Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,191,589 T→A A136S (GCA→TCT)  U712_10980 ← N‑(5'‑phosphoribosyl)anthranilate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,191,5890TA96.5% 166.4 / ‑3.4 58A136S (GCA→TCT) U712_10980N‑(5'‑phosphoribosyl)anthranilate isomerase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base A (27/29);  total (28/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

GGCTGATATTTCATTAAGTCTGTGATAGTGTCCGGATTCACGCCGCCTGCGATAAAACAACGTTTTCCTATAGCAGCCTGCTGGTATTCCGGCACACAGTCCCAAGAAAAAGCAACACCAGTGCCCCCTCTTGCCCCTTTGACAGATGAATCAATCACAAAGCCGTCCACATCATCTTTAAAGCGGGCTATCTCTTGAGTTGTGTTCTCATGATGGTGAAGCGCCTTCCATATTTCACAATCTGTCAGCATGCGAAG  >  CP006881/2191458‑2191714
                                                                                                                                   |                                                                                                                             
ggCTGCCATTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCtt                                                                                                                        >  1:394073/1‑139 (MQ=255)
      catTTCAATAGGCGTGTGATGCTATCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACa                                                                                                                   >  8:338961/2‑138 (MQ=255)
                              tCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGt                                                                                             >  4:212251/1‑136 (MQ=255)
                              tCCGGATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGt                                                                                             <  3:212251/136‑1 (MQ=255)
                                 ggATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAAcat                                                                                       <  5:179982/139‑1 (MQ=255)
                                  gATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAAcatt                                                                                      <  2:14608/139‑2 (MQ=255)
                                   aTTCACGCCGCCTGCGATAAAACAACGTTTTCCTATAGCAGCCTGCTGGTATTCCGGCACACAGTCCCAAGAAAAAGCAACACCAGTGCCCCCTCTTGCCCCTTTGACAGATGAATCAATCACAAAGCCGTCcac                                                                                         <  5:214856/135‑1 (MQ=255)
                                   aTTCACGCCGCCTGCGATAAAACAACGTTTTCCTATAGCAGCCTGCTGGTATTCCGGAACACAGTCCCAAGAAAAAGCAACACCAGTGCCCCATCTTGCCCCTTTGACAGATGAATCAATCACAAAGCCGTCcac                                                                                         >  6:214856/1‑135 (MQ=255)
                                    ttCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGCCCCTTTTACAGATGAATCAATCACAAAGCCGTCAAcatta                                                                                     >  6:89007/1‑136 (MQ=255)
                                           cgccAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTaaa                                                                             <  2:394073/139‑1 (MQ=255)
                                                 cGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGc                                                                       <  5:415524/139‑1 (MQ=255)
                                                  gATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCt                                                                      <  6:183540/139‑1 (MQ=255)
                                                           gcGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCtt                                                               >  1:192886/2‑137 (MQ=255)
                                                            cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACCTTCCCAAGAAAATGCACCACCAGTTCCGCCTCTAGCCCCTTTTACAGATGAATCAATCACAAAGCCGTCAACAGTATCGTTTAAGCGGGCGATTTCTTGAg                                                            >  4:239424/1‑139 (MQ=255)
                                                            cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGTATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                                            <  2:163267/139‑1 (MQ=255)
                                                            cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGt                                                                                             >  6:206111/1‑106 (MQ=255)
                                                            cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGt                                                                                             <  5:206111/106‑1 (MQ=255)
                                                            cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTGTTGAg                                                            >  5:380888/1‑139 (MQ=255)
                                                            cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                                            >  1:248384/1‑139 (MQ=255)
                                                            cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                                            >  7:27908/1‑139 (MQ=255)
                                                            cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                                            >  7:170366/1‑139 (MQ=255)
                                                            cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                                            >  6:360592/1‑139 (MQ=255)
                                                            cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                                            <  2:192886/139‑1 (MQ=255)
                                                            cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTAACTTTAAAGCGGGCTATTTCTTGAg                                                            >  3:377026/1‑139 (MQ=255)
                                                            cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGAAACACCAGTTCCGCCTATAGACCCTTTTAAAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATATATTGat                                                            >  3:223041/1‑138 (MQ=255)
                                                            cGTTTACCAATAGCCGCCTGCTGATATTCAGGCACACATTCCCAAGAAAATGCAACACCAGT‑‑TCCCTCTAGACCATTTTACAGATGAATCAATCACAAAGCAGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGtt                                                          >  5:13699/1‑139 (MQ=255)
                                                              tttaCCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGt                                                                                             <  7:382092/104‑1 (MQ=255)
                                                              tttaCCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGt                                                                                             >  8:382092/1‑104 (MQ=255)
                                                              tttaCCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                                            >  6:93358/1‑137 (MQ=255)
                                                              tttaCCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                                            <  5:93358/137‑1 (MQ=255)
                                                              tttaCCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGtt                                                          >  8:41576/1‑139 (MQ=255)
                                                              tttaCCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGtt                                                          >  6:190318/1‑139 (MQ=255)
                                                                  cgaataGCCGCCTGCTGATATTCCGGGACACATACCCAAGAAAATGCAACACCAGATCCGCCTCTAGACCCTTTTACAGTTGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTgtgt                                                      <  3:426853/136‑1 (MQ=255)
                                                                  ccaataGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCATTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTgtgt                                                      <  5:190318/136‑1 (MQ=255)
                                                                  ccaataGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTtgtg                                                       <  3:169322/135‑1 (MQ=255)
                                                                  ccaataGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTgtgt                                                      <  7:41576/136‑1 (MQ=255)
                                                                  ccaataGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCATTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTtgtg                                                       <  7:338961/135‑1 (MQ=255)
                                                                        gccgcCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                                            >  3:27867/4‑127 (MQ=255)
                                                                        gccgcCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                                            <  4:27867/124‑1 (MQ=255)
                                                                         ccgcCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCttg                                               >  4:245686/3‑139 (MQ=255)
                                                                            ccTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCttgatg                                            >  5:261663/1‑139 (MQ=255)
                                                                            ccTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCttgatg                                            >  7:430925/1‑139 (MQ=255)
                                                                                      ttCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGAAGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGcc                                  <  3:239424/139‑1 (MQ=255)
                                                                                           gCACACATTCCGAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGCTGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGTGTTGTGTTATCTTGATGGTGAAGCGCCTTCCa                             <  4:13629/139‑1 (MQ=255)
                                                                                           gCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACTAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTgtgt                                                      <  3:302119/114‑1 (MQ=255)
                                                                                           gCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTcc                              >  8:106703/1‑138 (MQ=255)
                                                                                           gCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTcc                              >  5:100033/1‑138 (MQ=255)
                                                                                           gCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCa                             <  3:245686/139‑1 (MQ=255)
                                                                                                 attCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGccgt                                >  7:380700/3‑128 (MQ=255)
                                                                                                 attCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCtt                                <  8:380700/128‑1 (MQ=255)
                                                                                                  ttCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTca                      <  7:106703/138‑1 (MQ=255)
                                                                                                  ttCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTca                      <  8:27908/138‑1 (MQ=255)
                                                                                                    cccAAGAAAAAGCAACACCAGGTCCGCCTCTAGACCCGTTAACAGCTGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTcac                     <  6:380888/138‑1 (MQ=255)
                                                                                                                   cacCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                                            <  5:325851/84‑1 (MQ=255)
                                                                                                                   cacCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                                            >  6:325851/1‑84 (MQ=255)
                                                                                                                   cacCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCg     <  5:360592/139‑1 (MQ=255)
                                                                                                                   cacCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCg     <  8:430925/139‑1 (MQ=255)
                                                                                                                      cAGTTCCGCCTCTAGACCCTTTTACAGATGAATGAATCACAAAGCGGTCAACATTATGTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGTTGGTGAAGCGCCTTCCATATTTCTCAGCCTGTCAGCTTGCGTAg  <  4:223041/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                             
GGCTGATATTTCATTAAGTCTGTGATAGTGTCCGGATTCACGCCGCCTGCGATAAAACAACGTTTTCCTATAGCAGCCTGCTGGTATTCCGGCACACAGTCCCAAGAAAAAGCAACACCAGTGCCCCCTCTTGCCCCTTTGACAGATGAATCAATCACAAAGCCGTCCACATCATCTTTAAAGCGGGCTATCTCTTGAGTTGTGTTCTCATGATGGTGAAGCGCCTTCCATATTTCACAATCTGTCAGCATGCGAAG  >  CP006881/2191458‑2191714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑