Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,191,998	T→A	intergenic (‑2/+3)	U712_10980 ← / ← U712_10985	N‑(5'‑phosphoribosyl)anthranilate isomerase/Indole‑3‑glycerol phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,191,998	0	T	A	100.0%	219.0 / NA	72	intergenic (‑2/+3)	U712_10980/U712_10985	N‑(5'‑phosphoribosyl)anthranilate isomerase/Indole‑3‑glycerol phosphate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (33/39);  total (33/39)

CACATCTTGCGGAGATACTTTTCGGTAGCTTTCAGCAAAAATAAAGCCCAGATAATCCGCCTGTGAGTCTGCTGCAAGCTGCAAATCCTGCAGTGACCGTATGCCGCAATATTTTAATGCCGGCTTCTTCACATCCTCACTCCCCAAACAAAGCATGGATTGCTTTTTGCTGTGATGTTTGTCTCATCAATGATTCACCAATAAGCACAGCTCGTGCTCCATGTTCTTTGACAAATGTTAAATGTTCTAAAGAACCGAT  >  CP006881/2191865‑2192123                                                                                                                                      |                                                                                                                              cacaTCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTc                                                                                                                           <  5:272529/138‑1 (MQ=255) cacaTCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTc                                                                                                                           <  4:49789/138‑1 (MQ=255)  acaTCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCa                                                                                                                          <  2:226304/138‑1 (MQ=255)  acaTCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCAc                                                                                                                         <  6:132314/139‑1 (MQ=255)    aTCTTCCGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTc                                                                                                                       >  6:429256/1‑139 (MQ=255)         ccGGAGATACTTTTCGTTTGCTTTCAGCAGAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTcc                                                                                                                      <  8:212658/134‑1 (MQ=255)         ccGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTcc                                                                                                                      <  8:302110/134‑1 (MQ=255)         ccGGAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGACTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTcc                                                                                                                      <  8:106418/134‑1 (MQ=255)           ggAGATACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCATTTAGTGACCGAATACCGCAATATTTTTATGCCGGTTTCTTCACAACCTCACTCCCtaaac                                                                                                                <  7:6557/139‑1 (MQ=255)              gaTACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCtaaaca                                                                                                               >  7:413867/1‑137 (MQ=255)                tACTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGc                                                                                                           <  8:413867/139‑1 (MQ=255)                 aCTTTTCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATTCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCa                                                                                                          >  4:32547/1‑139 (MQ=255)                   ttttCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATg                                                                                                        >  6:18173/1‑139 (MQ=255)                   ttttCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATg                                                                                                        >  4:142901/1‑139 (MQ=255)                    tttCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCa                                                                                                                          <  1:126483/120‑1 (MQ=255)                    tttCGTTTGCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCa                                                                                                                          >  2:126483/1‑120 (MQ=255)                           tgCTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCt                                                                                                 <  6:236134/137‑1 (MQ=255)                             cTTTCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCttt                                                                                               <  1:7885/138‑1 (MQ=255)                                tCAGCAAAAATAAATCCTAGGTAATCAGCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCtttacg                                                                                            <  1:439194/138‑4 (MQ=255)                                                        tcagCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGAt                                                                   >  7:231765/4‑139 (MQ=255)                                                        tcagCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGAt                                                                   >  8:421682/4‑139 (MQ=255)                                                        tcagCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGAATTGATTGCTTTCCGCTGAGAAGTTTGTCTCATCAATGa                                                                    >  2:210275/4‑138 (MQ=255)                                                           gcatgtgATTCAGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCTATGATTCa                                                                <  3:136078/136‑1 (MQ=255)                                                           gCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCa                                                                <  7:75322/139‑1 (MQ=255)                                                           gCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCa                                                                <  2:171726/139‑1 (MQ=255)                                                           gCCTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCa                                                                <  8:231765/139‑1 (MQ=255)                                                            ccTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCAc                                                                                                                         >  3:274852/1‑81 (MQ=255)                                                            ccTGTGATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCAc                                                                                                                         <  4:274852/81‑1 (MQ=255)                                                              tgtgATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCAcc                                                              >  5:129009/1‑138 (MQ=255)                                                               gtgATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTcc                                                                                                                      <  3:351695/81‑1 (MQ=255)                                                               gtgATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTcc                                                                                                                      >  4:351695/1‑81 (MQ=255)                                                                tgATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGAt                                                           <  3:236920/139‑1 (MQ=255)                                                                tgATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGAt                                                           <  2:180546/139‑1 (MQ=255)                                                                tgATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGAt                                                           <  5:18173/139‑1 (MQ=255)                                                                tgATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGAt                                                           <  3:142901/139‑1 (MQ=255)                                                                tgATTCCGCCGCAAGCTGCAAATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGAt                                                           <  3:32547/139‑1 (MQ=255)                                                                      cgccgcaagctgcaaATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGAt                                                           >  5:220417/5‑133 (MQ=255)                                                                      cgccgcaagctgcaaATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGAt                                                           <  6:220417/129‑1 (MQ=255)                                                                      cgccgcaagctgcaaATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGATAAgta                                                      >  5:320007/5‑136 (MQ=255)                                                                      cgccgcaagctgcaaATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGATAAgta                                                      >  3:313926/5‑136 (MQ=255)                                                                      cgccgcaagctgcaaATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGAGAAGtac                                                     >  2:53739/5‑139 (MQ=255)                                                                      cgccgcaagctgcaaATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTATGTCTCATCAATGATTCACCGATAAGtac                                                     >  8:213944/5‑139 (MQ=255)                                                                      cgccgcaagctgcaaATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCATAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGATAAgta                                                      >  7:366902/5‑136 (MQ=255)                                                                          gcaagcttcaaATCCTTTAGTGTCCGAATACCGAAATATTTTAATGCCGGTTTCTTCACAACGTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTTAGAAGTTTTTCTCATCAATTATTCACCGATAAGTACAGCt                                                 <  7:174479/139‑1 (MQ=255)                                                                            aagctgcaaATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGATAAGTACAGCTCg                                               <  2:129384/139‑1 (MQ=255)                                                                             cgctgcaaATCCTTTAGTGACCGAATACCGCAATATTGTAATGCCGGGTTCTTCACAACCTCCCTCCCTAAACAAAGCATGGATTGCGTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGATAAGTACAGCTCgc                                              <  6:90052/138‑2 (MQ=255)                                                                             agctgcaaATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGATAAGTACAGCTCgc                                              <  6:169555/139‑2 (MQ=255)                                                                                tgcaaATCCTTTAGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGATAAGTACAGCTCgcgcc                                           >  7:322020/1‑138 (MQ=255)                                                                                        ctttaGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGATAAGTACAGCTCGCGCCCCATGtt                                    >  3:123493/5‑138 (MQ=255)                                                                                          ttaGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCtaa                                                                                                                  >  7:207779/3‑58 (MQ=25)                                                                                          ttaGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCtaa                                                                                                                  <  8:207779/56‑1 (MQ=255)                                                                                          ttaGTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGATAAGTACAGCTCGCGCCCCATGTTcat                                 >  2:27718/3‑137 (MQ=255)                                                                                             gTGACCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGATAAGTACAGCTCGCGCCCCATGTTCATTGa                              <  6:320007/139‑1 (MQ=255)                                                                                                aCCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGATAAGTACAGCTCGCGCCCCATGTTCATTGACa                            >  6:249820/1‑138 (MQ=255)                                                                                                aCCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGATAAGTACAGCTCGCGCCCCATGTTCATTGACa                            >  5:44301/1‑138 (MQ=255)                                                                                                aCCGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGATAAGTACAGCTCGCGCCCCATGTTCATTGACa                            <  8:322020/138‑1 (MQ=255)                                                                                                 ccGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGa                                                                                                      <  6:182234/63‑1 (MQ=255)                                                                                                 ccGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGa                                                                                                      >  5:182234/1‑63 (MQ=255)                                                                                                 ccGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCa                                                                >  8:234586/1‑101 (MQ=255)                                                                                                 ccGAATACCGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCa                                                                <  7:234586/101‑1 (MQ=255)                                                                                                        ccGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGATAAGTACAGCTCGCGCCCCATGTTCATTGACaaatgttaa                    <  1:69757/138‑1 (MQ=255)                                                                                                        ccGCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGATAAGTACAGCTCGCGCCCCATGTTCATTGACaaatgttaa                    <  3:185505/138‑1 (MQ=255)                                                                                                          gCAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGATAAGTACAGCTCGCGCCCCATGTTCATTGACaaatgttaaatg                 >  8:423483/1‑139 (MQ=255)                                                                                                           cAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGATAAGTACAGCTCGCGCCCCa                                        <  5:367069/115‑1 (MQ=255)                                                                                                           cAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGATAAGTACAGCTCGCGCCCCa                                        >  6:367069/1‑115 (MQ=255)                                                                                                           cAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGATAAGTACAGCTCGCGCCCCATGTTCATTGACaaatgttaaatgt                >  6:430775/1‑139 (MQ=255)                                                                                                           cAATATTTTAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGAATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATGCACCGATAAGTACAGCTCGCTCCCCATGTTCATTGACaaatgtgaaatgt                >  4:172714/1‑139 (MQ=255)                                                                                                                  ttAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGATAAGTACAGCTCGCGCCCCATGTTCATTGACAAATGTTAAATGTTCTa            >  3:379803/1‑136 (MQ=255)                                                                                                                   tAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGATAAGTACAGCTCg                                               <  8:60034/100‑1 (MQ=255)                                                                                                                   tAATGCCGGTTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGATAAGTACAGCTCg                                               >  7:60034/1‑100 (MQ=255)                                                                                                                   tAATGCCGGCTTCTTCACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTCACCGATAAGTACAGCTCGCGCCCCATGTTCATTGACAAATGTTAAATGTTCTAAAg         <  1:153766/138‑1 (MQ=255)                                                                                                                          ggtttcttcACAACCTCACTCCCTAAACAAAGCATGGATTGCTTTACGCTGAGAAGTTTGTCTCATCAATGATTAACCGATAAGAACAGCTCGCGCCCCATGTTCATTGACAAATGTTAAATGTTATAAAGAACCGAt  >  3:426406/4‑138 (MQ=255)                                                                                                                                      |                                                                                                                              CACATCTTGCGGAGATACTTTTCGGTAGCTTTCAGCAAAAATAAAGCCCAGATAATCCGCCTGTGAGTCTGCTGCAAGCTGCAAATCCTGCAGTGACCGTATGCCGCAATATTTTAATGCCGGCTTCTTCACATCCTCACTCCCCAAACAAAGCATGGATTGCTTTTTGCTGTGATGTTTGTCTCATCAATGATTCACCAATAAGCACAGCTCGTGCTCCATGTTCTTTGACAAATGTTAAATGTTCTAAAGAACCGAT  >  CP006881/2191865‑2192123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑