Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,191,891	A→T	Y36K (TAC→AAA)	U712_10980 ←	N‑(5'‑phosphoribosyl)anthranilate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,191,891	0	A	T	95.7%	125.8 / ‑1.8	46	Y36K (TAC→AAA)	U712_10980	N‑(5'‑phosphoribosyl)anthranilate isomerase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base T (21/23);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.18e-01

TGACGTCTAGATTTAAG‑TCTTCGGCAATCCGTGACATCGTCTCTATCGATTCATTAACAAAAACCCCTGCAACCTGTTTTTCGACACAAACTTGACTCAGCCACTTTTTCACATCTTGCGGAGATACTTTTCGGTAGCTTTCAGCAAAAATAAAGCCCAGATAATCCGCCTGTGAGTCTGCTGCAAGCTGCAAATCCTGCAGTGACCGTATGCCGCAATATTTTAATGCCGGCTTCTTCACATCCTCACTCCCCAAACAAAGCATG  >  CP006881/2191756‑2192021                                                                                                                                         |                                                                                                                                   tGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCt                                                                                                                                 >  1:129384/1‑139 (MQ=255)               aaG‑TCTTCGGCAATCCGTGACATCGTCTCTATCGATTCATTAACAAAAACCCCTGCAACCTGTTTTTCGACACAAACTTGACTCAGCCACTTTTTCACATCTTGCGGAGATACTTTTCGGTAGCTTTCAGCAAAAATa                                                                                                                    >  4:100151/1‑138 (MQ=255)                      ttggCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATcc                                                                                                               >  7:106418/3‑137 (MQ=255)                            atacGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAAt                                                                                                       <  3:370674/136‑1 (MQ=255)                            atacGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAAt                                                                                                       <  2:250719/136‑1 (MQ=255)                                  gACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGCCACGAACTTGGTTCAGCCATTTTTTCACATCTTCAGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCt                                                                                                 >  5:236134/1‑139 (MQ=255)                                  gACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCt                                                                                                 >  5:132314/1‑139 (MQ=255)                                   aCATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCtg                                                                                                >  3:49789/1‑139 (MQ=255)                                       cGTCTCTATTGATTCATTAACAAAAACACCTGCTACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTGCAGGAGATAATTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCATGTGat                                                                                            >  5:90052/1‑138 (MQ=255)                                       cGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGat                                                                                            >  5:169555/1‑138 (MQ=255)                                                 gATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgca                                                                                   <  4:128612/138‑1 (MQ=255)                                                  aTTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgcaag                                                                                 >  2:309513/1‑139 (MQ=255)                                                    tCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgcaagct                                                                               >  2:86897/1‑139 (MQ=255)                                                    tCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgcaagct                                                                               >  8:342510/1‑139 (MQ=255)                                                      aTTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgcaagctgc                                                                             <  2:229942/139‑1 (MQ=255)                                                      aTTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgcaagctgc                                                                             <  1:86897/139‑1 (MQ=255)                                                       ttAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgcaagctgc                                                                             >  8:75322/1‑138 (MQ=255)                                                         aaCAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgcaagctgcaa                                                                           <  1:28968/138‑1 (MQ=255)                                                                 caccTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCtttag                                                                  <  8:397797/137‑5 (MQ=255)                                                                              tttttCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGa                                                               <  4:361112/129‑1 (MQ=255)                                                                              tttttCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGa                                                               >  3:361112/1‑129 (MQ=255)                                                                              tttttCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAAtac                                                       <  8:71334/137‑3 (MQ=255)                                                                              tttttCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCg                                                     >  4:438543/1‑139 (MQ=255)                                                                                  tCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCaa                                                  >  8:190192/1‑138 (MQ=255)                                                                                               ggttCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGcc                                      <  6:240122/134‑1 (MQ=255)                                                                                                aCTCAGCCACTTTTTCACATCTTGCGGAGATACTTTTCGGTAGCTTTCAGCAAAAATAAAGCCCAGATAATCCGCCTGTGAGTCTGCTGCAAGCTGCAAATCCTGCAGTGACCGTATGCCGCAATATTTTAATGCCGGc                                   <  3:100151/139‑1 (MQ=255)                                                                                                     gCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCa                                                   <  3:439239/118‑1 (MQ=255)                                                                                                      ccATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGtttctt                              >  6:272529/1‑138 (MQ=255)                                                                                                       cattatttCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTca                            <  1:287172/134‑1 (MQ=255)                                                                                                         ttttttCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTcaca                          <  7:190192/138‑1 (MQ=255)                                                                                                               cacaTCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTc                     <  5:272529/138‑1 (MQ=255)                                                                                                               cacaTCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTc                     <  4:49789/138‑1 (MQ=255)                                                                                                                acaTCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCa                    <  2:226304/138‑1 (MQ=255)                                                                                                                acaTCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCAc                   <  6:132314/139‑1 (MQ=255)                                                                                                                  aTCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTc                 >  6:429256/1‑139 (MQ=255)                                                                                                                       ccGGAGATACTTTTCGTTTGCTTTCAGCAGAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTcc                <  8:212658/134‑1 (MQ=255)                                                                                                                       ccGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTcc                <  8:302110/134‑1 (MQ=255)                                                                                                                       ccGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGACTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTcc                <  8:106418/134‑1 (MQ=255)                                                                                                                         ggAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCATTTAGTGACCGAATACCGCAATATTTTTATGCCGGTTTCTTCACAACCTCACTCCCtaaac          <  7:6557/139‑1 (MQ=255)                                                                                                                            gaTACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCtaaaca         >  7:413867/1‑137 (MQ=255)                                                                                                                              tACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGc     <  8:413867/139‑1 (MQ=255)                                                                                                                               aCTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATTCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCa    >  4:32547/1‑139 (MQ=255)                                                                                                                                 ttttCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATg  >  6:18173/1‑139 (MQ=255)                                                                                                                                 ttttCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATg  >  4:142901/1‑139 (MQ=255)                                                                                                                                  tttCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCa                    <  1:126483/120‑1 (MQ=255)                                                                                                                                  tttCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCa                    >  2:126483/1‑120 (MQ=255)                                                                                                                                         |                                                                                                                                   TGACGTCTAGATTTAAG‑TCTTCGGCAATCCGTGACATCGTCTCTATCGATTCATTAACAAAAACCCCTGCAACCTGTTTTTCGACACAAACTTGACTCAGCCACTTTTTCACATCTTGCGGAGATACTTTTCGGTAGCTTTCAGCAAAAATAAAGCCCAGATAATCCGCCTGTGAGTCTGCTGCAAGCTGCAAATCCTGCAGTGACCGTATGCCGCAATATTTTAATGCCGGCTTCTTCACATCCTCACTCCCCAAACAAAGCATG  >  CP006881/2191756‑2192021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑