATCTAACGGCTGCATGAGTGTTTCCGGAACAAACTTTCCGc                                     <  3:396569/110‑1 (MQ=255)
                                                                                                                          tcctttAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGATTTGTTTAAATGCTGTTTGTATTTCATCTAACGGCTGCATGAGTGTTTCCGGAACAAACTTTCCGCCAAAATCACCGTATCTGCCTAttt             >  4:321371/6‑139 (MQ=255)
                                                                                                                            ctttAACAGCTTATAATACTCTTCACGAAAAGCGGCATAATCCATGATAAGTATAAAATCTGTTTGTATTTCATCTAACGGCTGCATGAGTGTTTCCGGAACAAACTTTCCGCAAAAATCACCGTATCATCATATTTCa           >  2:61074/4‑139 (MQ=255)
                                                                                                                               tAACAGCTTATAATACTCTTCACGTAAAGCGGGATCATCCTTGATTTGTTTAAATGCTGTTTGTAATTGATCAAACGGCTGCATGAGTGTTTCCGGAACAAACTTTCCGCCAAAATCACCGTATCTGCCTATTTCAt          <  1:45147/137‑1 (MQ=255)
                                                                                                                               tAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGATTTGTTTAAATGCTGTTTGTATTTCATCTAACGGCTGCATGAGTGTTTCCGGAACAAACTTTCCGCCAAAATCACCGTATCTGCCTATTTCAtt         <  4:4694/138‑1 (MQ=255)
                                                                                                                               tAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGATTTGTTTAAATGCTGTTTGTATTTCATCTAACGGCTGCATGAGTGTTTCCGGAACAAACTTTCCGCCAAAATCACCGTATCTGCCTATTTCATTc        >  5:265873/1‑139 (MQ=255)
                                                                                                                               tAACAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGATTTGTTTAAATGCTGTTTGTATTTCATCTAACGGCTGCATGAGTGTTTCCGGAACAAACTTTCCGCCAAAATCACCGTATCTGCCTATTTCATTc        >  2:433704/1‑139 (MQ=255)
                                                                                                                                 aCAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGATTTGTTTAAATGCTGTTTGTATTTCATCTAACGGCTGCATGAGTGTTTCCGGAACAAACTTTCCGCCAAAATCACCGTATCTGCCTATTTCATTCg       <  6:265873/138‑1 (MQ=255)
                                                                                                                                 aCAGCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGATTTGTTTAAATGCTGTTTGTATTTCATCTAACGGCTGCATGAGTGTTTCCGGAACAAACTTTCCGCCAAAATCACCGTATCTGCCTATTTCATTCg       <  3:341722/138‑1 (MQ=255)
                                                                                                                                    gCTTATAATACTCTTCACGAAGAGCGGGTTCATCCTTGATTTGTTTATATGCTGTTTGTATTTCATCTCTGGGCTGCATGAGTGTTTCCGGAACAAACTTTCCGCCATAATCTCGGTATCTGCCTATTTCATTCGtata   >  1:278806/1‑139 (MQ=255)
                                                                                                                                    gCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGATTTGTTTAAATGCTGTTTGTATTTCATCTAACGGCTGCATGAGTGTTTCCGGAACAAACTTTCCGCCAAAATCACCGTATCTGCCTATTTCATTCGGata   >  6:397620/1‑139 (MQ=255)
                                                                                                                                    gCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGATTTGTTTAAATGCTGTTTGTATTTCATCTAACGGCTGCATGAGTGTTTCCGGAACAAACTTTCCGCCAAAATCACCGTATCTGCCTATTTCATTCGGata   <  5:207701/139‑1 (MQ=255)
                                                                                                                                    gCTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGATTTGTTTAAATGCTGTTTGTATTTCATCTAACGGCTGCATGAGTGTTTCCGGAACAAACTTTCCGCCAAAATCACCGTATCTGCCTATTTCATTCGGata   >  4:297154/1‑139 (MQ=255)
                                                                                                                                     cTTATAATACTCTTCACGAAAAGCGGGATCATCCTTGATTTGTTTAAATGCTGTTTGTATTTCATCTAACGGCTGCATGAGTGTTTCCTGAACAAACTTTCAGCCAAAATCACCGTATCTGCCTATTTCATTCTGatat  >  6:61266/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                       
CATTATTGATTTTATGAGACCCTGTATGGTTTAAATCTTCTCGTTTCAAATAAATTTTCGCGCCGCCTAAGTACTCAGTAACTCGATCAGCAAACGTGAGTGCAGTCGGCCTACCGGAATAGTCATGTAACAGCTTGTAATATTCATTACGAAAAACAGGATCATCCTTGATCTCTTTAAATGCGGTTTCAATTTCTTCTAACGGCTGCATCAGTGTTTCCGGAACAAACTTTCCGCCAAAATCGCCGTATCTGCCTATTTCATTCGGATAT  >  CP006881/2191089‑2191360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑