Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,191,802 C→T S65S (TCG→TCA)  U712_10980 ← N‑(5'‑phosphoribosyl)anthranilate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,191,8020CT97.6% 120.9 / ‑4.4 42S65S (TCG→TCA) U712_10980N‑(5'‑phosphoribosyl)anthranilate isomerase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (28/13);  total (29/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.86e-01

ATGGTGAAGCGCCTTCCATATTTCACAATCTGTCAGCATGCGAAGCGCATCGGCATCCGCAGGTTCCTCATCACCGTGAAGCTGAATGACGTCTAGATTTAAG‑TCTTCGGCAATCCGTGACATCGTCTCTATCGATTCATTAACAAAAACCCCTGCAACCTGTTTTTCGACACAAACTTGACTCAGCCACTTTTTCACATCTTGCGGAGATACTTTTCGGTAGCTTTCAGCAAAAATAAAGCCCAGATAATCCGCCTGTGAG  >  CP006881/2191670‑2191931
                                                                                                                                     |                                                                                                                                 
atgGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATATGTGACATCGTCTCTATTGATTCa                                                                                                                             >  7:212658/1‑139 (MQ=255)
atgGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCa                                                                                                                             >  7:302110/1‑139 (MQ=255)
atgGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCa                                                                                                                             >  7:49407/1‑139 (MQ=255)
     gAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGCATGACGTCGAGCTTCACGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTaa                                                                                                                         >  2:153766/1‑138 (MQ=255)
     gAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTaa                                                                                                                         >  5:240122/1‑138 (MQ=255)
      acgcgcCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGCGCAGCAGCATCCGCCGGTTTTTCCTCCCCGTGAAGATGAATGACGTAGCGCTTCAAGCTCGT‑GTCAATACGTGACTTCGTCTCTATTTATTCAATAAc                                                                                                                        >  4:236920/3‑138 (MQ=255)
      aaGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATAACCGTGAAGCTGAATGACGTCGAGATTCAAGCTCTT‑GGCAATACGTGACATCGACTCTATTGATTCAATAAc                                                                                                                        >  8:6557/1‑138 (MQ=255)
              tCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAAcacc                                                                                                               >  5:118448/1‑139 (MQ=255)
                     ttCACAGCCTGTCAGCTTGCGAAGCGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATGCATTAACAAAAACACCGGCAAcc                                                                                                        >  7:397797/1‑139 (MQ=255)
                          agccTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAAc                                                                                                         <  8:140074/130‑1 (MQ=255)
                          agccTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATAACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAAc                                                                                                         >  7:140074/4‑133 (MQ=255)
                              tGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACTGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTc                                                                                                >  5:335189/1‑138 (MQ=255)
                              tGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTc                                                                                                >  7:391604/1‑138 (MQ=255)
                               gTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGttttt                                                                                                 >  6:123200/1‑136 (MQ=255)
                               gTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGttttt                                                                                                 <  5:123200/136‑1 (MQ=255)
                               gTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGa                                                                                              >  1:229942/1‑139 (MQ=255)
                                tCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAGGCTCTT‑GGCATTACGTGACATCGTCTCGATTGATTCATTAGCAAAAACACCTGCACCCTGTTTTTCGac                                                                                             >  2:287172/1‑139 (MQ=255)
                                tCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGa                                                                                              <  2:413446/138‑1 (MQ=255)
                                tCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTT‑TTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGa                                                                                              <  8:170366/137‑1 (MQ=255)
                                tCAGCTTGCGAACAGCAGCAGCATCCGCCTGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGa                                                                                              <  6:13699/138‑1 (MQ=255)
                                  aGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGATGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGacac                                                                                           <  1:157108/139‑1 (MQ=255)
                                  aGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGacac                                                                                           <  8:391604/139‑1 (MQ=255)
                                                       tCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATtttt                                                                      >  4:370674/1‑139 (MQ=255)
                                                       tCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATtttt                                                                      >  1:226304/1‑139 (MQ=255)
                                                       tCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATtttt                                                                      >  1:250719/1‑139 (MQ=255)
                                                         cGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTc                                                                    <  6:118448/139‑1 (MQ=255)
                                                              gtttttcatcaCTGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATc                                                               <  6:335189/134‑1 (MQ=255)
                                                                    catcaCCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCg                                                          <  8:49407/138‑1 (MQ=255)
                                                                      tcaCCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGgag                                                       <  4:296936/139‑1 (MQ=255)
                                                                            tGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACtt                                                 >  1:180546/1‑139 (MQ=255)
                                                                                    aaTGACGTCGAGCTTCAAGCTCTT‑GGCATTACGTGACATCGTCTCTATTGATTCATTAACAAAAACCCCTGCACCCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCgtttg                                         >  7:13165/1‑135 (MQ=255)
                                                                                    aaTGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATGATCCGGAGATACTTTTCgtttg                                         >  4:185505/1‑135 (MQ=255)
                                                                                      tGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCt                                       >  1:129384/1‑139 (MQ=255)
                                                                                                    aaG‑TCTTCGGCAATCCGTGACATCGTCTCTATCGATTCATTAACAAAAACCCCTGCAACCTGTTTTTCGACACAAACTTGACTCAGCCACTTTTTCACATCTTGCGGAGATACTTTTCGGTAGCTTTCAGCAAAAATa                          >  4:100151/1‑138 (MQ=255)
                                                                                                           ttggCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATcc                     >  7:106418/3‑137 (MQ=255)
                                                                                                                 atacGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAAt             <  3:370674/136‑1 (MQ=255)
                                                                                                                 atacGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAAt             <  2:250719/136‑1 (MQ=255)
                                                                                                                       gACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGCCACGAACTTGGTTCAGCCATTTTTTCACATCTTCAGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCt       >  5:236134/1‑139 (MQ=255)
                                                                                                                       gACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCt       >  5:132314/1‑139 (MQ=255)
                                                                                                                        aCATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCtg      >  3:49789/1‑139 (MQ=255)
                                                                                                                            cGTCTCTATTGATTCATTAACAAAAACACCTGCTACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTGCAGGAGATAATTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCATGTGat  >  5:90052/1‑138 (MQ=255)
                                                                                                                            cGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGat  >  5:169555/1‑138 (MQ=255)
                                                                                                                                     |                                                                                                                                 
ATGGTGAAGCGCCTTCCATATTTCACAATCTGTCAGCATGCGAAGCGCATCGGCATCCGCAGGTTCCTCATCACCGTGAAGCTGAATGACGTCTAGATTTAAG‑TCTTCGGCAATCCGTGACATCGTCTCTATCGATTCATTAACAAAAACCCCTGCAACCTGTTTTTCGACACAAACTTGACTCAGCCACTTTTTCACATCTTGCGGAGATACTTTTCGGTAGCTTTCAGCAAAAATAAAGCCCAGATAATCCGCCTGTGAG  >  CP006881/2191670‑2191931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑