Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,191,523 T→A G158G (GGA→GGT)  U712_10980 ← N‑(5'‑phosphoribosyl)anthranilate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,191,5230TA95.0% 115.9 / ‑2.1 40G158G (GGA→GGT) U712_10980N‑(5'‑phosphoribosyl)anthranilate isomerase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base A (21/17);  total (22/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

TAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCGATTCCGCTGGCAAGGTCAATTCCTGCCGGCTGATATTTCATTAAGTCTGTGATAGTGTCCGGATTCACGCCGCCTGCGATAAAACAACGTTTTCCTATAGCAGCCTGCTGGTATTCCGGCACACAGTCCCAAGAAAAAGCAACACCAGTGCCCCCTCTTGCCCCTTTGACAGATGAATCAATCACAAAGCCGTCCACATCATCTTTAAAGCGGGCTATCTCTTGAGTT  >  CP006881/2191388‑2191658
                                                                                                                                       |                                                                                                                                       
tAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTAcc                                                                                                                                       >  2:372856/1‑138 (MQ=255)
         catcaGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAgccgcc                                                                                                                             >  1:163267/1‑139 (MQ=255)
          atcaGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAgccgcc                                                                                                                             <  1:372856/138‑1 (MQ=255)
          atcaGATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGACGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAgccgcc                                                                                                                             <  8:84025/138‑1 (MQ=255)
              gATTCTGATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCctgctg                                                                                                                        <  8:375163/139‑1 (MQ=255)
                 tCTGAGCTTTTTGTCCGGTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGCTGCTTTCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATa                                                                                                                     >  3:45375/1‑139 (MQ=255)
                    gATCTTTTTGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTc                                                                                                                  >  2:9665/1‑139 (MQ=255)
                          tttGTCCGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGca                                                                                                             >  7:97280/1‑138 (MQ=255)
                                cGTTTTTTTCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGcacacatt                                                                                                       <  7:297269/138‑3 (MQ=255)
                                       ttCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGa                                                                                                <  1:9665/138‑1 (MQ=255)
                                            ttCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGc                                                                                          <  5:87644/139‑1 (MQ=255)
                                                 cTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCctgctga                                                                                                                       <  4:154683/105‑2 (MQ=255)
                                                 cTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCctgctga                                                                                                                       >  3:154683/1‑104 (MQ=255)
                                                        ggTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTcc                                                                              <  8:97280/139‑1 (MQ=255)
                                                                      ggCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCtt                                                                >  1:394073/1‑139 (MQ=255)
                                                                            catTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACa                                                           >  8:338961/2‑138 (MQ=255)
                                                                                                    tCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGt                                     <  3:212251/136‑1 (MQ=255)
                                                                                                    tCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGt                                     >  4:212251/1‑136 (MQ=255)
                                                                                                       ggATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAAcat                               <  5:179982/139‑1 (MQ=255)
                                                                                                        gATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAAcatt                              <  2:14608/139‑2 (MQ=255)
                                                                                                         aTTCACGCCGCCTGCGATAAAACAACGTTTTCCTATAGCAGCCTGCTGGTATTCCGGCACACAGTCCCAAGAAAAAGCAACACCAGTGCCCCCTCTTGCCCCTTTGACAGATGAATCAATCACAAAGCCGTCcac                                 <  5:214856/135‑1 (MQ=255)
                                                                                                         aTTCACGCCGCCTGCGATAAAACAACGTTTTCCTATAGCAGCCTGCTGGTATTCCGGAACACAGTCCCAAGAAAAAGCAACACCAGTGCCCCATCTTGCCCCTTTGACAGATGAATCAATCACAAAGCCGTCcac                                 >  6:214856/1‑135 (MQ=255)
                                                                                                          ttCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGCCCCTTTTACAGATGAATCAATCACAAAGCCGTCAAcatta                             >  6:89007/1‑136 (MQ=255)
                                                                                                                 cgccAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTaaa                     <  2:394073/139‑1 (MQ=255)
                                                                                                                       cGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGc               <  5:415524/139‑1 (MQ=255)
                                                                                                                        gATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCt              <  6:183540/139‑1 (MQ=255)
                                                                                                                                 gcGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCtt       >  1:192886/2‑137 (MQ=255)
                                                                                                                                  cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACCTTCCCAAGAAAATGCACCACCAGTTCCGCCTCTAGCCCCTTTTACAGATGAATCAATCACAAAGCCGTCAACAGTATCGTTTAAGCGGGCGATTTCTTGAg    >  4:239424/1‑139 (MQ=255)
                                                                                                                                  cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGTATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg    <  2:163267/139‑1 (MQ=255)
                                                                                                                                  cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGt                                     >  6:206111/1‑106 (MQ=255)
                                                                                                                                  cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGt                                     <  5:206111/106‑1 (MQ=255)
                                                                                                                                  cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTGTTGAg    >  5:380888/1‑139 (MQ=255)
                                                                                                                                  cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg    >  1:248384/1‑139 (MQ=255)
                                                                                                                                  cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg    >  6:360592/1‑139 (MQ=255)
                                                                                                                                  cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg    <  2:192886/139‑1 (MQ=255)
                                                                                                                                  cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg    >  7:170366/1‑139 (MQ=255)
                                                                                                                                  cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg    >  7:27908/1‑139 (MQ=255)
                                                                                                                                  cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTAACTTTAAAGCGGGCTATTTCTTGAg    >  3:377026/1‑139 (MQ=255)
                                                                                                                                  cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGAAACACCAGTTCCGCCTATAGACCCTTTTAAAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATATATTGat    >  3:223041/1‑138 (MQ=255)
                                                                                                                                  cGTTTACCAATAGCCGCCTGCTGATATTCAGGCACACATTCCCAAGAAAATGCAACACCAGT‑‑TCCCTCTAGACCATTTTACAGATGAATCAATCACAAAGCAGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGtt  >  5:13699/1‑139 (MQ=255)
                                                                                                                                    tttaCCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGt                                     <  7:382092/104‑1 (MQ=255)
                                                                                                                                    tttaCCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGt                                     >  8:382092/1‑104 (MQ=255)
                                                                                                                                    tttaCCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg    >  6:93358/1‑137 (MQ=255)
                                                                                                                                    tttaCCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg    <  5:93358/137‑1 (MQ=255)
                                                                                                                                    tttaCCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGtt  >  6:190318/1‑139 (MQ=255)
                                                                                                                                    tttaCCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGtt  >  8:41576/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                       
TAAAAGCCTCATCAGATTCTGATCTTTTTGTCCGTTTTTTTCGATTCCGCTGGCAAGGTCAATTCCTGCCGGCTGATATTTCATTAAGTCTGTGATAGTGTCCGGATTCACGCCGCCTGCGATAAAACAACGTTTTCCTATAGCAGCCTGCTGGTATTCCGGCACACAGTCCCAAGAAAAAGCAACACCAGTGCCCCCTCTTGCCCCTTTGACAGATGAATCAATCACAAAGCCGTCCACATCATCTTTAAAGCGGGCTATCTCTTGAGTT  >  CP006881/2191388‑2191658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑