Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,173,484 A→G Y88Y (TAT→TAC)  U712_10870 ← Bifunctional protein BirA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,173,4840AG100.0% 116.9 / NA 37Y88Y (TAT→TAC) U712_10870Bifunctional protein BirA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/21);  total (16/21)

GAGAATGCCATACCCTAGACATTCGACCTCTGCCGGCTGTTTGTTTGTCAGCCACCACAAGAGTGCCTTCCGGTGCGTTATTATTCGCAAGCTCATGCGCCGTTTTTTGCGTGCTTGAAATAACGTCTTGATAAATAAGATGCTGGCCCATTACTTCCGTTTTCAATCCGAAGCGAATTTCACTTTCACTGAGTTTCCCGGGCTTTTTGATAAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAGCCCTCTTTCCGA  >  CP006881/2173354‑2173616
                                                                                                                                  |                                                                                                                                    
gagaATGCCATACCCTCGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGgtaaata                                                                                                                                <  2:370288/137‑1 (MQ=255)
gagaATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGGGTGCTTGAAAGAACGTCATGgtaaat                                                                                                                                 >  3:93439/1‑136 (MQ=255)
     tGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGgtaaata                                                                                                                                >  3:196399/1‑132 (MQ=255)
     tGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGgtaaata                                                                                                                                <  4:196399/132‑1 (MQ=255)
            cccTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGTACGTCATGGTAAATAAGATGCTGGCCCAt                                                                                                                  <  1:98030/139‑1 (MQ=255)
               tAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAATAACGTCATGGTAAATAAGATGCTGGCCCagcac                                                                                                               >  4:378553/1‑135 (MQ=255)
               tAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCAc                                                                                                               <  6:69217/139‑1 (MQ=255)
                      tCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGtt                                                                                                        >  7:2745/1‑139 (MQ=255)
                      tCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTGGTTTGGGTGCGTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTACGtt                                                                                                        >  4:24353/1‑139 (MQ=255)
                              ttccGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATc                                                                                                 >  5:182885/3‑138 (MQ=255)
                              ttccGGCTGGTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGAGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTTCTTTTAATCACGGGATGGGAAATAATATTCTGGCCCATAACTTCCGTGTTTAAta                                                                                                 >  7:126247/3‑137 (MQ=255)
                                                   ccaccaCAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATttcgctttcact                                                                           <  6:182885/139‑1 (MQ=255)
                                                             ggTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAAtt                                                                                      >  8:94194/2‑118 (MQ=255)
                                                             ggTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAAtt                                                                                      <  7:94194/117‑1 (MQ=255)
                                                             ggTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTGTTTTGGGTGCTTGAAAGAACGTCATGGTAAATAAGATTCTGGCCCATCACTTCCGTTTTTAATACAAAAAGAATTTCGCTTTCACTGAGttttgcg                                                                 >  3:268365/2‑135 (MQ=255)
                                                                 ccTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCgggtt                                                             >  6:71674/1‑137 (MQ=255)
                                                                 ccTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTGTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGGTTGCCCATCACTTCCGTTTTTTATGGAAAAAGAATTTTGAGTTCAATGAGTTTTCCggggg                                                             >  4:43951/1‑137 (MQ=255)
                                                                 ccTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTGTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGGTGGCCCCTCACTTCCGTTTTTTATCCAAACAGAATTTCGCATTCACTGAGTTTTACgggtt                                                             >  8:111011/1‑137 (MQ=255)
                                                                      cGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTg                                                        <  5:284325/139‑1 (MQ=255)
                                                                        gTGCGTTATTATTCGCGAGCTCATGATCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGATTTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGAt                                                      <  3:26390/139‑1 (MQ=255)
                                                                         tGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGAtg                                                     <  8:2745/139‑2 (MQ=255)
                                                                           cGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAg                                                   <  6:16658/139‑1 (MQ=255)
                                                                                  attCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGata                                             <  5:385419/138‑1 (MQ=255)
                                                                                     cgcgAGCTCATGAGCCGTTTTTTGCGTGCTTGAAATAACGTCATGgtaaata                                                                                                                                >  6:197633/1‑52 (MQ=255)
                                                                                     cgcgAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGgtaaata                                                                                                                                <  5:197633/52‑1 (MQ=255)
                                                                                             cATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATGAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTttcttc                                  >  3:128616/1‑138 (MQ=255)
                                                                                              aTGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTa                                <  1:158952/139‑1 (MQ=255)
                                                                                              aTGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTa                                <  3:146728/139‑1 (MQ=255)
                                                                                              aTGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTa                                <  5:71674/139‑1 (MQ=255)
                                                                                              aTGAGCCGTTTTTTGCGCGCTTGAAAGAACGTCATGGTAAATAAGATGCGGGCCCCTCACTTCCGTCTTTAATCCAAAACGAATTTCGCTTTCACTGAGGTTTCCGGGTGTTTTGATGAGCCGATATCCTTTTCTTCTa                                <  8:44210/139‑1 (MQ=255)
                                                                                                      ttttttGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTGGGTTTCAATGAGTTTTACGGGTTTTTTTATGAGCCGATATCCTTTTATTATAACGGCTTc                        >  6:143087/1‑139 (MQ=255)
                                                                                                      ttttttGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTc                        <  4:93439/139‑1 (MQ=255)
                                                                                                         tttGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTa                      <  1:246915/138‑1 (MQ=255)
                                                                                                             cGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCa                 >  7:68288/1‑139 (MQ=255)
                                                                                                                gCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTa                                <  2:85996/121‑1 (MQ=255)
                                                                                                                gCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTa                                >  1:85996/1‑121 (MQ=255)
                                                                                                                 cTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTc                        <  6:75035/128‑1 (MQ=255)
                                                                                                                 cTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTc                        >  5:75035/1‑128 (MQ=255)
                                                                                                                     aaaGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCct          <  2:106015/138‑1 (MQ=255)
                                                                                                                     aaaGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCct          <  7:77809/138‑1 (MQ=255)
                                                                                                                           cGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCg   >  3:79947/1‑139 (MQ=255)
                                                                                                                           cGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCg   <  3:170857/139‑1 (MQ=255)
                                                                                                                            gTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGa  <  2:58054/139‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                    
GAGAATGCCATACCCTAGACATTCGACCTCTGCCGGCTGTTTGTTTGTCAGCCACCACAAGAGTGCCTTCCGGTGCGTTATTATTCGCAAGCTCATGCGCCGTTTTTTGCGTGCTTGAAATAACGTCTTGATAAATAAGATGCTGGCCCATTACTTCCGTTTTCAATCCGAAGCGAATTTCACTTTCACTGAGTTTCCCGGGCTTTTTGATAAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAGCCCTCTTTCCGA  >  CP006881/2173354‑2173616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑