Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,192,460	G→A	R98R (CGC→CGT)	U712_10985 ←	Indole‑3‑glycerol phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,192,460	0	G	A	100.0%	160.2 / NA	51	R98R (CGC→CGT)	U712_10985	Indole‑3‑glycerol phosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/25);  total (26/25)

TTCATGAAGGTGTAAGGGATCAAGCACTTCGCCGATTAACAATATGGCATCCGCTCCGATTCTTCTTGATTCCTCCACTTGAAGAGAATCAATAATAAAATCTTTTCTGAGTACAGGTATCGAAACAGCGCGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAAGCGCATCTGCTTTCGCAGTTTCATAGTCTTTTGCAATCCGCACAGGTACAAAATTCTCTTTAATAA  >  CP006881/2192331‑2192590                                                                                                                                  |                                                                                                                                   taattcatgaaggtgtaagggatcaagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTAc                                                                                                                            >  8:328462‑M1/94‑138 (MQ=255)   attcatgaaggtgtaagggatcaagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGt                                                                                                                          >  3:280103‑M1/92‑138 (MQ=255)         gaaggtgtaagggatcaagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATa                                                                                                                    <  8:219317‑M1/53‑1 (MQ=255)           aggtgtaagggatcaagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATaaa                                                                                                                  >  4:107800‑M1/84‑138 (MQ=255)            ggtgtaagggatcaagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATa                                                                                                                >  7:213666‑M1/83‑139 (MQ=255)             tAAGGGATCAAGCACCTCGCCGATTAACAATATGGCATCCGCACCGATTCATCTTGATTCCTCTACTTTAAGAGAATC‑‑‑AATAAAATCTTTTCTAAGTACAGGAATCGAAACAGAACGCTATACGACTGATAAATAgaat                                                                                                            >  1:25867/1‑136 (MQ=255)                taagggatcaagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAg                                                                                                               <  5:191140‑M1/58‑1 (MQ=255)                taagggatcaagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAg                                                                                                               >  6:191140‑M1/79‑136 (MQ=255)                       tcaagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTccc                                                                                                     >  8:285049‑M1/72‑139 (MQ=255)                       tcaagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTccc                                                                                                     <  2:187186‑M1/68‑1 (MQ=255)                       tcaagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTccc                                                                                                     <  3:380468‑M1/68‑1 (MQ=255)                        caagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTAc                                                                                                                            <  6:425663‑M1/45‑1 (MQ=255)                        caagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTAc                                                                                                                            >  5:425663‑M1/71‑115 (MQ=255)                        caagcacctcgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTcccc                                                                                                    <  6:411810‑M1/69‑1 (MQ=255)                                 cgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGaaaaaa                                                                                           <  3:198015‑M1/78‑1 (MQ=255)                                 cgccgattaacaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTAAAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGataaaa                                                                                           <  6:165706‑M1/78‑1 (MQ=255)                                          acaatatggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTc                                                                                  >  4:380468‑M1/53‑139 (MQ=255)                                                tggcatccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTa                                                                             <  3:334962‑M1/92‑1 (MQ=255)                                                     tccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACg                                                                        <  1:150623‑M1/97‑1 (MQ=255)                                                      ccgctcctattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAACCGGGGTGTCTGTTAAAACgg                                                                       <  8:365410‑M1/98‑1 (MQ=255)                                                      ccgctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACgg                                                                       <  8:305919‑M1/98‑1 (MQ=255)                                                        gctccgattcttcttgattcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGaaa                                                                    <  3:224844‑M1/101‑1 (MQ=255)                                                         cGATTCTTCTTGATTCCTCTACTTGAAGAGAATC‑‑‑AATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACCCTTTACGGCTGATAAATAGCTTTTTTCCCCTTGAAAAAACGGGGTGTCTTGTAAAACGGaaatct                                                                 >  6:291114/1‑135 (MQ=255)                                                                        attcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCg                                                     >  4:334962‑M1/23‑138 (MQ=255)                                                                        attcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCg                                                     >  5:291114‑M1/23‑138 (MQ=255)                                                                           cctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAg                                                  >  7:361451‑M1/20‑138 (MQ=255)                                                                                 cttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATa                                           >  2:150623‑M1/14‑139 (MQ=255)                                                                                        agaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACACCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCAGCTGCCTTCGCAGCCTCATAGTCtttt                                    >  5:84648‑M1/7‑139 (MQ=255)                                                                                         gaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTg                                   >  1:290927‑M1/6‑139 (MQ=255)                                                                                           atcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCg                                                     <  1:403334‑M1/116‑1 (MQ=255)                                                                                           atcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCg                                                     >  2:403334‑M1/4‑119 (MQ=255)                                                                                           atcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCTTAGTCTTTTGCa                                 >  2:25867‑M1/4‑139 (MQ=255)                                                                                           atcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCa                                 >  8:91228‑M1/4‑139 (MQ=255)                                                                                                TAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCAt                                                              >  3:131130‑M1/1‑105 (MQ=255)                                                                                                TAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCAt                                                              <  4:131130‑M1/105‑1 (MQ=255)                                                                                                  gaaTCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGTTAAATAGCTGTTTTGCCCTTGAATAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATctgga                          <  3:165207/138‑5 (MQ=255)                                                                                                  AAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCa                          >  5:411810‑M1/1‑139 (MQ=255)                                                                                                   aaTCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGcac                         <  7:219317/139‑1 (MQ=255)                                                                                                    aTCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGca                          >  4:23488/1‑137 (MQ=255)                                                                                                         ttCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAg                       <  5:363723/135‑1 (MQ=255)                                                                                                         ttCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAg                       <  7:305919/135‑1 (MQ=255)                                                                                                              aGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACaaa                <  3:217563/137‑1 (MQ=255)                                                                                                              aGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAAt              <  1:200015/139‑1 (MQ=255)                                                                                                                        tCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAAAGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTtaat    <  4:224844/139‑1 (MQ=255)                                                                                                                          gAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTc                                                                                  >  7:17543/1‑59 (MQ=255)                                                                                                                          gAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTc                                                                                  <  8:17543/59‑1 (MQ=255)                                                                                                                          gAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCa                                                   <  4:430137/90‑1 (MQ=255)                                                                                                                          gAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCa                                                   >  3:430137/1‑90 (MQ=255)                                                                                                                          gAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTtaataa  <  1:187186/139‑1 (MQ=255)                                                                                                                          gAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTtaat    >  6:230830/1‑137 (MQ=255)                                                                                                                          gAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCAAAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTtaat    >  6:122562/1‑137 (MQ=255)                                                                                                                                  |                                                                                                                                   TTCATGAAGGTGTAAGGGATCAAGCACTTCGCCGATTAACAATATGGCATCCGCTCCGATTCTTCTTGATTCCTCCACTTGAAGAGAATCAATAATAAAATCTTTTCTGAGTACAGGTATCGAAACAGCGCGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAAGCGCATCTGCTTTCGCAGTTTCATAGTCTTTTGCAATCCGCACAGGTACAAAATTCTCTTTAATAA  >  CP006881/2192331‑2192590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑