Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,192,525	G→T	L77I (CTT→ATT)	U712_10985 ←	Indole‑3‑glycerol phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,192,525	0	G	T	100.0%	144.8 / NA	47	L77I (CTT→ATT)	U712_10985	Indole‑3‑glycerol phosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (29/18);  total (29/18)

CCTCCACTTGAAGAGAATCAATAATAAAATCTTTTCTGAGTACAGGTATCGAAACAGCGCGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAAGCGCATCTGCTTTCGCAGTTTCATAGTCTTTTGCAATCCGCACAGGTACAAAATTCTCTTTAATAAGCCCTTTTGACGGCGACGCTTTCTTCACTTCAGCAATCAACCCGATAAACCGATTCAGGCTTGCCAGCGC  >  CP006881/2192402‑2192660                                                                                                                            |                                                                                                                                        attcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCg                                                                                                                           >  4:334962‑M1/23‑138 (MQ=255) attcctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCg                                                                                                                           >  5:291114‑M1/23‑138 (MQ=255)    cctctacttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAg                                                                                                                        >  7:361451‑M1/20‑138 (MQ=255)          cttgaagagaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATa                                                                                                                 >  2:150623‑M1/14‑139 (MQ=255)                 agaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACACCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCAGCTGCCTTCGCAGCCTCATAGTCtttt                                                                                                          >  5:84648‑M1/7‑139 (MQ=255)                  gaatcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTg                                                                                                         >  1:290927‑M1/6‑139 (MQ=255)                    atcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCg                                                                                                                           <  1:403334‑M1/116‑1 (MQ=255)                    atcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCg                                                                                                                           >  2:403334‑M1/4‑119 (MQ=255)                    atcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCTTAGTCTTTTGCa                                                                                                       >  2:25867‑M1/4‑139 (MQ=255)                    atcAATAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCa                                                                                                       >  8:91228‑M1/4‑139 (MQ=255)                         TAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCAt                                                                                                                                    >  3:131130‑M1/1‑105 (MQ=255)                         TAAAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCAt                                                                                                                                    <  4:131130‑M1/105‑1 (MQ=255)                           gaaTCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGTTAAATAGCTGTTTTGCCCTTGAATAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATctgga                                                                                                <  3:165207/138‑5 (MQ=255)                           AAATCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCa                                                                                                >  5:411810‑M1/1‑139 (MQ=255)                            aaTCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGcac                                                                                               <  7:219317/139‑1 (MQ=255)                             aTCTTTTCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGca                                                                                                >  4:23488/1‑137 (MQ=255)                                  ttCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAg                                                                                             <  5:363723/135‑1 (MQ=255)                                  ttCTAAGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAg                                                                                             <  7:305919/135‑1 (MQ=255)                                       aGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACaaa                                                                                      <  3:217563/137‑1 (MQ=255)                                       aGTACAGGAATCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAAt                                                                                    <  1:200015/139‑1 (MQ=255)                                                 tCGAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAAAGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTtaat                                                                          <  4:224844/139‑1 (MQ=255)                                                   gAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCa                                                                                                                         >  3:430137/1‑90 (MQ=255)                                                   gAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCa                                                                                                                         <  4:430137/90‑1 (MQ=255)                                                   gAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTtaataa                                                                        <  1:187186/139‑1 (MQ=255)                                                   gAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTtaat                                                                          >  6:230830/1‑137 (MQ=255)                                                   gAAACAGCACGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCAAAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTtaat                                                                          >  6:122562/1‑137 (MQ=255)                                                          cacgcTTTACTTATGATAAATAGCTGTATTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATAGAATCTGCCTTCGCCGACTCATAGTCTTATGCAATATGCACAGGTACATAATACACTATAATAAGCCCttt                                                                 >  4:204546/3‑139 (MQ=255)                                                          cacgcTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCttt                                                                 >  7:318044/3‑139 (MQ=255)                                                          cacgcTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCttt                                                                 >  7:199455/3‑139 (MQ=255)                                                               tttACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATc                                                                                                    <  7:192027/99‑1 (MQ=255)                                                               tttACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATc                                                                                                    >  8:192027/1‑99 (MQ=255)                                                               tttACGTCTGATAAATAGCTGTTTTCCACTTGAAAAAACGGGGTGTCTGTTAAAACGGACATCGCATCTGACTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACg                                                            >  8:210062/1‑139 (MQ=255)                                                                         aTAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCttt                                                  >  7:173472/1‑139 (MQ=255)                                                                         aTAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCttt                                                  >  1:369375/1‑139 (MQ=255)                                                                         aTAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCttt                                                  >  1:311186/1‑139 (MQ=255)                                                                         aTAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCttt                                                  >  7:9858/1‑139 (MQ=255)                                                                         aTAAATAGATGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATACGCCCGTTTGACGTCGATCCttt                                                  >  8:122583/1‑139 (MQ=255)                                                                                    ttttCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCa                                                                                                       >  6:193158/1‑75 (MQ=255)                                                                                    ttttCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCa                                                                                                       <  5:193158/75‑1 (MQ=255)                                                                                           cTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCaa                                <  5:217120/139‑1 (MQ=255)                                                                                              gAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGCCGGCGATGCTTTCTTCACTTCGGCAATCAAccc                             >  5:67261/1‑139 (MQ=255)                                                                                              gAAAAAACGGGGTGTCTGGTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAAc                               <  3:23488/137‑1 (MQ=255)                                                                                                         gtgtCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATcctc                                                                                >  7:239827/1‑77 (MQ=255)                                                                                                         gtgtCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATcctc                                                                                <  8:239827/77‑1 (MQ=255)                                                                                                                        gAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCACCCCGATAAACCGATTCGGGCTTGCCAgcg   >  8:175337/1‑139 (MQ=255)                                                                                                                         aaaTCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAgcgc  <  2:369375/139‑1 (MQ=255)                                                                                                                         aaaTCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAgcgc  <  5:165706/139‑1 (MQ=255)                                                                                                                            |                                                                                                                                        CCTCCACTTGAAGAGAATCAATAATAAAATCTTTTCTGAGTACAGGTATCGAAACAGCGCGCTTTACGTCTGATAAATAGCTGTTTTCCCCTTGAAAAAACGGGGTGTCTGTTAAAACGGAAAGCGCATCTGCTTTCGCAGTTTCATAGTCTTTTGCAATCCGCACAGGTACAAAATTCTCTTTAATAAGCCCTTTTGACGGCGACGCTTTCTTCACTTCAGCAATCAACCCGATAAACCGATTCAGGCTTGCCAGCGC  >  CP006881/2192402‑2192660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑