Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,173,451 C→A A99A (GCG→GCT)  U712_10870 ← Bifunctional protein BirA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,173,4510CA100.0% 120.9 / NA 41A99A (GCG→GCT) U712_10870Bifunctional protein BirA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (20/21);  total (20/21)

CGCAAAATCAGGCTCATCCAAATGCCATTTCCCTCTTGAGAATGCCATACCCTAGACATTCGACCTCTGCCGGCTGTTTGTTTGTCAGCCACCACAAGAGTGCCTTCCGGTGCGTTATTATTCGCAAGCTCATGCGCCGTTTTTTGCGTGCTTGAAATAACGTCTTGATAAATAAGATGCTGGCCCATTACTTCCGTTTTCAATCCGAAGCGAATTTCACTTTCACTGAGTTTCCCGGGCTTTTTGATAAGCCGATATCCTTTTCTTCTA  >  CP006881/2173317‑2173586
                                                                                                                                      |                                                                                                                                       
cGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCg                                                                                                                                     >  2:292414/1‑139 (MQ=255)
         aGGCTCATCCAAACTCCGTTTCCTTCTAGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGc                                                                                                                             <  3:368997/138‑1 (MQ=255)
                        ccgtttCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGt                                                                                                             <  1:292414/136‑1 (MQ=255)
                         cgtttCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCTTTTTTTTCGTGCTTTAAAGAACGt                                                                                                             >  8:77809/3‑138 (MQ=255)
                           tttCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTcat                                                                                                          >  8:3426/1‑137 (MQ=255)
                           tttCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACCTcat                                                                                                          >  7:285150/1‑137 (MQ=255)
                               cttctTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATggt                                                                                                       <  5:9032/136‑3 (MQ=255)
                                     gagaATGCCATACCCTCGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGgtaaata                                                                                                  <  2:370288/137‑1 (MQ=255)
                                     gagaATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGGGTGCTTGAAAGAACGTCATGgtaaat                                                                                                   >  3:93439/1‑136 (MQ=255)
                                          tGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGgtaaata                                                                                                  <  4:196399/132‑1 (MQ=255)
                                          tGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGgtaaata                                                                                                  >  3:196399/1‑132 (MQ=255)
                                                 cccTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGTACGTCATGGTAAATAAGATGCTGGCCCAt                                                                                    <  1:98030/139‑1 (MQ=255)
                                                    tAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAATAACGTCATGGTAAATAAGATGCTGGCCCagcac                                                                                 >  4:378553/1‑135 (MQ=255)
                                                    tAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCAc                                                                                 <  6:69217/139‑1 (MQ=255)
                                                           tCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGtt                                                                          >  7:2745/1‑139 (MQ=255)
                                                           tCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTGGTTTGGGTGCGTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTACGtt                                                                          >  4:24353/1‑139 (MQ=255)
                                                                   ttccGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATc                                                                   >  5:182885/3‑138 (MQ=255)
                                                                   ttccGGCTGGTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGAGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTTCTTTTAATCACGGGATGGGAAATAATATTCTGGCCCATAACTTCCGTGTTTAAta                                                                   >  7:126247/3‑137 (MQ=255)
                                                                                        ccaccaCAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATttcgctttcact                                             <  6:182885/139‑1 (MQ=255)
                                                                                                  ggTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAAtt                                                        >  8:94194/2‑118 (MQ=255)
                                                                                                  ggTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAAtt                                                        <  7:94194/117‑1 (MQ=255)
                                                                                                  ggTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTGTTTTGGGTGCTTGAAAGAACGTCATGGTAAATAAGATTCTGGCCCATCACTTCCGTTTTTAATACAAAAAGAATTTCGCTTTCACTGAGttttgcg                                   >  3:268365/2‑135 (MQ=255)
                                                                                                      ccTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCg                                                                                                                            >  3:297768/1‑46 (MQ=255)
                                                                                                      ccTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCg                                                                                                                            <  4:297768/46‑1 (MQ=255)
                                                                                                      ccTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGaaag                                                                                                                  <  1:52806/56‑2 (MQ=255)
                                                                                                      ccTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGaaag                                                                                                                  >  2:52806/1‑55 (MQ=255)
                                                                                                      ccTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCgggtt                               >  6:71674/1‑137 (MQ=255)
                                                                                                      ccTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTGTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGGTTGCCCATCACTTCCGTTTTTTATGGAAAAAGAATTTTGAGTTCAATGAGTTTTCCggggg                               >  4:43951/1‑137 (MQ=255)
                                                                                                      ccTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTGTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGGTGGCCCCTCACTTCCGTTTTTTATCCAAACAGAATTTCGCATTCACTGAGTTTTACgggtt                               >  8:111011/1‑137 (MQ=255)
                                                                                                           cGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTg                          <  5:284325/139‑1 (MQ=255)
                                                                                                             gTGCGTTATTATTCGCGAGCTCATGATCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGATTTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGAt                        <  3:26390/139‑1 (MQ=255)
                                                                                                              tGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGAtg                       <  8:2745/139‑2 (MQ=255)
                                                                                                                cGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAg                     <  6:16658/139‑1 (MQ=255)
                                                                                                                       attCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGata               <  5:385419/138‑1 (MQ=255)
                                                                                                                          cgcgAGCTCATGAGCCGTTTTTTGCGTGCTTGAAATAACGTCATGgtaaata                                                                                                  >  6:197633/1‑52 (MQ=255)
                                                                                                                          cgcgAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGgtaaata                                                                                                  <  5:197633/52‑1 (MQ=255)
                                                                                                                                  cATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATGAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTttcttc    >  3:128616/1‑138 (MQ=255)
                                                                                                                                   aTGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTa  <  3:146728/139‑1 (MQ=255)
                                                                                                                                   aTGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTa  <  1:158952/139‑1 (MQ=255)
                                                                                                                                   aTGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTa  <  5:71674/139‑1 (MQ=255)
                                                                                                                                   aTGAGCCGTTTTTTGCGCGCTTGAAAGAACGTCATGGTAAATAAGATGCGGGCCCCTCACTTCCGTCTTTAATCCAAAACGAATTTCGCTTTCACTGAGGTTTCCGGGTGTTTTGATGAGCCGATATCCTTTTCTTCTa  <  8:44210/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                       
CGCAAAATCAGGCTCATCCAAATGCCATTTCCCTCTTGAGAATGCCATACCCTAGACATTCGACCTCTGCCGGCTGTTTGTTTGTCAGCCACCACAAGAGTGCCTTCCGGTGCGTTATTATTCGCAAGCTCATGCGCCGTTTTTTGCGTGCTTGAAATAACGTCTTGATAAATAAGATGCTGGCCCATTACTTCCGTTTTCAATCCGAAGCGAATTTCACTTTCACTGAGTTTCCCGGGCTTTTTGATAAGCCGATATCCTTTTCTTCTA  >  CP006881/2173317‑2173586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑