Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,192,273	T→A	T161S (ACA→TCA)	U712_10985 ←	Indole‑3‑glycerol phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,192,273	0	T	A	100.0%	240.5 / NA	76	T161S (ACA→TCA)	U712_10985	Indole‑3‑glycerol phosphate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (25/51);  total (25/51)

CAGTGATTCTTTCGGAGCAAGAGATGCGATTTGTTCTGTCTGCATGATAGAGGTTTCAAAAGTTTTTAGGTTTCGGTTGTTTATGCCGAGAATATCAGGCGTGAATACTTTCAATATTTGTTCTAGCGTTGTTGCATCATGAACCTCCACTAACACGTCCATCTCCTTTTCACCTGCTTCAAGATATAATTCATGAAGGTGTAAGGGATCAAGCACTTCGCCGATTAACAATATGGCATCCGCTCCGATTCTTCTTGATTCCT  >  CP006881/2192142‑2192404                                                                                                                                    |                                                                                                                                    caaggATTCTTTCGGAACGAGAGATGCGATTTGTTCTGTCTGCTTTACAGATGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGcatca                                                                                                                              <  5:299758/135‑1 (MQ=255)   aggATTCTTTCGGAACGAGAGATGCGATTTGTTCTGTCTGCTTTACAGATGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATg                                                                                                                            <  5:430775/137‑1 (MQ=255)    ggATTCTTTCGGAACGAGAGATGCGATTTGTTCTGTCTGCTTTACAGATGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATg                                                                                                                            >  4:381794/2‑138 (MQ=255)      aTTCTTTCGGAACGAGAGATGCGATTTGTTCTGTCTGCTTTACAGATGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGTATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAAc                                                                                                                         <  2:92462/139‑1 (MQ=255)      aTTCTTTCGGAACGAGAGATGCGATTTGTTCTGTCTGCTTTACAGATGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAAc                                                                                                                         <  3:178426/139‑1 (MQ=255)      aTTCTTTCGGAACGAGAGATGCGATTTGTTCTGTCTGCTTTACAGATGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAAc                                                                                                                         <  6:63565/139‑1 (MQ=255)      aTTCTTTCGGAACGAGAGATGCGATTTGTTCTGTCTGCTTTACAGATGTTTCAAACGTTTTTAGGGTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAAc                                                                                                                         <  5:272678/139‑1 (MQ=255)            tCGGAACGAGAGATGCGATTTGTTCTGTCTGCTTTACAGATGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTc                                                                                                                      <  3:121845/136‑1 (MQ=255)            tCGGAACGAGAGATGCGATTTGTTCTGTCTGCTTTACAGATGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCAc                                                                                                                   >  8:370675/1‑139 (MQ=255)               gaacgagagATGCGATTTGTTCTGTCTGCTTTACAGATGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTaa                                                                                                                <  8:273582/134‑1 (MQ=255)               gaacgagagATGCGATTTGTTCTGTCTGCTTTACAGATGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTaa                                                                                                                <  7:92097/134‑1 (MQ=255)               gaacgagagATGCGATTTGTTCTGTCTGCTTTACAGATGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTaa                                                                                                                >  5:138799/6‑139 (MQ=255)                 acgagagATGCGATTTGTTCTGTCTGCTTTACAGATGTTTCAAACTTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAaca                                                                                                              <  3:462/136‑1 (MQ=255)                 acgagagATGCGATTTGTTCTGTCTGCTTTACAGATGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAaca                                                                                                              <  7:282260/136‑1 (MQ=255)                 acgagagATGCGATTTGTTCTGTCTGCTTTACAGATGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAaca                                                                                                              <  1:101437/136‑1 (MQ=255)                 acgagagATGCGATTTGTTCTGTCTGCTTTACAGATGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAaca                                                                                                              <  4:146451/136‑1 (MQ=255)                 acgagagATGCGATTTGTTCTGTCTGCTTTACAGATGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAaca                                                                                                              <  8:409312/136‑1 (MQ=255)                 acgagagATGCGATTTGTTCTGTCTGCTTTACAGATGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGGCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTTATGCATCATGAACCTCCACTAaca                                                                                                              <  1:140179/136‑1 (MQ=255)                         tGCGATTTGTTCTGTCTGCTTTACAGATGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCAtc                                                                                                      <  6:373244/139‑1 (MQ=255)                         tGCGATTTGTTCTGTCTGCTTTACAGATGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCAtc                                                                                                      <  3:381794/139‑1 (MQ=255)                         tGCGATTTGTTCTGTCTGCTTTACAGATGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCAtc                                                                                                      <  7:370675/139‑1 (MQ=255)                                    ctgtctgCTTTACAGATGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAAc                                                                                                                         <  2:251891/109‑1 (MQ=255)                                    ctgtctgCTTTACAGATGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAAc                                                                                                                         >  1:251891/1‑109 (MQ=255)                                       tctgCTTTACAGATGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACt                                                                                                                  >  3:405878/1‑113 (MQ=255)                                       tctgCTTTACAGATGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACt                                                                                                                  <  4:405878/113‑1 (MQ=255)                                       tctgCTTTACAGATGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCAcc                                                                                           <  8:219533/136‑1 (MQ=255)                                       tctgCTTTACAGATGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTg                                                                                         <  3:339298/138‑1 (MQ=255)                                       tctgCTTTACAGATGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGc                                                                                        >  6:448595/1‑139 (MQ=255)                                           ctttacagatGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCtt                                                                                      <  6:332103/131‑1 (MQ=255)                                           ctttacagatGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTc                                                                                     <  5:249820/132‑1 (MQ=255)                                           ctttacagatGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCa                                                                                    <  6:323134/133‑1 (MQ=255)                                           ctttacagatGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCa                                                                                    <  1:313499/133‑1 (MQ=255)                                           ctttacagatGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCa                                                                                    <  5:448595/133‑1 (MQ=255)                                            tttacagatGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTc                                                                                     >  4:424865/6‑137 (MQ=255)                                                cagatGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCAAGata                                                                               <  6:138799/138‑1 (MQ=255)                                                 agatGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCtt                                                                                      >  1:295871/1‑131 (MQ=255)                                                 agatGTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCtt                                                                                      <  2:295871/131‑1 (MQ=255)                                                  gatgTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACAGGTCCATCCCCTTTTCACCTGCTTCAAGatatt                                                                             <  1:111201/136‑2 (MQ=255)                                                     gTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAaca                                                                                                              <  5:406604/103‑1 (MQ=255)                                                     gTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAaca                                                                                                              >  6:406604/1‑103 (MQ=255)                                                     gTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCAAGa                                                                                 <  8:156117/132‑1 (MQ=255)                                                     gTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCAAGATATAAtt                                                                          >  4:217563/1‑139 (MQ=255)                                                     gTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCAAGATATAAtt                                                                          >  8:30575/1‑139 (MQ=255)                                                     gTTTCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGCGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCAAGa                                                                                 >  7:156117/1‑132 (MQ=255)                                                        tCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCAAGata                                                                               <  8:371924/131‑1 (MQ=255)                                                        tCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCAAGata                                                                               >  7:371924/1‑131 (MQ=255)                                                        tCAAACGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCAAGATATAATTCAt                                                                       >  6:84648/1‑139 (MQ=255)                                                          caacgTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTATCACGTCCATCCCCTTTTCACCTGCTTCAAGATATAATTCATGa                                                                     <  6:60720/135‑1 (MQ=255)                                                          aaacGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCAAGATATAATTCATg                                                                      <  3:348342/138‑1 (MQ=255)                                                          aaacGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCAAGATATAATTCATg                                                                      <  3:32606/138‑1 (MQ=255)                                                          aaacGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCAAGATATAATTCATg                                                                      <  4:3391/138‑1 (MQ=255)                                                          aaacGTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCAAGATATAATTCATGa                                                                     <  3:313903/139‑1 (MQ=255)                                                           aacgTTTTTAGGGTTCGCTTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCAAGATATAATTCATGaa                                                                    <  8:234827/136‑1 (MQ=255)                                                            acgTTTTTAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCAAGATATAATTCATGAAg                                                                   <  4:378353/137‑1 (MQ=255)                                                                 tttAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTACCACGTCCATCCCCTTTTCACCTGCTTCAAGATATAATTCATGAAGgtgt                                                               >  4:280103/1‑138 (MQ=255)                                                                  ttATGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCAAGATATAATTCATGAAGGTGTa                                                              <  3:172714/138‑1 (MQ=255)                                                                  ttAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTGCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCAAGATATAATTCATGAAGGTGTa                                                              <  5:268044/138‑1 (MQ=255)                                                                   tAGGTTTCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCAAGATATAATTCATGAAGGTGTaa                                                             <  1:91311/138‑1 (MQ=255)                                                                        ttCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAAc                                                                                                                         >  8:377539/1‑73 (MQ=255)                                                                        ttCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAAc                                                                                                                         <  7:377539/73‑1 (MQ=255)                                                                         tCGATTATTTACGCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCAAGATATAATTCATGAAGGTGTAAGGGATc                                                       >  7:250078/1‑138 (MQ=255)                                                                                     gCCGAGAATGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCAAGATATAATTCATGAAGGTGTAAGGGATCAAGCACCTCGcc                                           >  5:189910/1‑138 (MQ=255)                                                                                           aaTGTCGGGTGTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCAAGATATAATTCATGAAGGTGTAAGGGATCAAGCACCTCGCCGATTAAc                                    <  3:424865/139‑1 (MQ=255)                                                                                                     gTGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCAAGATATAATTCATGAAGGTGTAAGGGATCAAGCACCTCGCCGATTAACAATATGGCAt                          >  5:445837/1‑139 (MQ=255)                                                                                                      tGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCAAGATATAATTCATGAAGGTGTAAGGGATCAAGCACCTCGCCGATTAACAATATGGCATc                         >  6:221426/1‑139 (MQ=255)                                                                                                      tGAACACTTTCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCAAGATATAATTCATGAAGGTGTAAGGGATCAAGCACCTCGCCGATTAACAATATGGCATc                         <  5:194119/139‑1 (MQ=255)                                                                                                             tttCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACATGCTTCAAGATATAATTCATGAAGGTGTAAGGGATCAAGCACCTCGCCGATTAACAATATGGCATCCGCTCCg                  >  7:158553/1‑139 (MQ=255)                                                                                                               tCAATATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCAAGATATAATTCATGAAGGTGTAAGGGATCAAGCACCTCGCCGATTAACAATATGGCATCCGCTCCGa                 <  3:122930/138‑1 (MQ=255)                                                                                                                 aaTATTTGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCAAGATATAATTCATGAAGGTGTAAGGGATCAAGCACCTCGCCGATTAACAATATGGCATCCGCTCCGAtt               <  6:445837/138‑1 (MQ=255)                                                                                                                     tttGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCAAGATATAATTCATGAAGGTGTAAGGGATCAAGCACCTCGCCGAt                                        >  6:75089/1‑109 (MQ=255)                                                                                                                     tttGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCAAGATATAATTCATGAAGGTGTAAGGGATCAAGCACCTCGCCGAt                                        <  5:75089/109‑1 (MQ=255)                                                                                                                       tGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCAAGATATAATTCATGAAGGTGTAAGGGATCAAGCCCCTCGCCGATTAACAATATGGCATCCGCGCCGATTcttctt         >  6:217120/1‑138 (MQ=255)                                                                                                                       tGTTCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCAAGATATAATTCATGAAGGTGTAAGGGATCAAGCACCTCGCCGATTAACAATATGGCATCCGCTCCGATTcttctt         >  3:357149/1‑138 (MQ=255)                                                                                                                         ttCTAGCGTTGATGCATCATGACCCTCCACTAACACGTCCATCCCCTTTTCACCTGCGTCAAGATATAATTCATGAAGGTGTAAGGGATCAAGCACCTCGCCGATTAACAATATGGCATCCGCTCCGATTCTTCTTGag      >  6:363723/1‑138 (MQ=255)                                                                                                                         ttCTAGCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCAAGATATAATTCATGAAGGTGTAAGGGATCAAGCACCTCGCCGATTAACAATATGGCATCCGCTCCGATTCTTCTTGa       <  1:53739/138‑1 (MQ=255)                                                                                                                              gCGTTGATGCATCATGAACCTCCACTAACACGTCCATCCCCTTTTCACCTGCTTCAAGATATAATTCATGAAGGTGTAAGGGATCAAGCACCTCGCCGATTAACAATATGGCATCCGCGCCGATTCTTCTTGATtcct  <  8:158553/138‑1 (MQ=255)                                                                                                                                    |                                                                                                                                    CAGTGATTCTTTCGGAGCAAGAGATGCGATTTGTTCTGTCTGCATGATAGAGGTTTCAAAAGTTTTTAGGTTTCGGTTGTTTATGCCGAGAATATCAGGCGTGAATACTTTCAATATTTGTTCTAGCGTTGTTGCATCATGAACCTCCACTAACACGTCCATCTCCTTTTCACCTGCTTCAAGATATAATTCATGAAGGTGTAAGGGATCAAGCACTTCGCCGATTAACAATATGGCATCCGCTCCGATTCTTCTTGATTCCT  >  CP006881/2192142‑2192404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑