| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 2,191,859 | C→T | K46K (AAG→AAA) | U712_10980 ← | N‑(5'‑phosphoribosyl)anthranilate isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,191,859 | 0 | C | T | 94.7% | 104.0 / ‑2.3 | 38 | K46K (AAG→AAA) | U712_10980 | N‑(5'‑phosphoribosyl)anthranilate isomerase |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (21/15); total (22/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01 | |||||||||||
TCCGCAGGTTCCTCATCACCGTGAAGCTGAATGACGTCTAGATTTAAG‑TCTTCGGCAATCCGTGACATCGTCTCTATCGATTCATTAACAAAAACCCCTGCAACCTGTTTTTCGACACAAACTTGACTCAGCCACTTTTTCACATCTTGCGGAGATACTTTTCGGTAGCTTTCAGCAAAAATAAAGCCCAGATAATCCGCCTGTGAGTCTGCTGCAAGCTGCAAATCCTGCAGTGACCGTATGCCGCAATATTTTAATGCCGGCTTCTT > CP006881/2191725‑2191993 | tCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATtttt > 1:226304/1‑139 (MQ=255)tCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATtttt > 1:250719/1‑139 (MQ=255)tCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATtttt > 4:370674/1‑139 (MQ=255) cGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTc < 6:118448/139‑1 (MQ=255) gtttttcatcaCTGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATc < 6:335189/134‑1 (MQ=255) catcaCCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCg < 8:49407/138‑1 (MQ=255) tcaCCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGgag < 4:296936/139‑1 (MQ=255) tGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACtt > 1:180546/1‑139 (MQ=255) aaTGACGTCGAGCTTCAAGCTCTT‑GGCATTACGTGACATCGTCTCTATTGATTCATTAACAAAAACCCCTGCACCCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCgtttg > 7:13165/1‑135 (MQ=255) aaTGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATGATCCGGAGATACTTTTCgtttg > 4:185505/1‑135 (MQ=255) tGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCt > 1:129384/1‑139 (MQ=255) aaG‑TCTTCGGCAATCCGTGACATCGTCTCTATCGATTCATTAACAAAAACCCCTGCAACCTGTTTTTCGACACAAACTTGACTCAGCCACTTTTTCACATCTTGCGGAGATACTTTTCGGTAGCTTTCAGCAAAAATa > 4:100151/1‑138 (MQ=255) ttggCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATcc > 7:106418/3‑137 (MQ=255) atacGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAAt < 2:250719/136‑1 (MQ=255) atacGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAAt < 3:370674/136‑1 (MQ=255) gACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGCCACGAACTTGGTTCAGCCATTTTTTCACATCTTCAGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCt > 5:236134/1‑139 (MQ=255) gACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCt > 5:132314/1‑139 (MQ=255) aCATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCtg > 3:49789/1‑139 (MQ=255) cGTCTCTATTGATTCATTAACAAAAACACCTGCTACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTGCAGGAGATAATTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCATGTGat > 5:90052/1‑138 (MQ=255) cGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGat > 5:169555/1‑138 (MQ=255) gATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgca < 4:128612/138‑1 (MQ=255) aTTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgcaag > 2:309513/1‑139 (MQ=255) tCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgcaagct > 2:86897/1‑139 (MQ=255) tCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgcaagct > 8:342510/1‑139 (MQ=255) aTTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgcaagctgc < 1:86897/139‑1 (MQ=255) aTTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgcaagctgc < 2:229942/139‑1 (MQ=255) ttAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgcaagctgc > 8:75322/1‑138 (MQ=255) aaCAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgcaagctgcaa < 1:28968/138‑1 (MQ=255) caccTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCtttag < 8:397797/137‑5 (MQ=255) tttttCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGa < 4:361112/129‑1 (MQ=255) tttttCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGa > 3:361112/1‑129 (MQ=255) tttttCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAAtac < 8:71334/137‑3 (MQ=255) tttttCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCg > 4:438543/1‑139 (MQ=255) tCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCaa > 8:190192/1‑138 (MQ=255) ggttCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGcc < 6:240122/134‑1 (MQ=255) aCTCAGCCACTTTTTCACATCTTGCGGAGATACTTTTCGGTAGCTTTCAGCAAAAATAAAGCCCAGATAATCCGCCTGTGAGTCTGCTGCAAGCTGCAAATCCTGCAGTGACCGTATGCCGCAATATTTTAATGCCGGc < 3:100151/139‑1 (MQ=255) gCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCa < 3:439239/118‑1 (MQ=255) ccATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGtttctt > 6:272529/1‑138 (MQ=255) | TCCGCAGGTTCCTCATCACCGTGAAGCTGAATGACGTCTAGATTTAAG‑TCTTCGGCAATCCGTGACATCGTCTCTATCGATTCATTAACAAAAACCCCTGCAACCTGTTTTTCGACACAAACTTGACTCAGCCACTTTTTCACATCTTGCGGAGATACTTTTCGGTAGCTTTCAGCAAAAATAAAGCCCAGATAATCCGCCTGTGAGTCTGCTGCAAGCTGCAAATCCTGCAGTGACCGTATGCCGCAATATTTTAATGCCGGCTTCTT > CP006881/2191725‑2191993 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |