Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,191,556 G→T D147E (GAC→GAA)  U712_10980 ← N‑(5'‑phosphoribosyl)anthranilate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,191,5560GT96.1% 150.5 / ‑3.6 51D147E (GAC→GAA) U712_10980N‑(5'‑phosphoribosyl)anthranilate isomerase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (25/24);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCGATTCCGCTGGCAAGGTCAATTCCTGCCGGCTGATATTTCATTAAGTCTGTGATAGTGTCCGGATTCACGCCGCCTGCGATAAAACAACGTTTTCCTATAGCAGCCTGCTGGTATTCCGGCACACAGTCCCAAGAAAAAGCAACACCAGTGCCCCCTCTTGCCCCTTTGACAGATGAATCAATCACAAAGCCGTCCACATCATCTTTAAAGCGGGCTATCTCTTGAGTTGTGTTCTCATGATGGTGAAGCGCCTTCCA  >  CP006881/2191427‑2191687
                                                                                                                                 |                                                                                                                                   
ttCAATTCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGa                                                                                                                             <  1:9665/138‑1 (MQ=255)
     ttCCGCTGGCAAGGTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGc                                                                                                                       <  5:87644/139‑1 (MQ=255)
                 ggTCAATTCCTTCTGGCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTcc                                                                                                           <  8:97280/139‑1 (MQ=255)
                               ggCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCtt                                                                                             >  1:394073/1‑139 (MQ=255)
                                     catTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACa                                                                                        >  8:338961/2‑138 (MQ=255)
                                                             tCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGt                                                                  >  4:212251/1‑136 (MQ=255)
                                                             tCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGt                                                                  <  3:212251/136‑1 (MQ=255)
                                                                ggATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAAcat                                                            <  5:179982/139‑1 (MQ=255)
                                                                 gATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAAcatt                                                           <  2:14608/139‑2 (MQ=255)
                                                                  aTTCACGCCGCCTGCGATAAAACAACGTTTTCCTATAGCAGCCTGCTGGTATTCCGGCACACAGTCCCAAGAAAAAGCAACACCAGTGCCCCCTCTTGCCCCTTTGACAGATGAATCAATCACAAAGCCGTCcac                                                              <  5:214856/135‑1 (MQ=255)
                                                                  aTTCACGCCGCCTGCGATAAAACAACGTTTTCCTATAGCAGCCTGCTGGTATTCCGGAACACAGTCCCAAGAAAAAGCAACACCAGTGCCCCATCTTGCCCCTTTGACAGATGAATCAATCACAAAGCCGTCcac                                                              >  6:214856/1‑135 (MQ=255)
                                                                   ttCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGCCCCTTTTACAGATGAATCAATCACAAAGCCGTCAAcatta                                                          >  6:89007/1‑136 (MQ=255)
                                                                          cgccAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTaaa                                                  <  2:394073/139‑1 (MQ=255)
                                                                                cGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGc                                            <  5:415524/139‑1 (MQ=255)
                                                                                 gATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCt                                           <  6:183540/139‑1 (MQ=255)
                                                                                          gcGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCtt                                    >  1:192886/2‑137 (MQ=255)
                                                                                           cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACCTTCCCAAGAAAATGCACCACCAGTTCCGCCTCTAGCCCCTTTTACAGATGAATCAATCACAAAGCCGTCAACAGTATCGTTTAAGCGGGCGATTTCTTGAg                                 >  4:239424/1‑139 (MQ=255)
                                                                                           cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGTATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                 <  2:163267/139‑1 (MQ=255)
                                                                                           cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGt                                                                  >  6:206111/1‑106 (MQ=255)
                                                                                           cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGt                                                                  <  5:206111/106‑1 (MQ=255)
                                                                                           cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTGTTGAg                                 >  5:380888/1‑139 (MQ=255)
                                                                                           cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                 >  1:248384/1‑139 (MQ=255)
                                                                                           cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                 >  6:360592/1‑139 (MQ=255)
                                                                                           cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                 >  7:170366/1‑139 (MQ=255)
                                                                                           cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                 >  7:27908/1‑139 (MQ=255)
                                                                                           cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                 <  2:192886/139‑1 (MQ=255)
                                                                                           cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTAACTTTAAAGCGGGCTATTTCTTGAg                                 >  3:377026/1‑139 (MQ=255)
                                                                                           cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGAAACACCAGTTCCGCCTATAGACCCTTTTAAAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATATATTGat                                 >  3:223041/1‑138 (MQ=255)
                                                                                           cGTTTACCAATAGCCGCCTGCTGATATTCAGGCACACATTCCCAAGAAAATGCAACACCAGT‑‑TCCCTCTAGACCATTTTACAGATGAATCAATCACAAAGCAGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGtt                               >  5:13699/1‑139 (MQ=255)
                                                                                             tttaCCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGt                                                                  >  8:382092/1‑104 (MQ=255)
                                                                                             tttaCCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGt                                                                  <  7:382092/104‑1 (MQ=255)
                                                                                             tttaCCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                 <  5:93358/137‑1 (MQ=255)
                                                                                             tttaCCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                 >  6:93358/1‑137 (MQ=255)
                                                                                             tttaCCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGtt                               >  6:190318/1‑139 (MQ=255)
                                                                                             tttaCCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGtt                               >  8:41576/1‑139 (MQ=255)
                                                                                                 cgaataGCCGCCTGCTGATATTCCGGGACACATACCCAAGAAAATGCAACACCAGATCCGCCTCTAGACCCTTTTACAGTTGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTgtgt                           <  3:426853/136‑1 (MQ=255)
                                                                                                 ccaataGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCATTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTgtgt                           <  5:190318/136‑1 (MQ=255)
                                                                                                 ccaataGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTtgtg                            <  3:169322/135‑1 (MQ=255)
                                                                                                 ccaataGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTgtgt                           <  7:41576/136‑1 (MQ=255)
                                                                                                 ccaataGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCATTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTtgtg                            <  7:338961/135‑1 (MQ=255)
                                                                                                       gccgcCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                 <  4:27867/124‑1 (MQ=255)
                                                                                                       gccgcCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                 >  3:27867/4‑127 (MQ=255)
                                                                                                        ccgcCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCttg                    >  4:245686/3‑139 (MQ=255)
                                                                                                           ccTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCttgatg                 >  7:430925/1‑139 (MQ=255)
                                                                                                           ccTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCttgatg                 >  5:261663/1‑139 (MQ=255)
                                                                                                                     ttCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGAAGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGcc       <  3:239424/139‑1 (MQ=255)
                                                                                                                          gCACACATTCCGAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGCTGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGTGTTGTGTTATCTTGATGGTGAAGCGCCTTCCa  <  4:13629/139‑1 (MQ=255)
                                                                                                                          gCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACTAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTgtgt                           <  3:302119/114‑1 (MQ=255)
                                                                                                                          gCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTcc   >  5:100033/1‑138 (MQ=255)
                                                                                                                          gCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTcc   >  8:106703/1‑138 (MQ=255)
                                                                                                                          gCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCa  <  3:245686/139‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                   
TTCGATTCCGCTGGCAAGGTCAATTCCTGCCGGCTGATATTTCATTAAGTCTGTGATAGTGTCCGGATTCACGCCGCCTGCGATAAAACAACGTTTTCCTATAGCAGCCTGCTGGTATTCCGGCACACAGTCCCAAGAAAAAGCAACACCAGTGCCCCCTCTTGCCCCTTTGACAGATGAATCAATCACAAAGCCGTCCACATCATCTTTAAAGCGGGCTATCTCTTGAGTTGTGTTCTCATGATGGTGAAGCGCCTTCCA  >  CP006881/2191427‑2191687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑