Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,192,968	T→A	S265S (TCA→TCT)	U712_10990 ←	Anthranilate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,192,968	0	T	A	100.0%	260.5 / NA	82	S265S (TCA→TCT)	U712_10990	Anthranilate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (42/40);  total (42/40)

TTCCTTCCTTCAGTGAGGCGGTGATCCCCGCCGTGTATATCGCAGCCCCTGCATTAAAAGCTGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGACAGGCACTCTCTTTTGGTGACTCCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAAGCTTCCGGTGAAACGGTATATTCCCGGCGCTCCCCGTCCTTTAATTCAATCACGTCGGTCGGTGCTGTAATTGAAAGCTCATC  >  CP006881/2192835‑2193096                                                                                                                                      |                                                                                                                                 ttccttccTTCAGTGAGGCGGTAATTCCCGCCGTGTAAATCGCAGCACCCGCATTAAAAGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACtg                                                                                                                             >  4:36355/1‑138 (MQ=255) ttccttccTTCAGTGAGGCGGTAATTCCCGCCGTGTAAATCGCAGCACCCGCATTAAAAGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACtg                                                                                                                             <  3:437795/139‑2 (MQ=255) ttccttccTTCAGTGAGGCGGTAATTCCCGCCGTGTAAATCGCAGCACCCGCATTAAAAGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACtg                                                                                                                             >  1:272768/1‑138 (MQ=255) ttccttccTTCAGTGAGGCGGTAATTCCCGCCGTGTAAATCGCAGCACCCGCATTAAAAGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACtg                                                                                                                             >  8:426537/1‑138 (MQ=255) ttccttccTTCAGTGAGGCGGTAATTCCCGCCGTGTAAATAGCAGCACCCGCATTAAAAGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACtg                                                                                                                             >  1:401667/1‑138 (MQ=255) ttccttcaTTCTTTTTGGCGGTAATTCCCGCCGTGTAAATCGCAGCACCCGAATTAAAAGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACtg                                                                                                                             <  2:401667/139‑2 (MQ=255) gtccatcctTCAGTGAGGCGGTAATTCCCGCCGTGTAAATCGCAGCACCCGCATTAAAAGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACtg                                                                                                                             >  6:425555/2‑138 (MQ=255)      tccttcAGTGAGGCGGTAATTCCCGCCGTGTAAATCGCAGCACCCGCATTAAAAGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCt                                                                                                                        >  8:279737/1‑139 (MQ=255)           cAGTGAGGCGGTAATTCCCGCCGTGTAAATCGCAGCACCCGCATTAAAAGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTaa                                                                                                                    <  5:357608/138‑1 (MQ=255)              tGAGGCGGTAATTCCCGCCGTGTAAATCGCAGCACCCGCATTAAAAGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTTTAAATCt                                                                                                                >  6:270172/1‑139 (MQ=255)                aGGCGGTAATTCCCGCCGTGTAAATCGCAGCACCCGCATTAAAAGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCtt                                                                                                               <  7:48770/138‑1 (MQ=255)                aGGCGGTAATTCCCGCCGTGTAAACCGCTGCTCCCTCATTAAAACCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCtt                                                                                                               <  5:250962/138‑1 (MQ=255)                 ggCGGTAATTCCCGCCGTGTAAATCGCAGCACCCGCATTAAAAGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTc                                                                                                              >  2:15858/1‑138 (MQ=255)                  gCGGTAATTCCCGCCGTGTAAATCGCAGCACCCGCATTAAAAGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACtg                                                                                                                             >  4:78896/1‑121 (MQ=255)                  gCGGTAATTCCCGCCGTGTAAATCGCAGCACCCGCATTAAAAGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACtg                                                                                                                             <  3:78896/122‑2 (MQ=255)                      taattcccGCCGTGTAAATCGCAGCCCCCGCATTAAAAGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGACACTGCACCGGTAAATCTTCAATTCt                                                                                                        >  6:359783/6‑139 (MQ=255)                              gccgTGTAAATCGCAGCACCCGCATTAAAAGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTtg                                                                                                >  8:72337/1‑139 (MQ=255)                                    taaatCGCAGCACCCGCATTAAAAGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAAc                                                                                          >  3:167324/4‑139 (MQ=255)                                      aatCGCAGCACCCGCATTAAAAGCCGAAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCg                                                                                        <  4:52571/138‑1 (MQ=255)                                       atCGCAGCACCCGCATTAAAAGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCg                                                                                        >  7:305600/1‑138 (MQ=255)                                       atCGCAGCACCCGCATTAAAAGCCGTAATAGATAAAGCGGAACT‑CTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGa                                                                                       >  3:167725/1‑138 (MQ=255)                                            aGCACCCGCATTAAAAGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCt                                                                                  >  5:130180/1‑139 (MQ=255)                                            aGCACCCGCATTAAAAGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCt                                                                                  >  3:62059/1‑139 (MQ=255)                                            aGCACCCGCATTAAAAGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCt                                                                                  >  7:190122/1‑139 (MQ=255)                                              cacccgCATTAAAAGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTc                                                                                >  6:24207/6‑139 (MQ=255)                                                   gaaTTAAAAGCGGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGTATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAAGCGAAATCTTCGGGTg                                                                           <  4:396990/137‑1 (MQ=255)                                                   gCATTAAAAGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTg                                                                           <  8:190122/139‑1 (MQ=255)                                                   gCATTAAAAGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTg                                                                           <  6:130180/139‑1 (MQ=255)                                                    cATTAAAAGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTg                                                                           >  1:25888/1‑138 (MQ=255)                                                      ttAAAAGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGaaa                                                                        <  5:107371/139‑1 (MQ=255)                                                      ttAAAAGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGaaa                                                                        <  4:167324/139‑1 (MQ=255)                                                      ttAAAAGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGaaa                                                                        <  3:447393/139‑1 (MQ=255)                                                      ttAAAAGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGaaa                                                                        <  5:359783/139‑1 (MQ=255)                                                      ttAAAAGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGaaa                                                                        <  2:25888/139‑1 (MQ=255)                                                      ttAAAAGACGTAATTGATAAAGCGGTACTGCTGATTTTATTTTAAAAAATATTATGAATGAGATAAGCGCTCTCTTTCGGAGACTTCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGaaa                                                                        <  4:441631/139‑1 (MQ=255)                                                        aaaaGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATGTGTGAAACCGAAATCTTCGGGTGAAACg                                                                      >  1:177172/1‑139 (MQ=255)                                                          aaGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACgg                                                                     >  8:266574/1‑138 (MQ=255)                                                           aGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTtgtg                                                                                              >  4:20959/1‑112 (MQ=255)                                                           aGCCGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTtgtg                                                                                              <  3:20959/112‑1 (MQ=255)                                                            gccgTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGtat                                                                  <  5:24207/136‑1 (MQ=255)                                                               gTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGtatact                                                               >  6:398141/1‑137 (MQ=255)                                                                       tAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGACATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGgcgc                                                       >  3:346798/1‑139 (MQ=255)                                                                       tAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGT‑AATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCt                                                      <  7:121676/139‑1 (MQ=255)                                                                        aaaGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGaaa                                                                        <  5:239799/121‑1 (MQ=255)                                                                        aaaGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGaaa                                                                        >  6:239799/1‑121 (MQ=255)                                                                          aGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCTct                                                    >  3:162363/1‑138 (MQ=255)                                                                          aGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCTct                                                    >  6:435260/1‑138 (MQ=255)                                                                          aGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCTct                                                    >  1:410393/1‑138 (MQ=255)                                                                             ggAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCTCTCCg                                                 <  5:338272/139‑1 (MQ=255)                                                                             ggAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCTCTCCg                                                 <  5:340727/139‑1 (MQ=255)                                                                             ggAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCTCTCCg                                                 <  5:398141/139‑1 (MQ=255)                                                                               aaCTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGaaa                                                                        >  2:408190/1‑114 (MQ=255)                                                                               aaCTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGaaa                                                                        <  1:408190/114‑1 (MQ=255)                                                                               aaCTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCTCTCCGTc                                               >  7:63226/1‑139 (MQ=255)                                                                                    ctgctTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCTCTCCg                                                 <  2:208117/132‑1 (MQ=255)                                                                                    ctgctTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCTCTCCg                                                 >  1:208117/1‑132 (MQ=255)                                                                                    ctgctTTTATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCTCTCCGTCCttt                                           >  7:277397/1‑138 (MQ=255)                                                                                         tttATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGaaa                                                                        <  2:126039/104‑1 (MQ=255)                                                                                         tttATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGaaa                                                                        >  1:126039/1‑104 (MQ=255)                                                                                         tttATTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCTCTCCGTCCTTTAAtta                                      >  2:397039/1‑137 (MQ=255)                                                                                            aTTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCTCTCCGTCCTTTAATTCAAt                                   >  6:224801/1‑138 (MQ=255)                                                                                            aTTTTCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCTCTCCGTCCTTTAATTCAAt                                   <  8:63226/138‑1 (MQ=255)                                                                                              tttCAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCTCTCCGTCCTTTAATTCAATCAc                                >  1:28218/1‑139 (MQ=255)                                                                                                tCAAAAATATTCTGAATGCGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCTCTCCGTCCTTTAATTCAATCACg                               <  6:255380/138‑1 (MQ=255)                                                                                                 cAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCTCTCCGTCCTTTAATTCAATCACgtc                             >  6:315149/1‑139 (MQ=255)                                                                                                 cAAAAATATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCTCTCCGTCCTTTAATTCAATCACgt                              <  1:15858/138‑1 (MQ=255)                                                                                                    aaaTATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCTCTCCGTCCTTTAATTCAATCACgtcggt                          <  4:62059/139‑1 (MQ=255)                                                                                                    aaaTATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCTCTCCGTCCTTTAATTCAATCACgtcggt                          <  4:346798/139‑1 (MQ=255)                                                                                                    aaaTATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCTCTCCGTCCTTTAATTCAATCACgtcggt                          <  4:167725/139‑1 (MQ=255)                                                                                                    aaaTATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCTCTCCGTCCTTTAATTCAATCACgtcggt                          <  4:146004/139‑1 (MQ=255)                                                                                                    aaaTATTCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCTCTCCGTCCTTTAATTCAATCACgtcggt                          <  8:305600/139‑1 (MQ=255)                                                                                                         ttCTGAATGAGATAAGCGCTCTCTTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCTCTCCGTCCTTTAATTCAATCACGTCGGTCGGTg                     <  3:36355/139‑1 (MQ=255)                                                                                                                       agcgctctctTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCTCTCCGTCCTTTAATTCAATCACGTCGGTCGGTGCTGTAATTGAAAg        <  7:279737/134‑1 (MQ=255)                                                                                                                       agcgctctctTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCTCTCCGTCCTTTAATTCAATCACGTCGGTCGGTGCTGTAATTGAAAg        <  2:272768/134‑1 (MQ=255)                                                                                                                        gcgctctctTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCTCTCCGTCCTTTAATTCAATCACGTCGGTCGGTGCTGTAATTGAAAGCt      >  2:144212/4‑139 (MQ=255)                                                                                                                             ctctTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCTCTCCg                                                 >  6:95284/1‑91 (MQ=255)                                                                                                                             ctctTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCTCTCCg                                                 <  5:95284/91‑1 (MQ=255)                                                                                                                             ctctTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCTCTCCGTCCTTTAATTCAATCACGTCGGTCGGTGCTGTAATTGAAAg        <  7:439982/132‑1 (MQ=255)                                                                                                                             ctctTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCTCTCCGTCCTTTAATTCAATCACGTCGGTCGGTGCTGTAATTGAAAg        >  8:439982/1‑132 (MQ=255)                                                                                                                             ctctTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCGGCGCTCTCCGTCCTTTAATTCAATCACGTCGGTCGGTGCTGTAATTGAAAGCTCATc  >  2:250433/1‑138 (MQ=255)                                                                                                                              tctTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCgg                                                          >  2:352875/1‑81 (MQ=255)                                                                                                                              tctTTCGGAGACTGCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAATCTTCGGGTGAAACGGTATACTCCCgg                                                          <  1:352875/81‑1 (MQ=255)                                                                                                                                      |                                                                                                                                 TTCCTTCCTTCAGTGAGGCGGTGATCCCCGCCGTGTATATCGCAGCCCCTGCATTAAAAGCTGTAATAGATAAAGCGGAACTGCTGCTTTTATTTTCAAAAATATTCTGAATGAGACAGGCACTCTCTTTTGGTGACTCCACCTGTAAATCTTCAAGTCTGCCATTTGTGAAACCGAAAGCTTCCGGTGAAACGGTATATTCCCGGCGCTCCCCGTCCTTTAATTCAATCACGTCGGTCGGTGCTGTAATTGAAAGCTCATC  >  CP006881/2192835‑2193096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑