Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,191,730	A→C	P89P (CCT→CCG)	U712_10980 ←	N‑(5'‑phosphoribosyl)anthranilate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,191,730	0	A	C	100.0%	181.8 / NA	56	P89P (CCT→CCG)	U712_10980	N‑(5'‑phosphoribosyl)anthranilate isomerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (35/21);  total (35/21)

TTTGACAGATGAATCAATCACAAAGCCGTCCACATCATCTTTAAAGCGGGCTATCTCTTGAGTTGTGTTCTCATGATGGTGAAGCGCCTTCCATATTTCACAATCTGTCAGCATGCGAAGCGCATCGGCATCCGCAGGTTCCTCATCACCGTGAAGCTGAATGACGTCTAGATTTAAG‑TCTTCGGCAATCCGTGACATCGTCTCTATCGATTCATTAACAAAAACCCCTGCAACCTGTTTTTCGACACAAACTTGACTCAGCCACTTTTTC  >  CP006881/2191595‑2191865                                                                                                                                        |                                                                                                                                         ttttACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGt                                                                                                                                       >  7:222984/1‑139 (MQ=255) ttttACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGt                                                                                                                                       >  1:263368/1‑139 (MQ=255) ttttACAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGt                                                                                                                                       >  3:295473/1‑139 (MQ=255)   ttaCAGATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCgggtt                                                                                                                                     >  5:52877/3‑136 (MQ=255)        gATGAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTtcat                                                                                                                                <  6:261663/139‑1 (MQ=255)           gAATCAATCACAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCAc                                                                                                                             >  5:424540/1‑139 (MQ=255)                aatcaCAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTg                                                                                                                         <  6:100033/138‑1 (MQ=255)                  tcaCAAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAg                                                                                                                      <  6:195946/139‑1 (MQ=255)                   cacaAAGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGc                                                                                                                     >  4:47584/1‑139 (MQ=255)                       aaGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGaa                                                                                                                 <  7:48344/139‑1 (MQ=255)                        aGCCGTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATAACCGTGAAGCTGAAt                                                                                                                >  1:413446/1‑139 (MQ=255)                            gTCAACATTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACg                                                                                                            >  6:282636/1‑139 (MQ=255)                              caacaTTATCTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTc                                                                                                          <  6:424540/137‑1 (MQ=255)                                    tatcTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCtt                                                                                                    >  1:348446/2‑139 (MQ=255)                                    tatatttAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGGCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAgct                                                                                                     >  2:285180/5‑136 (MQ=255)                                      tcTTTAAAGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCttc                                                                                                   <  3:47584/138‑2 (MQ=255)                                           aaaGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCGTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACTTCGAGCTTCAAgct                                                                                              >  2:157108/1‑136 (MQ=255)                                                  gCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTcg                                                                                                         >  3:183476/1‑119 (MQ=255)                                                  gCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTcg                                                                                                         <  4:183476/120‑2 (MQ=255)                                                      tttctTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAAtac                                                                                  <  4:295473/136‑3 (MQ=255)                                                             aGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACAt                                                                           <  2:348446/138‑1 (MQ=255)                                                             aGTGGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACAt                                                                           <  4:377026/138‑1 (MQ=255)                                                               ttGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGt                                                                        <  6:52877/139‑1 (MQ=255)                                                                  tgtTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACAt                                                                           <  1:258117/133‑1 (MQ=255)                                                                  tgtTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACAt                                                                           >  2:258117/1‑133 (MQ=255)                                                                  tgtTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGtctc                                                                     >  7:315092/1‑139 (MQ=255)                                                                  tgtTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGtctc                                                                     >  2:7885/1‑139 (MQ=255)                                                                       tcttgatgGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTAttg                                                                >  7:242276/4‑137 (MQ=255)                                                                            atgGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATATGTGACATCGTCTCTATTGATTCa                                                           >  7:212658/1‑139 (MQ=255)                                                                            atgGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCa                                                           >  7:302110/1‑139 (MQ=255)                                                                            atgGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCa                                                           >  7:49407/1‑139 (MQ=255)                                                                                 gAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGCATGACGTCGAGCTTCACGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTaa                                                       >  2:153766/1‑138 (MQ=255)                                                                                 gAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTaa                                                       >  5:240122/1‑138 (MQ=255)                                                                                  acgcgcCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGCGCAGCAGCATCCGCCGGTTTTTCCTCCCCGTGAAGATGAATGACGTAGCGCTTCAAGCTCGT‑GTCAATACGTGACTTCGTCTCTATTTATTCAATAAc                                                      >  4:236920/3‑138 (MQ=255)                                                                                  aaGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATAACCGTGAAGCTGAATGACGTCGAGATTCAAGCTCTT‑GGCAATACGTGACATCGACTCTATTGATTCAATAAc                                                      >  8:6557/1‑138 (MQ=255)                                                                                          tCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAAcacc                                             >  5:118448/1‑139 (MQ=255)                                                                                                 ttCACAGCCTGTCAGCTTGCGAAGCGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATGCATTAACAAAAACACCGGCAAcc                                      >  7:397797/1‑139 (MQ=255)                                                                                                      agccTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAAc                                       <  8:140074/130‑1 (MQ=255)                                                                                                      agccTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATAACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAAc                                       >  7:140074/4‑133 (MQ=255)                                                                                                        ccTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCttc                                                                                                   >  5:281143/2‑71 (MQ=255)                                                                                                        ccTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCttc                                                                                                   <  6:281143/71‑2 (MQ=255)                                                                                                          tGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACTGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTc                              >  5:335189/1‑138 (MQ=255)                                                                                                          tGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTc                              >  7:391604/1‑138 (MQ=255)                                                                                                           gTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGttttt                               >  6:123200/1‑136 (MQ=255)                                                                                                           gTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGttttt                               <  5:123200/136‑1 (MQ=255)                                                                                                           gTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGa                            >  1:229942/1‑139 (MQ=255)                                                                                                            tCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAGGCTCTT‑GGCATTACGTGACATCGTCTCGATTGATTCATTAGCAAAAACACCTGCACCCTGTTTTTCGac                           >  2:287172/1‑139 (MQ=255)                                                                                                            tCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGa                            <  2:413446/138‑1 (MQ=255)                                                                                                            tCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTT‑TTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGa                            <  8:170366/137‑1 (MQ=255)                                                                                                            tCAGCTTGCGAACAGCAGCAGCATCCGCCTGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGa                            <  6:13699/138‑1 (MQ=255)                                                                                                              aGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGATGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGacac                         <  1:157108/139‑1 (MQ=255)                                                                                                              aGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGacac                         <  8:391604/139‑1 (MQ=255)                                                                                                                                   tCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATtttt    >  4:370674/1‑139 (MQ=255)                                                                                                                                   tCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATtttt    >  1:250719/1‑139 (MQ=255)                                                                                                                                   tCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATtttt    >  1:226304/1‑139 (MQ=255)                                                                                                                                     cGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTc  <  6:118448/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         TTTGACAGATGAATCAATCACAAAGCCGTCCACATCATCTTTAAAGCGGGCTATCTCTTGAGTTGTGTTCTCATGATGGTGAAGCGCCTTCCATATTTCACAATCTGTCAGCATGCGAAGCGCATCGGCATCCGCAGGTTCCTCATCACCGTGAAGCTGAATGACGTCTAGATTTAAG‑TCTTCGGCAATCCGTGACATCGTCTCTATCGATTCATTAACAAAAACCCCTGCAACCTGTTTTTCGACACAAACTTGACTCAGCCACTTTTTC  >  CP006881/2191595‑2191865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑