Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,191,474	A→G	L175L (TTA→CTA)	U712_10980 ←	N‑(5'‑phosphoribosyl)anthranilate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,191,474	0	A	G	100.0%	126.4 / NA	40	L175L (TTA→CTA)	U712_10980	N‑(5'‑phosphoribosyl)anthranilate isomerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/18);  total (22/18)

TTTCATTCGGATATGGATACATATCGATTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCGATTCCGCTGGCAAGGTCAATTCCTGCCGGCTGATATTTCATTAAGTCTGTGATAGTGTCCGGATTCACGCCGCCTGCGATAAAACAACGTTTTCCTATAGCAGCCTGCTGGTATTCCGGCACACAGTCCCAAGAAAAAGCAACACCAGTGCCCCCTCTTGCCCCTTTGACA  >  CP006881/2191347‑2191601                                                                                                                                |                                                                                                                                tttCATTCGGATATGGATACATATCGGTTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGAtgc                                                                                                                      >  1:64799/1‑137 (MQ=255) tttCATTCGGATATGGATACATATCGGTTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGAtg                                                                                                                       <  1:136411/138‑2 (MQ=255)           atatGGATACATATCGGTTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGAGCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCGATTTCAATAGGCGTGTGATGCTATCCGGAt                                                                                                             >  6:179982/1‑138 (MQ=255)                    cATATCGGTTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACgccgcc                                                                                                   <  7:250890/139‑1 (MQ=255)                    cATATCGGTTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACgccgcc                                                                                                   <  5:2378/139‑1 (MQ=255)                     atatCGGTTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCAcgccgc                                                                                                    >  4:241861/1‑137 (MQ=255)                     atatCGGTTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCAcgccgc                                                                                                    >  7:35158/1‑137 (MQ=255)                     atatCGGTTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCAcgccgc                                                                                                    >  3:53203/1‑137 (MQ=255)                     atatCGGTTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCAcgccgc                                                                                                    >  3:21322/1‑137 (MQ=255)                      tatCGGTTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACgccgcc                                                                                                   <  4:53203/137‑1 (MQ=255)                       atCGGTTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGc                                                                                                >  8:48344/1‑139 (MQ=255)                       atCGGTTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGc                                                                                                >  7:20314/1‑139 (MQ=255)                          ggttCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGa                                                                                              <  1:45447/136‑1 (MQ=255)                          ggttCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGGGATGCTATCCGGATTCACGCCGCCAGCGa                                                                                              <  4:275538/136‑1 (MQ=255)                           gttCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGAtg                                                                                                                       >  7:161606/2‑111 (MQ=255)                           gttCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGAtg                                                                                                                       <  8:161606/111‑2 (MQ=255)                           gttCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGAt                                                                                             >  6:87644/2‑138 (MQ=255)                               aTCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATaaagc                                                                                        >  1:101943/1‑137 (MQ=255)                               aTCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATaaag                                                                                         >  6:409972/1‑137 (MQ=255)                               aTCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATaaag                                                                                         <  1:103196/138‑2 (MQ=255)                                 ccTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAagcag                                                                                      >  1:14608/1‑138 (MQ=255)                                     tcttctAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCg                                                                                    <  8:20314/137‑1 (MQ=255)                                          tAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTAcc                                                                              >  2:372856/1‑138 (MQ=255)                                                 cTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCAcg                                                                                                        >  2:271391/1‑105 (MQ=255)                                                 cTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCAcg                                                                                                        <  1:271391/105‑1 (MQ=255)                                                   catcaGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAgccgcc                                                                    >  1:163267/1‑139 (MQ=255)                                                    atcaGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAgccgcc                                                                    <  1:372856/138‑1 (MQ=255)                                                    atcaGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGACGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAgccgcc                                                                    <  8:84025/138‑1 (MQ=255)                                                        gATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCctgctg                                                               <  8:375163/139‑1 (MQ=255)                                                           tCTGAGCTTTTTGTCCGGTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGCTGCTTTCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATa                                                            >  3:45375/1‑139 (MQ=255)                                                              gATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTc                                                         >  2:9665/1‑139 (MQ=255)                                                                    tttGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGca                                                    >  7:97280/1‑138 (MQ=255)                                                                          cGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGcacacatt                                              <  7:297269/138‑3 (MQ=255)                                                                                 ttCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGa                                       <  1:9665/138‑1 (MQ=255)                                                                                      ttCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGc                                 <  5:87644/139‑1 (MQ=255)                                                                                           cTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCctgctga                                                              <  4:154683/105‑2 (MQ=255)                                                                                           cTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCctgctga                                                              >  3:154683/1‑104 (MQ=255)                                                                                                  ggTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTcc                     <  8:97280/139‑1 (MQ=255)                                                                                                                ggCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCtt       >  1:394073/1‑139 (MQ=255)                                                                                                                      catTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACa  >  8:338961/2‑138 (MQ=255)                                                                                                                                |                                                                                                                                TTTCATTCGGATATGGATACATATCGATTCATCCTTTCTTCTAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCGATTCCGCTGGCAAGGTCAATTCCTGCCGGCTGATATTTCATTAAGTCTGTGATAGTGTCCGGATTCACGCCGCCTGCGATAAAACAACGTTTTCCTATAGCAGCCTGCTGGTATTCCGGCACACAGTCCCAAGAAAAAGCAACACCAGTGCCCCCTCTTGCCCCTTTGACA  >  CP006881/2191347‑2191601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑