Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,191,916 A→G Y27Y (TAT→TAC)  U712_10980 ← N‑(5'‑phosphoribosyl)anthranilate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,191,9160AG97.8% 141.2 / ‑4.7 46Y27Y (TAT→TAC) U712_10980N‑(5'‑phosphoribosyl)anthranilate isomerase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (19/26);  total (19/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.31e-01

ATCCGTGACATCGTCTCTATCGATTCATTAACAAAAACCCCTGCAACCTGTTTTTCGACACAAACTTGACTCAGCCACTTTTTCACATCTTGCGGAGATACTTTTCGGTAGCTTTCAGCAAAAATAAAGCCCAGATAATCCGCCTGTGAGTCTGCTGCAAGCTGCAAATCCTGCAGTGACCGTATGCCGCAATATTTTAATGCCGGCTTCTTCACATCCTCACTCCCCAAACAAAGCATGGATTGCTTTTTG  >  CP006881/2191782‑2192033
                                                                                                                                      |                                                                                                                     
atacGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAAt                                                                                                                   <  2:250719/136‑1 (MQ=255)
atacGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAAt                                                                                                                   <  3:370674/136‑1 (MQ=255)
      gACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGCCACGAACTTGGTTCAGCCATTTTTTCACATCTTCAGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCt                                                                                                             >  5:236134/1‑139 (MQ=255)
      gACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCt                                                                                                             >  5:132314/1‑139 (MQ=255)
       aCATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCtg                                                                                                            >  3:49789/1‑139 (MQ=255)
           cGTCTCTATTGATTCATTAACAAAAACACCTGCTACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTGCAGGAGATAATTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCATGTGat                                                                                                        >  5:90052/1‑138 (MQ=255)
           cGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGat                                                                                                        >  5:169555/1‑138 (MQ=255)
                     gATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgca                                                                                               <  4:128612/138‑1 (MQ=255)
                      aTTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgcaag                                                                                             >  2:309513/1‑139 (MQ=255)
                        tCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgcaagct                                                                                           >  8:342510/1‑139 (MQ=255)
                        tCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgcaagct                                                                                           >  2:86897/1‑139 (MQ=255)
                          aTTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgcaagctgc                                                                                         <  1:86897/139‑1 (MQ=255)
                          aTTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgcaagctgc                                                                                         <  2:229942/139‑1 (MQ=255)
                           ttAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgcaagctgc                                                                                         >  8:75322/1‑138 (MQ=255)
                             aaCAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgcaagctgcaa                                                                                       <  1:28968/138‑1 (MQ=255)
                                     caccTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCtttag                                                                              <  8:397797/137‑5 (MQ=255)
                                                  tttttCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGa                                                                           <  4:361112/129‑1 (MQ=255)
                                                  tttttCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGa                                                                           >  3:361112/1‑129 (MQ=255)
                                                  tttttCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAAtac                                                                   <  8:71334/137‑3 (MQ=255)
                                                  tttttCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCg                                                                 >  4:438543/1‑139 (MQ=255)
                                                      tCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCaa                                                              >  8:190192/1‑138 (MQ=255)
                                                                   ggttCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGcc                                                  <  6:240122/134‑1 (MQ=255)
                                                                    aCTCAGCCACTTTTTCACATCTTGCGGAGATACTTTTCGGTAGCTTTCAGCAAAAATAAAGCCCAGATAATCCGCCTGTGAGTCTGCTGCAAGCTGCAAATCCTGCAGTGACCGTATGCCGCAATATTTTAATGCCGGc                                               <  3:100151/139‑1 (MQ=255)
                                                                         gCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCa                                                               <  3:439239/118‑1 (MQ=255)
                                                                          ccATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGtttctt                                          >  6:272529/1‑138 (MQ=255)
                                                                           cattatttCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTca                                        <  1:287172/134‑1 (MQ=255)
                                                                             ttttttCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTcaca                                      <  7:190192/138‑1 (MQ=255)
                                                                                   cacaTCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTc                                 <  5:272529/138‑1 (MQ=255)
                                                                                   cacaTCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTc                                 <  4:49789/138‑1 (MQ=255)
                                                                                    acaTCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCa                                <  2:226304/138‑1 (MQ=255)
                                                                                    acaTCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCAc                               <  6:132314/139‑1 (MQ=255)
                                                                                      aTCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTc                             >  6:429256/1‑139 (MQ=255)
                                                                                           ccGGAGATACTTTTCGTTTGCTTTCAGCAGAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTcc                            <  8:212658/134‑1 (MQ=255)
                                                                                           ccGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTcc                            <  8:302110/134‑1 (MQ=255)
                                                                                           ccGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGACTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTcc                            <  8:106418/134‑1 (MQ=255)
                                                                                             ggAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCATTTAGTGACCGAATACCGCAATATTTTTATGCCGGTTTCTTCACAACCTCACTCCCtaaac                      <  7:6557/139‑1 (MQ=255)
                                                                                                gaTACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCtaaaca                     >  7:413867/1‑137 (MQ=255)
                                                                                                  tACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGc                 <  8:413867/139‑1 (MQ=255)
                                                                                                   aCTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATTCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCa                >  4:32547/1‑139 (MQ=255)
                                                                                                     ttttCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATg              >  6:18173/1‑139 (MQ=255)
                                                                                                     ttttCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATg              >  4:142901/1‑139 (MQ=255)
                                                                                                      tttCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCa                                <  1:126483/120‑1 (MQ=255)
                                                                                                      tttCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCa                                >  2:126483/1‑120 (MQ=255)
                                                                                                             tgCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCt       <  6:236134/137‑1 (MQ=255)
                                                                                                               cTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCttt     <  1:7885/138‑1 (MQ=255)
                                                                                                                  tCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCtttacg  <  1:439194/138‑4 (MQ=255)
                                                                                                                                      |                                                                                                                     
ATCCGTGACATCGTCTCTATCGATTCATTAACAAAAACCCCTGCAACCTGTTTTTCGACACAAACTTGACTCAGCCACTTTTTCACATCTTGCGGAGATACTTTTCGGTAGCTTTCAGCAAAAATAAAGCCCAGATAATCCGCCTGTGAGTCTGCTGCAAGCTGCAAATCCTGCAGTGACCGTATGCCGCAATATTTTAATGCCGGCTTCTTCACATCCTCACTCCCCAAACAAAGCATGGATTGCTTTTTG  >  CP006881/2191782‑2192033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑