Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,173,474 T→G I92L (ATT→CTT)  U712_10870 ← Bifunctional protein BirA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,173,4740TG93.3% 131.7 / ‑5.6 45I92L (ATT→CTT) U712_10870Bifunctional protein BirA
Reads supporting (aligned to +/- strand):  ref base T (3/0);  new base G (18/24);  total (21/24)
Fisher's exact test for biased strand distribution p-value = 9.37e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCATTTCCCTCTTGAGAATGCCATACCCTAGACATTCGACCTCTGCCGGCTGTTTGTTTGTCAGCCACCACAAGAGTGCCTTCCGGTGCGTTATTATTCGCAAGCTCATGCGCCGTTTTTTGCGTGCTTGAAATAACGTCTTGATAAATAAGATGCTGGCCCATTACTTCCGTTTTCAATCCGAAGCGAATTTCACTTTCACTGAGTTTCCCGGGCTTTTTGATAAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAGCCCT  >  CP006881/2173341‑2173608
                                                                                                                                     |                                                                                                                                      
ccgtttCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGt                                                                                                                                   <  1:292414/136‑1 (MQ=255)
 cgtttCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCTTTTTTTTCGTGCTTTAAAGAACGt                                                                                                                                   >  8:77809/3‑138 (MQ=255)
   tttCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTcat                                                                                                                                >  8:3426/1‑137 (MQ=255)
   tttCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACCTcat                                                                                                                                >  7:285150/1‑137 (MQ=255)
       cttctTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATggt                                                                                                                             <  5:9032/136‑3 (MQ=255)
             gagaATGCCATACCCTCGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGgtaaata                                                                                                                        <  2:370288/137‑1 (MQ=255)
             gagaATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGGGTGCTTGAAAGAACGTCATGgtaaat                                                                                                                         >  3:93439/1‑136 (MQ=255)
                  tGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGgtaaata                                                                                                                        >  3:196399/1‑132 (MQ=255)
                  tGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGgtaaata                                                                                                                        <  4:196399/132‑1 (MQ=255)
                         cccTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGTACGTCATGGTAAATAAGATGCTGGCCCAt                                                                                                          <  1:98030/139‑1 (MQ=255)
                            tAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAATAACGTCATGGTAAATAAGATGCTGGCCCagcac                                                                                                       >  4:378553/1‑135 (MQ=255)
                            tAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCAc                                                                                                       <  6:69217/139‑1 (MQ=255)
                                   tCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGtt                                                                                                >  7:2745/1‑139 (MQ=255)
                                   tCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTGGTTTGGGTGCGTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTACGtt                                                                                                >  4:24353/1‑139 (MQ=255)
                                           ttccGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATc                                                                                         >  5:182885/3‑138 (MQ=255)
                                           ttccGGCTGGTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGAGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTTCTTTTAATCACGGGATGGGAAATAATATTCTGGCCCATAACTTCCGTGTTTAAta                                                                                         >  7:126247/3‑137 (MQ=255)
                                                                ccaccaCAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATttcgctttcact                                                                   <  6:182885/139‑1 (MQ=255)
                                                                          ggTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAAtt                                                                              >  8:94194/2‑118 (MQ=255)
                                                                          ggTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAAtt                                                                              <  7:94194/117‑1 (MQ=255)
                                                                          ggTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTGTTTTGGGTGCTTGAAAGAACGTCATGGTAAATAAGATTCTGGCCCATCACTTCCGTTTTTAATACAAAAAGAATTTCGCTTTCACTGAGttttgcg                                                         >  3:268365/2‑135 (MQ=255)
                                                                              ccTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCgggtt                                                     >  6:71674/1‑137 (MQ=255)
                                                                              ccTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTGTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGGTTGCCCATCACTTCCGTTTTTTATGGAAAAAGAATTTTGAGTTCAATGAGTTTTCCggggg                                                     >  4:43951/1‑137 (MQ=255)
                                                                              ccTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTGTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGGTGGCCCCTCACTTCCGTTTTTTATCCAAACAGAATTTCGCATTCACTGAGTTTTACgggtt                                                     >  8:111011/1‑137 (MQ=255)
                                                                                   cGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTg                                                <  5:284325/139‑1 (MQ=255)
                                                                                     gTGCGTTATTATTCGCGAGCTCATGATCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGATTTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGAt                                              <  3:26390/139‑1 (MQ=255)
                                                                                      tGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGAtg                                             <  8:2745/139‑2 (MQ=255)
                                                                                        cGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAg                                           <  6:16658/139‑1 (MQ=255)
                                                                                               attCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGata                                     <  5:385419/138‑1 (MQ=255)
                                                                                                  cgcgAGCTCATGAGCCGTTTTTTGCGTGCTTGAAATAACGTCATGgtaaata                                                                                                                        >  6:197633/1‑52 (MQ=255)
                                                                                                  cgcgAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGgtaaata                                                                                                                        <  5:197633/52‑1 (MQ=255)
                                                                                                          cATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATGAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTttcttc                          >  3:128616/1‑138 (MQ=255)
                                                                                                           aTGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTa                        <  5:71674/139‑1 (MQ=255)
                                                                                                           aTGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTa                        <  1:158952/139‑1 (MQ=255)
                                                                                                           aTGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTa                        <  3:146728/139‑1 (MQ=255)
                                                                                                           aTGAGCCGTTTTTTGCGCGCTTGAAAGAACGTCATGGTAAATAAGATGCGGGCCCCTCACTTCCGTCTTTAATCCAAAACGAATTTCGCTTTCACTGAGGTTTCCGGGTGTTTTGATGAGCCGATATCCTTTTCTTCTa                        <  8:44210/139‑1 (MQ=255)
                                                                                                                   ttttttGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTGGGTTTCAATGAGTTTTACGGGTTTTTTTATGAGCCGATATCCTTTTATTATAACGGCTTc                >  6:143087/1‑139 (MQ=255)
                                                                                                                   ttttttGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTc                <  4:93439/139‑1 (MQ=255)
                                                                                                                      tttGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTa              <  1:246915/138‑1 (MQ=255)
                                                                                                                          cGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCa         >  7:68288/1‑139 (MQ=255)
                                                                                                                             gCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTa                        <  2:85996/121‑1 (MQ=255)
                                                                                                                             gCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTa                        >  1:85996/1‑121 (MQ=255)
                                                                                                                              cTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTc                <  6:75035/128‑1 (MQ=255)
                                                                                                                              cTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTc                >  5:75035/1‑128 (MQ=255)
                                                                                                                                  aaaGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCct  <  7:77809/138‑1 (MQ=255)
                                                                                                                                  aaaGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCct  <  2:106015/138‑1 (MQ=255)
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CCATTTCCCTCTTGAGAATGCCATACCCTAGACATTCGACCTCTGCCGGCTGTTTGTTTGTCAGCCACCACAAGAGTGCCTTCCGGTGCGTTATTATTCGCAAGCTCATGCGCCGTTTTTTGCGTGCTTGAAATAACGTCTTGATAAATAAGATGCTGGCCCATTACTTCCGTTTTCAATCCGAAGCGAATTTCACTTTCACTGAGTTTCCCGGGCTTTTTGATAAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAGCCCT  >  CP006881/2173341‑2173608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑