Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,191,931 G→T D22E (GAC→GAA)  U712_10980 ← N‑(5'‑phosphoribosyl)anthranilate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,191,9310GT98.2% 160.9 / ‑3.8 56D22E (GAC→GAA) U712_10980N‑(5'‑phosphoribosyl)anthranilate isomerase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (20/35);  total (20/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.01e-01

GATTCATTAACAAAAACCCCTGCAACCTGTTTTTCGACACAAACTTGACTCAGCCACTTTTTCACATCTTGCGGAGATACTTTTCGGTAGCTTTCAGCAAAAATAAAGCCCAGATAATCCGCCTGTGAGTCTGCTGCAAGCTGCAAATCCTGCAGTGACCGTATGCCGCAATATTTTAATGCCGGCTTCTTCACATCCTCACTCCCCAAACAAAGCATGGATTGCTTTTTGCTGTGATGTTTGTCTCATCAATGATTCACCAAT  >  CP006881/2191803‑2192066
                                                                                                                                |                                                                                                                                       
gATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgca                                                                                                                                <  4:128612/138‑1 (MQ=255)
 aTTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgcaag                                                                                                                              >  2:309513/1‑139 (MQ=255)
   tCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgcaagct                                                                                                                            >  8:342510/1‑139 (MQ=255)
   tCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgcaagct                                                                                                                            >  2:86897/1‑139 (MQ=255)
     aTTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgcaagctgc                                                                                                                          <  1:86897/139‑1 (MQ=255)
     aTTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgcaagctgc                                                                                                                          <  2:229942/139‑1 (MQ=255)
      ttAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgcaagctgc                                                                                                                          >  8:75322/1‑138 (MQ=255)
        aaCAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCgccgcaagctgcaa                                                                                                                        <  1:28968/138‑1 (MQ=255)
                caccTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCtttag                                                                                                               <  8:397797/137‑5 (MQ=255)
                             tttttCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGa                                                                                                            <  4:361112/129‑1 (MQ=255)
                             tttttCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGa                                                                                                            >  3:361112/1‑129 (MQ=255)
                             tttttCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAAtac                                                                                                    <  8:71334/137‑3 (MQ=255)
                             tttttCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCg                                                                                                  >  4:438543/1‑139 (MQ=255)
                                 tCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCaa                                                                                               >  8:190192/1‑138 (MQ=255)
                                              ggttCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGcc                                                                                   <  6:240122/134‑1 (MQ=255)
                                               aCTCAGCCACTTTTTCACATCTTGCGGAGATACTTTTCGGTAGCTTTCAGCAAAAATAAAGCCCAGATAATCCGCCTGTGAGTCTGCTGCAAGCTGCAAATCCTGCAGTGACCGTATGCCGCAATATTTTAATGCCGGc                                                                                <  3:100151/139‑1 (MQ=255)
                                                    gCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCa                                                                                                <  3:439239/118‑1 (MQ=255)
                                                     ccATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGtttctt                                                                           >  6:272529/1‑138 (MQ=255)
                                                      cattatttCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTca                                                                         <  1:287172/134‑1 (MQ=255)
                                                        ttttttCACATCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTcaca                                                                       <  7:190192/138‑1 (MQ=255)
                                                              cacaTCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTc                                                                  <  5:272529/138‑1 (MQ=255)
                                                              cacaTCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTc                                                                  <  4:49789/138‑1 (MQ=255)
                                                               acaTCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCa                                                                 <  2:226304/138‑1 (MQ=255)
                                                               acaTCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCAc                                                                <  6:132314/139‑1 (MQ=255)
                                                                 aTCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTc                                                              >  6:429256/1‑139 (MQ=255)
                                                                      ccGGAGATACTTTTCGTTTGCTTTCAGCAGAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTcc                                                             <  8:212658/134‑1 (MQ=255)
                                                                      ccGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTcc                                                             <  8:302110/134‑1 (MQ=255)
                                                                      ccGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGACTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTcc                                                             <  8:106418/134‑1 (MQ=255)
                                                                        ggAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCATTTAGTGACCGAATACCGCAATATTTTTATGCCGGTTTCTTCACAACCTCACTCCCtaaac                                                       <  7:6557/139‑1 (MQ=255)
                                                                           gaTACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCtaaaca                                                      >  7:413867/1‑137 (MQ=255)
                                                                             tACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGc                                                  <  8:413867/139‑1 (MQ=255)
                                                                              aCTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATTCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCa                                                 >  4:32547/1‑139 (MQ=255)
                                                                                ttttCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATg                                               >  4:142901/1‑139 (MQ=255)
                                                                                ttttCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATg                                               >  6:18173/1‑139 (MQ=255)
                                                                                 tttCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCa                                                                 >  2:126483/1‑120 (MQ=255)
                                                                                 tttCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCa                                                                 <  1:126483/120‑1 (MQ=255)
                                                                                        tgCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCt                                        <  6:236134/137‑1 (MQ=255)
                                                                                          cTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCttt                                      <  1:7885/138‑1 (MQ=255)
                                                                                             tCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCtttacg                                   <  1:439194/138‑4 (MQ=255)
                                                                                                                     tcagCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGAt          >  8:421682/4‑139 (MQ=255)
                                                                                                                     tcagCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGAt          >  7:231765/4‑139 (MQ=255)
                                                                                                                     tcagCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGAATTGATTGCTTTCCGCTGAGAAGTTTGTCTCATCAATGa           >  2:210275/4‑138 (MQ=255)
                                                                                                                        gcatgtgATTCAGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCTATGATTCa       <  3:136078/136‑1 (MQ=255)
                                                                                                                        gCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCa       <  7:75322/139‑1 (MQ=255)
                                                                                                                        gCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCa       <  8:231765/139‑1 (MQ=255)
                                                                                                                        gCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCa       <  2:171726/139‑1 (MQ=255)
                                                                                                                         ccTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCAc                                                                >  3:274852/1‑81 (MQ=255)
                                                                                                                         ccTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCAc                                                                <  4:274852/81‑1 (MQ=255)
                                                                                                                           tgtgATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCAcc     >  5:129009/1‑138 (MQ=255)
                                                                                                                            gtgATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTcc                                                             <  3:351695/81‑1 (MQ=255)
                                                                                                                            gtgATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTcc                                                             >  4:351695/1‑81 (MQ=255)
                                                                                                                             tgATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGAt  <  5:18173/139‑1 (MQ=255)
                                                                                                                             tgATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGAt  <  3:32547/139‑1 (MQ=255)
                                                                                                                             tgATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGAt  <  3:142901/139‑1 (MQ=255)
                                                                                                                             tgATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGAt  <  2:180546/139‑1 (MQ=255)
                                                                                                                             tgATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGAt  <  3:236920/139‑1 (MQ=255)
                                                                                                                                |                                                                                                                                       
GATTCATTAACAAAAACCCCTGCAACCTGTTTTTCGACACAAACTTGACTCAGCCACTTTTTCACATCTTGCGGAGATACTTTTCGGTAGCTTTCAGCAAAAATAAAGCCCAGATAATCCGCCTGTGAGTCTGCTGCAAGCTGCAAATCCTGCAGTGACCGTATGCCGCAATATTTTAATGCCGGCTTCTTCACATCCTCACTCCCCAAACAAAGCATGGATTGCTTTTTGCTGTGATGTTTGTCTCATCAATGATTCACCAAT  >  CP006881/2191803‑2192066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑