Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,191,664	C→A	E111D (GAG→GAT)	U712_10980 ←	N‑(5'‑phosphoribosyl)anthranilate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,191,664	0	C	A	100.0%	135.7 / NA	45	E111D (GAG→GAT)	U712_10980	N‑(5'‑phosphoribosyl)anthranilate isomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (22/23);  total (22/23)

CAGCCTGCTGGTATTCCGGCACACAGTCCCAAGAAAAAGCAACACCAGTGCCCCCTCTTGCCCCTTTGACAGATGAATCAATCACAAAGCCGTCCACATCATCTTTAAAGCGGGCTATCTCTTGAGTTGTGTTCTCATGATGGTGAAGCGCCTTCCATATTTCACAATCTGTCAGCATGCGAAGCGCATCGGCATCCGCAGGTTCCTCATCACCGTGAAGCTGAATGACGTCTAGATTTAAG‑TCTTCGGCAATCCGTGACATCGTCTC  >  CP006881/2191531‑2191798                                                                                                                                      |                                                                                                                                        ccgcCTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCttg                                                                                                                                    >  4:245686/3‑139 (MQ=255)    ccTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCttgatg                                                                                                                                 >  7:430925/1‑139 (MQ=255)    ccTGCTGATATTCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCttgatg                                                                                                                                 >  5:261663/1‑139 (MQ=255)              ttCCGGCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGAAGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGcc                                                                                                                       <  3:239424/139‑1 (MQ=255)                   gCACACATTCCGAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGCTGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGTGTTGTGTTATCTTGATGGTGAAGCGCCTTCCa                                                                                                                  <  4:13629/139‑1 (MQ=255)                   gCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTcc                                                                                                                   >  8:106703/1‑138 (MQ=255)                   gCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTcc                                                                                                                   >  5:100033/1‑138 (MQ=255)                   gCACACATTCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCa                                                                                                                  <  3:245686/139‑1 (MQ=255)                         attCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGccgt                                                                                                                     >  7:380700/3‑128 (MQ=255)                         attCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCtt                                                                                                                     <  8:380700/128‑1 (MQ=255)                          ttCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTca                                                                                                           <  8:27908/138‑1 (MQ=255)                          ttCCCAAGAAAATGCAACACCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTca                                                                                                           <  7:106703/138‑1 (MQ=255)                            cccAAGAAAAAGCAACACCAGGTCCGCCTCTAGACCCGTTAACAGCTGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTcac                                                                                                          <  6:380888/138‑1 (MQ=255)                                           cacCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCg                                                                                          <  8:430925/139‑1 (MQ=255)                                           cacCAGTTCCGCCTCTAGACCCTTTTACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCg                                                                                          <  5:360592/139‑1 (MQ=255)                                              cAGTTCCGCCTCTAGACCCTTTTACAGATGAATGAATCACAAAGCGGTCAACATTATGTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGTTGGTGAAGCGCCTTCCATATTTCTCAGCCTGTCAGCTTGCGTAg                                                                                       <  4:223041/139‑1 (MQ=255)                                                                 ttttACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGt                                                                    >  1:263368/1‑139 (MQ=255)                                                                 ttttACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGt                                                                    >  3:295473/1‑139 (MQ=255)                                                                 ttttACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGt                                                                    >  7:222984/1‑139 (MQ=255)                                                                   ttaCAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCgggtt                                                                  >  5:52877/3‑136 (MQ=255)                                                                        gATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTtcat                                                             <  6:261663/139‑1 (MQ=255)                                                                           gAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCAc                                                          >  5:424540/1‑139 (MQ=255)                                                                                aatcaCAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTg                                                      <  6:100033/138‑1 (MQ=255)                                                                                  tcaCAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAg                                                   <  6:195946/139‑1 (MQ=255)                                                                                   cacaAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGc                                                  >  4:47584/1‑139 (MQ=255)                                                                                       aaGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGaa                                              <  7:48344/139‑1 (MQ=255)                                                                                        aGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATAACCGTGAAGCTGAAt                                             >  1:413446/1‑139 (MQ=255)                                                                                            gTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACg                                         >  6:282636/1‑139 (MQ=255)                                                                                              caacaTTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTc                                       <  6:424540/137‑1 (MQ=255)                                                                                                    tatcTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCtt                                 >  1:348446/2‑139 (MQ=255)                                                                                                    tatatttAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGGCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAgct                                  >  2:285180/5‑136 (MQ=255)                                                                                                      tcTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCttc                                <  3:47584/138‑2 (MQ=255)                                                                                                           aaaGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCGTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACTTCGAGCTTCAAgct                           >  2:157108/1‑136 (MQ=255)                                                                                                                  gCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTcg                                      <  4:183476/120‑2 (MQ=255)                                                                                                                  gCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTcg                                      >  3:183476/1‑119 (MQ=255)                                                                                                                      tttctTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAAtac               <  4:295473/136‑3 (MQ=255)                                                                                                                           tGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCg                                                                                          <  5:4448/59‑1 (MQ=255)                                                                                                                           tGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCg                                                                                          >  6:4448/1‑59 (MQ=255)                                                                                                                             aGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACAt        <  2:348446/138‑1 (MQ=255)                                                                                                                             aGTGGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACAt        <  4:377026/138‑1 (MQ=255)                                                                                                                               ttGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGt     <  6:52877/139‑1 (MQ=255)                                                                                                                                  tgtTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACAt        <  1:258117/133‑1 (MQ=255)                                                                                                                                  tgtTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACAt        >  2:258117/1‑133 (MQ=255)                                                                                                                                  tgtTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGtctc  >  7:315092/1‑139 (MQ=255)                                                                                                                                  tgtTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGtctc  >  2:7885/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                        CAGCCTGCTGGTATTCCGGCACACAGTCCCAAGAAAAAGCAACACCAGTGCCCCCTCTTGCCCCTTTGACAGATGAATCAATCACAAAGCCGTCCACATCATCTTTAAAGCGGGCTATCTCTTGAGTTGTGTTCTCATGATGGTGAAGCGCCTTCCATATTTCACAATCTGTCAGCATGCGAAGCGCATCGGCATCCGCAGGTTCCTCATCACCGTGAAGCTGAATGACGTCTAGATTTAAG‑TCTTCGGCAATCCGTGACATCGTCTC  >  CP006881/2191531‑2191798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑