Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,192,448	T→A	I102I (ATA→ATT)	U712_10985 ←	Indole‑3‑glycerol phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,192,448	0	T	A	100.0%	169.3 / NA	54	I102I (ATA→ATT)	U712_10985	Indole‑3‑glycerol phosphate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (29/25);  total (29/25)

ACCTGCTTCAAGATATAATTCATGAAGGTGTAAGGGATCAAGCACTTCGCCGATTAACAATATGGCATCCGCTCCGATTCTTCTTGATTCCTCCACTTGAAGAGAATCAATAATAAAATCTTTTCTGAGTACAGGTATCGAAACAGCGCGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAAGCGCATCTGCTTTCGCAGTTTCATAGTCTTTTGCAATCCGCACAGGTACAAAAT  >  CP006881/2192313‑2192578                                                                                                                                        |                                                                                                                                   ttcacctgcttcaagatataattcatgaaggtgtaagggatcaagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATc                                                                                                                                 <  8:130171‑M1/28‑1 (MQ=255)        gcttcaagatataattcatgaaggtgtaagggatcaagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAg                                                                                                                          <  8:213666‑M1/35‑1 (MQ=255)         cttcaagatataattcatgaaggtgtaagggatcaagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAgc                                                                                                                         <  3:416204‑M1/36‑1 (MQ=255)          agtcagatataattgatgaaggtgtaagtgatcaagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACagca                                                                                                                        >  5:41177‑M1/103‑138 (MQ=255)            caagatataattcatgaaggtgtaagggatcaagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACagca                                                                                                                        >  7:346490‑M1/101‑136 (MQ=255)            caagatataattcatgaaggtgtaagggatcaagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACagca                                                                                                                        >  3:108497‑M1/101‑136 (MQ=255)            caagatataattcatgaaggtgtaagggatcaagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACagca                                                                                                                        >  7:130171‑M1/101‑136 (MQ=255)            caagatataattcatgaaggtgtaagggatcaagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACagca                                                                                                                        >  1:426127‑M1/101‑136 (MQ=255)            caagatataattcatgaaggtgtaagggatcaagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACagca                                                                                                                        >  1:216373‑M1/101‑136 (MQ=255)                   taattcatgaaggtgtaagggatcaagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTAc                                                                                                                >  8:328462‑M1/94‑138 (MQ=255)                     attcatgaaggtgtaagggatcaagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGt                                                                                                              >  3:280103‑M1/92‑138 (MQ=255)                           gaaggtgtaagggatcaagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATa                                                                                                        <  8:219317‑M1/53‑1 (MQ=255)                             aggtgtaagggatcaagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATaaa                                                                                                      >  4:107800‑M1/84‑138 (MQ=255)                              ggtgtaagggatcaagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATa                                                                                                    >  7:213666‑M1/83‑139 (MQ=255)                               tAAGGGATCAAGCACCTCGCCGATTAACAATATGGCATCCGCACCGATTCATCTTGATTCCTCTACTTTAAGAGAATC‑‑‑AATAAAATCTTTTCTAAGTACAGGAATCGAAACAGAACGCTATACGACTGATAAATAgaat                                                                                                >  1:25867/1‑136 (MQ=255)                                  taagggatcaagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAg                                                                                                   >  6:191140‑M1/79‑136 (MQ=255)                                  taagggatcaagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAg                                                                                                   <  5:191140‑M1/58‑1 (MQ=255)                                         tcaagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTccc                                                                                         >  8:285049‑M1/72‑139 (MQ=255)                                         tcaagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTccc                                                                                         <  3:380468‑M1/68‑1 (MQ=255)                                         tcaagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTccc                                                                                         <  2:187186‑M1/68‑1 (MQ=255)                                          caagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTAc                                                                                                                <  6:425663‑M1/45‑1 (MQ=255)                                          caagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTAc                                                                                                                >  5:425663‑M1/71‑115 (MQ=255)                                          caagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTcccc                                                                                        <  6:411810‑M1/69‑1 (MQ=255)                                                   cgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGaaaaaa                                                                               <  3:198015‑M1/78‑1 (MQ=255)                                                   cgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTAAAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGataaaa                                                                               <  6:165706‑M1/78‑1 (MQ=255)                                                         ttaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACagca                                                                                                                        >  7:201554‑M1/56‑91 (MQ=255)                                                         ttaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACagca                                                                                                                        <  8:201554‑M1/37‑2 (MQ=255)                                                            acaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTc                                                                      >  4:380468‑M1/53‑139 (MQ=255)                                                                  tggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTa                                                                 <  3:334962‑M1/92‑1 (MQ=255)                                                                       tccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACg                                                            <  1:150623‑M1/97‑1 (MQ=255)                                                                        ccgctcctattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAACCGGGGTGTCTGTTAAAACgg                                                           <  8:365410‑M1/98‑1 (MQ=255)                                                                        ccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACgg                                                           <  8:305919‑M1/98‑1 (MQ=255)                                                                          gctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGaaa                                                        <  3:224844‑M1/101‑1 (MQ=255)                                                                           cGATTCTTCTTGATTCCTCTACTTGAAGAGAATC‑‑‑AATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACCCTTTACGGCTGATAAATAGCTTTTTTCCCCTTGAAAAAACGGGGTGTCTTGTAAAACGGaaatct                                                     >  6:291114/1‑135 (MQ=255)                                                                                          attcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCg                                         >  5:291114‑M1/23‑138 (MQ=255)                                                                                          attcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCg                                         >  4:334962‑M1/23‑138 (MQ=255)                                                                                             cctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAg                                      >  7:361451‑M1/20‑138 (MQ=255)                                                                                                   cttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATa                               >  2:150623‑M1/14‑139 (MQ=255)                                                                                                          agaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACACCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCAGCTGCCTTCGCAGCCTCATAGTCtttt                        >  5:84648‑M1/7‑139 (MQ=255)                                                                                                           gaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTg                       >  1:290927‑M1/6‑139 (MQ=255)                                                                                                             atcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCg                                         >  2:403334‑M1/4‑119 (MQ=255)                                                                                                             atcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCg                                         <  1:403334‑M1/116‑1 (MQ=255)                                                                                                             atcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCTTAGTCTTTTGCa                     >  2:25867‑M1/4‑139 (MQ=255)                                                                                                             atcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCa                     >  8:91228‑M1/4‑139 (MQ=255)                                                                                                                  TAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCAt                                                  <  4:131130‑M1/105‑1 (MQ=255)                                                                                                                  TAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCAt                                                  >  3:131130‑M1/1‑105 (MQ=255)                                                                                                                    gaaTCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGTTAAATAGCTGTTTTGCCCTTGAATAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATctgga              <  3:165207/138‑5 (MQ=255)                                                                                                                    AAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCa              >  5:411810‑M1/1‑139 (MQ=255)                                                                                                                     aaTCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGcac             <  7:219317/139‑1 (MQ=255)                                                                                                                      aTCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGca              >  4:23488/1‑137 (MQ=255)                                                                                                                           ttCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAg           <  7:305919/135‑1 (MQ=255)                                                                                                                           ttCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAg           <  5:363723/135‑1 (MQ=255)                                                                                                                                aGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACaaa    <  3:217563/137‑1 (MQ=255)                                                                                                                                aGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAAt  <  1:200015/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                   ACCTGCTTCAAGATATAATTCATGAAGGTGTAAGGGATCAAGCACTTCGCCGATTAACAATATGGCATCCGCTCCGATTCTTCTTGATTCCTCCACTTGAAGAGAATCAATAATAAAATCTTTTCTGAGTACAGGTATCGAAACAGCGCGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAAGCGCATCTGCTTTCGCAGTTTCATAGTCTTTTGCAATCCGCACAGGTACAAAAT  >  CP006881/2192313‑2192578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑