Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,191,625	C→A	V124V (GTG→GTT)	U712_10980 ←	N‑(5'‑phosphoribosyl)anthranilate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,191,625	0	C	A	100.0%	190.0 / NA	62	V124V (GTG→GTT)	U712_10980	N‑(5'‑phosphoribosyl)anthranilate isomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (31/31);  total (31/31)

GGATTCACGCCGCCTGCGATAAAACAACGTTTTCCTATAGCAGCCTGCTGGTATTCCGGCACACAGTCCCAAGAAAAAGCAACACCAGTGCCCCCTCTTGCCCCTTTGACAGATGAATCAATCACAAAGCCGTCCACATCATCTTTAAAGCGGGCTATCTCTTGAGTTGTGTTCTCATGATGGTGAAGCGCCTTCCATATTTCACAATCTGTCAGCATGCGAAGCGCATCGGCATCCGCAGGTTCCTCATCACCGTGAAGCTGAATGACG  >  CP006881/2191491‑2191760                                                                                                                                       |                                                                                                                                        ggATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAAcat                                                                                                                                     <  5:179982/139‑1 (MQ=255)  gATTCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAAcatt                                                                                                                                    <  2:14608/139‑2 (MQ=255)   aTTCACGCCGCCTGCGATAAAACAACGTTTTCCTATAGCAGCCTGCTGGTATTCCGGCACACAGTCCCAAGAAAAAGCAACACCAGTGCCCCCTCTTGCCCCTTTGACAGATGAATCAATCACAAAGCCGTCcac                                                                                                                                       <  5:214856/135‑1 (MQ=255)   aTTCACGCCGCCTGCGATAAAACAACGTTTTCCTATAGCAGCCTGCTGGTATTCCGGAACACAGTCCCAAGAAAAAGCAACACCAGTGCCCCATCTTGCCCCTTTGACAGATGAATCAATCACAAAGCCGTCcac                                                                                                                                       >  6:214856/1‑135 (MQ=255)    ttCACGCCGCCAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGCCCCTTTTACAGATGAATCAATCACAAAGCCGTCAAcatta                                                                                                                                   >  6:89007/1‑136 (MQ=255)           cgccAGCGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTaaa                                                                                                                           <  2:394073/139‑1 (MQ=255)                 cGATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGc                                                                                                                     <  5:415524/139‑1 (MQ=255)                  gATAAAGCAGCGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCt                                                                                                                    <  6:183540/139‑1 (MQ=255)                           gcGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCtt                                                                                                             >  1:192886/2‑137 (MQ=255)                            cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACCTTCCCAAGAAAATGCACCACCAGTTCCGCCTCTAGCCCCTTTTACAGATGAATCAATCACAAAGCCGTCAACAGTATCGTTTAAGCGGGCGATTTCTTGAg                                                                                                          >  4:239424/1‑139 (MQ=255)                            cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGTATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                                                                                          <  2:163267/139‑1 (MQ=255)                            cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTGTTGAg                                                                                                          >  5:380888/1‑139 (MQ=255)                            cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                                                                                          >  1:248384/1‑139 (MQ=255)                            cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                                                                                          >  7:27908/1‑139 (MQ=255)                            cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                                                                                          >  7:170366/1‑139 (MQ=255)                            cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                                                                                          >  6:360592/1‑139 (MQ=255)                            cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                                                                                          <  2:192886/139‑1 (MQ=255)                            cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTAACTTTAAAGCGGGCTATTTCTTGAg                                                                                                          >  3:377026/1‑139 (MQ=255)                            cGTTTACCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGAAACACCAGTTCCGCCTATAGACCCTTTTAAAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATATATTGat                                                                                                          >  3:223041/1‑138 (MQ=255)                            cGTTTACCAATAGCCGCCTGCTGATATTCAGGCACACATTCCCAAGAAAATGCAACACCAGT‑‑TCCCTCTAGACCATTTTACAGATGAATCAATCACAAAGCAGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGtt                                                                                                        >  5:13699/1‑139 (MQ=255)                              tttaCCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                                                                                          <  5:93358/137‑1 (MQ=255)                              tttaCCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                                                                                          >  6:93358/1‑137 (MQ=255)                              tttaCCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGtt                                                                                                        >  8:41576/1‑139 (MQ=255)                              tttaCCAATAGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGtt                                                                                                        >  6:190318/1‑139 (MQ=255)                                  cgaataGCCGCCTGCTGATATTCCGGGACACATACCCAAGAAAATGCAACACCAGATCCGCCTCTAGACCCTTTTACAGTTGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTgtgt                                                                                                    <  3:426853/136‑1 (MQ=255)                                  ccaataGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCATTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTgtgt                                                                                                    <  5:190318/136‑1 (MQ=255)                                  ccaataGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTtgtg                                                                                                     <  3:169322/135‑1 (MQ=255)                                  ccaataGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTgtgt                                                                                                    <  7:41576/136‑1 (MQ=255)                                  ccaataGCCGCCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCATTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTtgtg                                                                                                     <  7:338961/135‑1 (MQ=255)                                        gccgcCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                                                                                          >  3:27867/4‑127 (MQ=255)                                        gccgcCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                                                                                          <  4:27867/124‑1 (MQ=255)                                         ccgcCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCttg                                                                                             >  4:245686/3‑139 (MQ=255)                                            ccTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCttgatg                                                                                          >  5:261663/1‑139 (MQ=255)                                            ccTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCttgatg                                                                                          >  7:430925/1‑139 (MQ=255)                                                      ttCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGAAGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGcc                                                                                <  3:239424/139‑1 (MQ=255)                                                           gCACACATTCCGAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGCTGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGTGTTGTGTTATCTTGATGGTGAAGCGCCTTCCa                                                                           <  4:13629/139‑1 (MQ=255)                                                           gCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACTAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTgtgt                                                                                                    <  3:302119/114‑1 (MQ=255)                                                           gCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTcc                                                                            >  8:106703/1‑138 (MQ=255)                                                           gCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTcc                                                                            >  5:100033/1‑138 (MQ=255)                                                           gCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCa                                                                           <  3:245686/139‑1 (MQ=255)                                                                 attCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGccgt                                                                              >  7:380700/3‑128 (MQ=255)                                                                 attCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCtt                                                                              <  8:380700/128‑1 (MQ=255)                                                                  ttCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTca                                                                    <  8:27908/138‑1 (MQ=255)                                                                  ttCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTca                                                                    <  7:106703/138‑1 (MQ=255)                                                                    cccAAGAAAAAGCAACACCAGGTCCGCCTCTAGACCCGTTAACAGCTGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTcac                                                                   <  6:380888/138‑1 (MQ=255)                                                                                   cacCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                                                                                          >  6:325851/1‑84 (MQ=255)                                                                                   cacCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                                                                                          <  5:325851/84‑1 (MQ=255)                                                                                   cacCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCg                                                   <  8:430925/139‑1 (MQ=255)                                                                                   cacCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCg                                                   <  5:360592/139‑1 (MQ=255)                                                                                      cAGTTCCGCCTCTAGACCCTTTTACAGATGAATGAATCACAAAGCGGTCAACATTATGTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGTTGGTGAAGCGCCTTCCATATTTCTCAGCCTGTCAGCTTGCGTAg                                                <  4:223041/139‑1 (MQ=255)                                                                                                         ttttACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGt                             >  7:222984/1‑139 (MQ=255)                                                                                                         ttttACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGt                             >  1:263368/1‑139 (MQ=255)                                                                                                         ttttACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGt                             >  3:295473/1‑139 (MQ=255)                                                                                                           ttaCAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCgggtt                           >  5:52877/3‑136 (MQ=255)                                                                                                                gATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTtcat                      <  6:261663/139‑1 (MQ=255)                                                                                                                 aTGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                                                                                          <  7:321043/54‑1 (MQ=255)                                                                                                                 aTGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAg                                                                                                          >  8:321043/1‑54 (MQ=255)                                                                                                                   gAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCAc                   >  5:424540/1‑139 (MQ=255)                                                                                                                        aatcaCAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTg               <  6:100033/138‑1 (MQ=255)                                                                                                                          tcaCAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAg            <  6:195946/139‑1 (MQ=255)                                                                                                                           cacaAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGc           >  4:47584/1‑139 (MQ=255)                                                                                                                               aaGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGaa       <  7:48344/139‑1 (MQ=255)                                                                                                                                aGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATAACCGTGAAGCTGAAt      >  1:413446/1‑139 (MQ=255)                                                                                                                                    gTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACg  >  6:282636/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                        GGATTCACGCCGCCTGCGATAAAACAACGTTTTCCTATAGCAGCCTGCTGGTATTCCGGCACACAGTCCCAAGAAAAAGCAACACCAGTGCCCCCTCTTGCCCCTTTGACAGATGAATCAATCACAAAGCCGTCCACATCATCTTTAAAGCGGGCTATCTCTTGAGTTGTGTTCTCATGATGGTGAAGCGCCTTCCATATTTCACAATCTGTCAGCATGCGAAGCGCATCGGCATCCGCAGGTTCCTCATCACCGTGAAGCTGAATGACG  >  CP006881/2191491‑2191760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑