| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 2,192,622 | A→G | A44A (GCT→GCC) | U712_10985 ← | Indole‑3‑glycerol phosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,192,622 | 0 | A | G | 98.2% | 180.3 / NA | 57 | A44A (GCT→GCC) | U712_10985 | Indole‑3‑glycerol phosphate synthase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (35/21); minor base T (1/0); total (36/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTTGAAAAAACGGGGTGTCTGTTAAAACGGAAAGCGCATCTGCTTTCGCAGTTTCATAGTCTTTTGCAATCCGCACAGGTACAAAATTCTCTTTAATAAGCCCTTTTGACGGCGACGCTTTCTTCACTTCAGCAATCAACCCGATAAACCGATTCAGGCTTGCCAGCGCCTCTTTAAATGAACGTTTTTCGAAAGGCTGCTCTTCCGGCAAGACCAGTGTTTTTACTTCTTCTTTCTTTTGTTTAATGATGTTTTCAAGCAT > CP006881/2192492‑2192753 | cTTGAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCaa < 5:217120/139‑1 (MQ=255) gAAAAAACGGGGTGTCTGTTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGCCGGCGATGCTTTCTTCACTTCGGCAATCAAccc > 5:67261/1‑139 (MQ=255) gAAAAAACGGGGTGTCTGGTAAAACGGAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAAc < 3:23488/137‑1 (MQ=255) gAAATCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCACCCCGATAAACCGATTCGGGCTTGCCAgcg > 8:175337/1‑139 (MQ=255) aaaTCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAgcgc < 5:165706/139‑1 (MQ=255) aaaTCGCATCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAgcgc < 2:369375/139‑1 (MQ=255) tCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTa > 8:214390/1‑138 (MQ=255) tCTGCCTTCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTa > 7:140776/1‑138 (MQ=255) ttCGCAGCCTCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAAc > 7:233413/1‑139 (MQ=255) gcagcctCATAGTCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGt < 4:198015/132‑1 (MQ=255) tCTTTTGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGgct > 4:88134/1‑139 (MQ=255) ttttGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGgctgc > 3:126848/1‑139 (MQ=255) ttttGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCATTTTGACGGAGATGCTTTCTTCAATTCTGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTACTTAAATGAACTTTTATCGAAACgctg > 7:307690/1‑138 (MQ=255) tttGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGgctgct > 1:271100/1‑139 (MQ=255) ttGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAAGGTTTCTCGAAAGGATGctc > 4:310574/1‑139 (MQ=255) ttGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCg < 8:430260/128‑1 (MQ=255) ttGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCg > 7:430260/1‑128 (MQ=255) ttGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGctc > 1:293219/1‑139 (MQ=255) ttGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGctc > 1:193408/1‑139 (MQ=255) ttGAAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGgctgct > 8:314101/1‑138 (MQ=255) tGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGAt < 6:375884/89‑1 (MQ=255) tGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGAt > 5:375884/1‑89 (MQ=255) tGCAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGctc > 7:267651/1‑138 (MQ=255) cAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGctcta < 7:365410/138‑2 (MQ=255) cAATCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGctcta < 5:122562/138‑2 (MQ=255) aTCTGCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGCTGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCg > 3:231666/1‑139 (MQ=255) tctgCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCgg > 5:372998/4‑139 (MQ=255) tctgCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACACGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCgg > 3:239125/4‑139 (MQ=255) tgCACAGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCgg > 1:33513/2‑137 (MQ=255) cacaGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCgg < 8:140776/135‑1 (MQ=255) cacaGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCaga > 6:158712/1‑137 (MQ=255) aGGTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCagaacc > 2:291527/1‑134 (MQ=255) ggTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCa < 2:193408/139‑1 (MQ=255) ggTACAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCa < 2:293219/139‑1 (MQ=255) cAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGaa > 1:341948/1‑101 (MQ=255) cAAAATCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGaa < 2:341948/101‑1 (MQ=255) aaaaTCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGtt < 8:173472/139‑1 (MQ=255) aaaTCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAgcgc < 4:195093/86‑1 (MQ=255) aaaTCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAgcgc > 3:195093/1‑86 (MQ=255) aaaTCCTCTTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGttt > 1:27177/1‑139 (MQ=255) ctctTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCaa > 3:143194/1‑51 (MQ=38) ctctTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCaa < 4:143194/51‑1 (MQ=38) ctctTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCAct > 8:142133/1‑139 (MQ=255) ctctTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCAct > 5:413382/1‑139 (MQ=255) ctctTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTAGCAAGGCTGCTCTACCGGCAGAACCAGTGttttccct > 5:391714/1‑135 (MQ=255) ctctTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATACGGGCTTGCAACCGCCTCATTAAATGAACGTTTCTCGAAAGCCTGCTCTACCGGCAGAACCACGGtttacact > 5:6952/1‑134 (MQ=255) tctTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCActt > 2:234921/1‑139 (MQ=255) ttttATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCActt < 8:307690/137‑1 (MQ=255) tttAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCActt < 8:233413/137‑1 (MQ=255) aGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATaaa < 7:420622/50‑1 (MQ=255) aGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATaaa > 8:420622/1‑50 (MQ=255) ttttGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGt > 3:385281/1‑139 (MQ=255) ggCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTtgatga > 6:98294/1‑139 (MQ=255) ggCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTtgatga > 4:170491/1‑139 (MQ=255) gatgCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTgatgattt > 2:321489/4‑137 (MQ=255) cTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGATTTTTTc < 1:321489/139‑1 (MQ=255) cTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGATTTTTTc < 3:88134/139‑1 (MQ=255) cttcacttcgGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGATTTTTTCAAg < 2:27177/138‑1 (MQ=255) cacttcgGCAATCAACCCGCTAAACCGATTCGGGCTTGACAGCGACTCCTTAAATGTACGTTTCTCGAAAGGCTGCTCTCCCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTTATGATTTTTTCAAGCa < 7:122583/137‑1 (MQ=255) cacttcgGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGATTTTTTCAAGCa < 1:291527/137‑1 (MQ=255) cacttcgGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGATTTTTTCAAGCAt < 4:385281/138‑1 (MQ=255) | CTTGAAAAAACGGGGTGTCTGTTAAAACGGAAAGCGCATCTGCTTTCGCAGTTTCATAGTCTTTTGCAATCCGCACAGGTACAAAATTCTCTTTAATAAGCCCTTTTGACGGCGACGCTTTCTTCACTTCAGCAATCAACCCGATAAACCGATTCAGGCTTGCCAGCGCCTCTTTAAATGAACGTTTTTCGAAAGGCTGCTCTTCCGGCAAGACCAGTGTTTTTACTTCTTCTTTCTTTTGTTTAATGATGTTTTCAAGCAT > CP006881/2192492‑2192753 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |