Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,173,535 A→G S71S (AGT→AGC)  U712_10870 ← Bifunctional protein BirA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,173,5350AG98.0% 150.3 / ‑4.7 50S71S (AGT→AGC) U712_10870Bifunctional protein BirA
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (17/32);  total (18/32)
Fisher's exact test for biased strand distribution p-value = 3.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.98e-01

CCACCACAAGAGTGCCTTCCGGTGCGTTATTATTCGCAAGCTCATGCGCCGTTTTTTGCGTGCTTGAAATAACGTCTTGATAAATAAGATGCTGGCCCATTACTTCCGTTTTCAATCCGAAGCGAATTTCACTTTCACTGAGTTTCCCGGGCTTTTTGATAAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAGCCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTGGAACAGCCGA  >  CP006881/2173405‑2173659
                                                                                                                                  |                                                                                                                            
ccaccaCAAGGGTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATttcgctttcact                                                                                                                      <  6:182885/139‑1 (MQ=255)
          ggTGCCTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTGTTTTGGGTGCTTGAAAGAACGTCATGGTAAATAAGATTCTGGCCCATCACTTCCGTTTTTAATACAAAAAGAATTTCGCTTTCACTGAGttttgcg                                                                                                            >  3:268365/2‑135 (MQ=255)
              ccTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCgggtt                                                                                                        >  6:71674/1‑137 (MQ=255)
              ccTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTGTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGGTTGCCCATCACTTCCGTTTTTTATGGAAAAAGAATTTTGAGTTCAATGAGTTTTCCggggg                                                                                                        >  4:43951/1‑137 (MQ=255)
              ccTTCCGGTGCGTTATTATTCGCGAGCTCATGAGCCGTGTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGGTGGCCCCTCACTTCCGTTTTTTATCCAAACAGAATTTCGCATTCACTGAGTTTTACgggtt                                                                                                        >  8:111011/1‑137 (MQ=255)
                   cGGTGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTg                                                                                                   <  5:284325/139‑1 (MQ=255)
                     gTGCGTTATTATTCGCGAGCTCATGATCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGATTTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGAt                                                                                                 <  3:26390/139‑1 (MQ=255)
                      tGCGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGAtg                                                                                                <  8:2745/139‑2 (MQ=255)
                        cGTTATTATTCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAg                                                                                              <  6:16658/139‑1 (MQ=255)
                               attCGCGAGCTCATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGata                                                                                        <  5:385419/138‑1 (MQ=255)
                                          cATGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATGAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTttcttc                                                                             >  3:128616/1‑138 (MQ=255)
                                           aTGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTa                                                                           <  1:158952/139‑1 (MQ=255)
                                           aTGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTa                                                                           <  3:146728/139‑1 (MQ=255)
                                           aTGAGCCGTTTTTTGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTa                                                                           <  5:71674/139‑1 (MQ=255)
                                           aTGAGCCGTTTTTTGCGCGCTTGAAAGAACGTCATGGTAAATAAGATGCGGGCCCCTCACTTCCGTCTTTAATCCAAAACGAATTTCGCTTTCACTGAGGTTTCCGGGTGTTTTGATGAGCCGATATCCTTTTCTTCTa                                                                           <  8:44210/139‑1 (MQ=255)
                                                   ttttttGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTGGGTTTCAATGAGTTTTACGGGTTTTTTTATGAGCCGATATCCTTTTATTATAACGGCTTc                                                                   >  6:143087/1‑139 (MQ=255)
                                                   ttttttGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTc                                                                   <  4:93439/139‑1 (MQ=255)
                                                      tttGCGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTa                                                                 <  1:246915/138‑1 (MQ=255)
                                                          cGTGCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCa                                                            >  7:68288/1‑139 (MQ=255)
                                                             gCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTa                                                                           >  1:85996/1‑121 (MQ=255)
                                                             gCTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTa                                                                           <  2:85996/121‑1 (MQ=255)
                                                              cTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTc                                                                   <  6:75035/128‑1 (MQ=255)
                                                              cTTGAAAGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTc                                                                   >  5:75035/1‑128 (MQ=255)
                                                                  aaaGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCct                                                     <  7:77809/138‑1 (MQ=255)
                                                                  aaaGAACGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCct                                                     <  2:106015/138‑1 (MQ=255)
                                                                        cGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCg                                              >  3:79947/1‑139 (MQ=255)
                                                                        cGTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCg                                              <  3:170857/139‑1 (MQ=255)
                                                                         gTCATGGTAAATAAGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGa                                             <  2:58054/139‑1 (MQ=255)
                                                                           catggtaaataaGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAg                                           <  3:24353/134‑1 (MQ=255)
                                                                            atggtaaataaGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAg                                           <  4:268365/134‑1 (MQ=255)
                                                                            atggtaaat‑agATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAGct                                         <  1:15746/135‑1 (MQ=255)
                                                                                      aGATGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAata                                 <  7:111011/138‑1 (MQ=255)
                                                                                        aTGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAg                                           <  3:265252/126‑1 (MQ=255)
                                                                                        aTGCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGGTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAg                                           >  4:265252/1‑126 (MQ=255)
                                                                                          gCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCg                                              <  2:144483/121‑1 (MQ=255)
                                                                                          gCTGGCCCATCACTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCg                                              >  1:144483/1‑121 (MQ=255)
                                                                                              gCCCATTACTTCCGTTTTCAATCCGAAGCGAATTTCACTTTCACTGAGTTTCCCGGGCTTTTTGATAAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAGCCCTCTTTCCGAAGCTCTTCAATATGCTTCcac                        >  4:382274/1‑139 (MQ=255)
                                                                                              gCCCATCACTTCGGTTTTTTATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCGTTTTCTTCTAACGGCTTCGACTTCATAACCCTCTTTGCGAAGCACTGCAATATTCTTCca                         >  8:334692/1‑138 (MQ=255)
                                                                                                   tcaCTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTaa                                                                          >  8:265089/3‑84 (MQ=255)
                                                                                                   tcaCTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTaa                                                                          <  7:265089/82‑1 (MQ=255)
                                                                                                   tcaCTTCCGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACAcagc                   <  3:95771/137‑1 (MQ=255)
                                                                                                          cGTTTTTAATCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCtt            >  3:91896/1‑138 (MQ=255)
                                                                                                              tttaaTCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAg                                           >  6:390762/4‑104 (MQ=255)
                                                                                                              tttaaTCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAg                                           <  5:390762/101‑1 (MQ=255)
                                                                                                              tataaTCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGaa         <  8:200186/135‑1 (MQ=255)
                                                                                                                 aaTCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACAcag                    >  6:3461/1‑124 (MQ=255)
                                                                                                                 aaTCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACAcag                    <  5:3461/124‑1 (MQ=255)
                                                                                                                  aTCCAAAACGAATTTCGCTTTCACTGAGTTTTCCTGGTTTGTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTAATTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTCAACAGcc    <  7:334692/139‑1 (MQ=255)
                                                                                                                  aTCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGcc    <  4:100291/139‑1 (MQ=255)
                                                                                                                  aTCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGcc    <  5:143087/139‑1 (MQ=255)
                                                                                                                    ccaaaacGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAGCTGTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGa  <  3:43951/133‑1 (MQ=255)
                                                                                                                                  |                                                                                                                            
CCACCACAAGAGTGCCTTCCGGTGCGTTATTATTCGCAAGCTCATGCGCCGTTTTTTGCGTGCTTGAAATAACGTCTTGATAAATAAGATGCTGGCCCATTACTTCCGTTTTCAATCCGAAGCGAATTTCACTTTCACTGAGTTTCCCGGGCTTTTTGATAAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAGCCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTGGAACAGCCGA  >  CP006881/2173405‑2173659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑