Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,192,715	T→C	V13V (GTA→GTG)	U712_10985 ←	Indole‑3‑glycerol phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,192,715	0	T	C	100.0%	186.8 / NA	58	V13V (GTA→GTG)	U712_10985	Indole‑3‑glycerol phosphate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/35);  total (23/35)

CTCTTTAATAAGCCCTTTTGACGGCGACGCTTTCTTCACTTCAGCAATCAACCCGATAAACCGATTCAGGCTTGCCAGCGCCTCTTTAAATGAACGTTTTTCGAAAGGCTGCTCTTCCGGCAAGACCAGTGTTTTTACTTCTTCTTTCTTTTGTTTAATGA‑TGTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTGCGCGGCAGCGCCTCCGCTTGTAATCGTCTCTAATGCTAGCTCCGTTCCT  >  CP006881/2192580‑2192839                                                                                                                                        |                                                                                                                              ctctTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCAct                                                                                                                            >  8:142133/1‑139 (MQ=255) ctctTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCAct                                                                                                                            >  5:413382/1‑139 (MQ=255)  tctTTAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCActt                                                                                                                           >  2:234921/1‑139 (MQ=255)    ttttATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCActt                                                                                                                           <  8:307690/137‑1 (MQ=255)    tttAATAAGCCCTTTTGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCActt                                                                                                                           <  8:233413/137‑1 (MQ=255)                ttttGACGGCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGt                                                                                                             >  3:385281/1‑139 (MQ=255)                       ggCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTtgatga                                                                                                      >  4:170491/1‑139 (MQ=255)                       ggCGATGCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTtgatga                                                                                                      >  6:98294/1‑139 (MQ=255)                          gatgCTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTgatga‑ttt                                                                                                  >  2:321489/4‑137 (MQ=255)                              cTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTc                                                                                              <  3:88134/139‑1 (MQ=255)                              cTTTCTTCACTTCGGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTc                                                                                              <  1:321489/139‑1 (MQ=255)                                  cttcacttcgGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAg                                                                                           <  2:27177/138‑1 (MQ=255)                                     cacttcgGCAATCAACCCGCTAAACCGATTCGGGCTTGACAGCGACTCCTTAAATGTACGTTTCTCGAAAGGCTGCTCTCCCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTTATGA‑TTTTTTCAAGCa                                                                                         <  7:122583/137‑1 (MQ=255)                                     cacttcgGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCa                                                                                         <  1:291527/137‑1 (MQ=255)                                     cacttcgGCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCAt                                                                                        <  4:385281/138‑1 (MQ=255)                                            gCAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATctct                                                                                <  8:318044/139‑1 (MQ=255)                                             cAATCAACCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCtt                                                                               <  7:314101/139‑1 (MQ=255)                                                  aaCCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCctct                                                                          >  5:147555/1‑139 (MQ=255)                                                   aCCCGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCtt                                                                         <  4:126848/139‑1 (MQ=255)                                                     ccGATAAACCGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTGTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTc                                                                       <  2:311186/139‑1 (MQ=255)                                                              cGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGt                                                              <  6:6952/139‑1 (MQ=255)                                                              cGATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGt                                                              <  6:67261/139‑1 (MQ=255)                                                               gATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCttt                                                                        <  5:326030/128‑1 (MQ=255)                                                               gATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTc                                                             >  1:196190/1‑139 (MQ=255)                                                               gATTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTATTCCTCttt                                                                        >  6:326030/1‑128 (MQ=255)                                                                aTTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCa                                                                                         >  8:137127/1‑110 (MQ=255)                                                                aTTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCa                                                                                         >  4:117114/1‑110 (MQ=255)                                                                aTTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCa                                                                                         <  3:117114/110‑1 (MQ=255)                                                                aTTCGGGCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCa                                                                                         <  7:137127/110‑1 (MQ=255)                                                                     ggCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCa                                                        >  6:83135/1‑138 (MQ=255)                                                                      gCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAg                                                      <  7:214390/139‑1 (MQ=255)                                                                      gCTTGCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGGCGTTCAAg                                                      <  7:142133/139‑1 (MQ=255)                                                                          gCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGATTTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTg                                                   >  4:135452/1‑139 (MQ=255)                                                                          gCCAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTgc                                                  >  6:218920/1‑139 (MQ=255)                                                                           ccAGCGCCTCCTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTgcg                                                 >  6:628/1‑139 (MQ=255)                                                                               cgcCTCCTTAACTGAACGTTTCGCGATAGGCTGCTCTACCGGCAGAACGAGTGTTTTCACTTCTGCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCTTAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTGCGCGGc                                             <  4:416204/139‑1 (MQ=255)                                                                                 cctcctTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTGTCTGCTTTAGTCGTTCAAGCTGCGCGGCAg                                           >  2:362189/1‑139 (MQ=255)                                                                                  ctccTTAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTGCGCGGCAgc                                          <  5:83135/139‑1 (MQ=255)                                                                                    ccttAAATGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTAATGCTTAGATATCTTCCTCGTTCTGCTTTAGTCGTTCAAGCTGCTCGGCAgcgc                                        >  5:67825/3‑139 (MQ=255)                                                                                     cttAAATGAACGTCTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTGCGCGGCAGCGcc                                       <  2:271100/138‑1 (MQ=255)                                                                                        aaaTGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTGCGCGGCAGCGCCt                                      <  6:413382/137‑1 (MQ=255)                                                                                        aaaTGAACGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTGCGCGGCAGCGCCTc                                     <  5:628/138‑1 (MQ=255)                                                                                               cGTTTCTCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATATCTTCCTCTTTCTGCTTTAGTCTTTCAAACTTCCCGGCAGCCCCACCGCttct                              >  2:436079/1‑136 (MQ=255)                                                                                                 tttctCGAAAGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGAGCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTGCGCGGCAGCGCCTCCGCTTGTAAt                           <  5:230830/139‑1 (MQ=255)                                                                                                        aaaGGCTGCTCTACCGGCAGATCCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTGTGCTTTAGTGTTTCATGCTGCGCGGCAGCGCCTCCGCTTGTAATCGTctct                    <  1:436079/139‑1 (MQ=255)                                                                                                        aaaGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTGCGCGGCAGCGCCTCCGCTTGTAATCGTctct                    <  6:147555/139‑1 (MQ=255)                                                                                                        aaaGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTGCGCGGCAGCGCCTCCGCTTGTAATCGTctct                    <  6:372998/139‑1 (MQ=255)                                                                                                         aaGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGATTTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTGCGCGGCAGCGCCTCCGCTTGTAATCGTctct                    <  3:135452/139‑1 (MQ=255)                                                                                                         aaGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCttt                                                                        >  1:1677/1‑86 (MQ=255)                                                                                                         aaGGCTGCTCTACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCttt                                                                        <  2:1677/86‑1 (MQ=255)                                                                                                                ctctACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCctct                                                                          >  4:432061/1‑77 (MQ=255)                                                                                                                ctctACCGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCctct                                                                          <  3:432061/77‑1 (MQ=255)                                                                                                                     ccGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTGCGCGGCAGCGCCTCCGCTTGTAATCGTCTCTAACGCCAGCt          <  8:199455/136‑1 (MQ=255)                                                                                                                     ccGGCAGAACCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTGCGCGGCAGCGCCTCCGCTTGTAATCGTCTCTAACGCCAGCTCCg       <  3:310574/139‑1 (MQ=255)                                                                                                                       ggcagaaCCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTGCGCGGCAGCGCCTCCGCTTGTAATCGTCTCTAACGCCAGCTCCGtt     >  1:26420/7‑139 (MQ=255)                                                                                                                          agaaCCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTGCGCGGCAGCGCCTCCGCTTGTACTCGTCTCTAACGCCAGCTCCGtgccg  >  4:395706/4‑138 (MQ=255)                                                                                                                          agaaCCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTGCGCGGCAGCGCCTCCGCTTGTAATCGTCTCTAACGCCAGCTCCGttcct  <  5:98294/136‑1 (MQ=255)                                                                                                                          agaaCCAGTGTTTTCACTTCTTCTTTCTTTTGTTTGATGA‑TTTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTGCGCGGCAGCGCCTCCGCTTGTAATCGTCTCTAACGCCAGCTCCGttcct  <  3:170491/136‑1 (MQ=255)                                                                                                                                        |                                                                                                                              CTCTTTAATAAGCCCTTTTGACGGCGACGCTTTCTTCACTTCAGCAATCAACCCGATAAACCGATTCAGGCTTGCCAGCGCCTCTTTAAATGAACGTTTTTCGAAAGGCTGCTCTTCCGGCAAGACCAGTGTTTTTACTTCTTCTTTCTTTTGTTTAATGA‑TGTTTTCAAGCATAGATCTCTTCCTCTTTCTGCTTTAGTCGTTCAAGCTGCGCGGCAGCGCCTCCGCTTGTAATCGTCTCTAATGCTAGCTCCGTTCCT  >  CP006881/2192580‑2192839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑