| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 2,173,649 | G→T | S33S (TCC→TCA) | U712_10870 ← | Bifunctional protein BirA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,173,649 | 0 | G | T | 98.2% | 172.4 / ‑3.8 | 56 | S33S (TCC→TCA) | U712_10870 | Bifunctional protein BirA |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (24/31); total (24/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01 | |||||||||||
TTCAATCCGAAGCGAATTTCACTTTCACTGAGTTTCCCGGGCTTTTTGATAAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAGCCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTGGAACAGCCGAGGGCATCACTGATTTTTTGGCCGGAAATAAATTCACTTCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCACTCCTCTATGTGTTTCTTTTGATTG > CP006881/2173515‑2173783 | tataaTCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGaa < 8:200186/135‑1 (MQ=255) aTCCAAAACGAATTTCGCTTTCACTGAGTTTTCCTGGTTTGTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTAATTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTCAACAGcc < 7:334692/139‑1 (MQ=255) aTCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGcc < 5:143087/139‑1 (MQ=255) aTCCAAAACGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGcc < 4:100291/139‑1 (MQ=255) ccaaaacGAATTTCGCTTTCACTGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAGCTGTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGa < 3:43951/133‑1 (MQ=255) tcactGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGaa > 1:137129/1‑114 (MQ=255) tcactGAGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGaa < 2:137129/114‑1 (MQ=255) aGTTTTCCGGGTTTTTTGATGATCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATCTGCTTCCACACAGCAGTTCTTTAACAGCCGAGAGCATAACTGATTTTTTGGCCg < 4:207217/138‑1 (MQ=255) aGTTTTCCGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCg < 3:378553/138‑1 (MQ=255) tccGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGaaat > 5:11534/2‑138 (MQ=255) ccGGGTTTTTTGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGACGGaaata > 8:442120/1‑138 (MQ=255) gttttttGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGc < 6:308341/124‑1 (MQ=255) gttttttGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGc > 5:308341/3‑126 (MQ=255) tttttGATGAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCat > 6:390276/1‑138 (MQ=255) ccGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCt > 8:227906/1‑137 (MQ=255) cTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAaataa < 8:285150/139‑1 (MQ=255) tttCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGaa > 6:270966/1‑75 (MQ=255) tttCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGaa < 5:270966/75‑1 (MQ=255) tttCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGcc > 7:257644/1‑80 (MQ=255) tttCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGcc < 8:257644/80‑1 (MQ=255) aCGGCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGt > 1:319228/1‑139 (MQ=255) ggCTTCTACTTCATAGCCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTGGAACAGCCGAGGGCATCACTGATTTTTTGGCCGGAAATAAATTCACTTCCGGCCTGAGAAAATAATTCAATAAGGTCt < 3:382274/139‑1 (MQ=255) ggCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCt < 5:390276/139‑1 (MQ=255) ttCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAaataa > 8:80501/1‑123 (MQ=255) ttCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAaataa < 7:80501/123‑1 (MQ=255) acccTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGAc < 7:206355/138‑1 (MQ=255) accatctTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGTCCTGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGAc < 8:126247/135‑1 (MQ=255) tttCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCa < 2:319228/137‑1 (MQ=255) tttCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCa < 7:3426/137‑1 (MQ=255) tttCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCa < 4:91896/137‑1 (MQ=255) tttCCGAAGCTCTTCAATATGCTTACACACAGCAGTTATTGAACAGCAGAGAGCTTCACTGATTTTTTGGCAGGAAATAAATTAATTGCCGTCCTGAGAAAATAATTCAATAAGGTCTTTTATTAATGTTGAACGCATg > 8:406845/1‑139 (MQ=255) tCCGAAGCTATTCAATATGCTTCCACACATCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGTAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCa < 7:406845/135‑1 (MQ=255) gAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTc < 4:128616/139‑1 (MQ=255) gAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTc < 7:227906/139‑1 (MQ=255) ttCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCa < 1:222276/137‑1 (MQ=255) ttCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCa < 6:11534/137‑1 (MQ=255) atGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCACTACTCTa < 4:79947/139‑1 (MQ=255) tGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCACTCCTCTAt > 2:376655/1‑139 (MQ=255) cTTCCACCCAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCACTCCTCTAtgt > 6:277644/1‑139 (MQ=255) ttCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTga > 5:319742/1‑75 (MQ=255) ttCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTga < 6:319742/75‑1 (MQ=255) ttCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCt < 6:392448/96‑1 (MQ=255) ttCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCt < 6:398894/96‑1 (MQ=255) ttCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCt > 5:398894/1‑96 (MQ=255) ttCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCt > 5:392448/1‑96 (MQ=255) ttCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGcc > 3:369216/1‑126 (MQ=255) ttCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGcc < 4:369216/126‑1 (MQ=255) cacacaGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCACTCCTCTATGTGttt > 2:86079/1‑139 (MQ=255) agcagTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATg > 3:29076/1‑98 (MQ=255) agcagTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATg < 4:29076/98‑1 (MQ=255) cagTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTc < 6:179963/90‑1 (MQ=255) cagTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTc > 5:179963/1‑90 (MQ=255) cagTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCACTCCTCTATGTGTTTCTTTTGa > 6:226415/1‑139 (MQ=255) cagTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCACTCCTCTATGTGTTTCTTTTGa > 1:115258/1‑139 (MQ=255) cagTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCACTCCTCTATGTGTTTCTTTTGa > 4:386832/1‑139 (MQ=255) ttCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCACTCCTCTATGTGTTTCTTTTGATTg > 2:429698/1‑139 (MQ=255) | TTCAATCCGAAGCGAATTTCACTTTCACTGAGTTTCCCGGGCTTTTTGATAAGCCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAGCCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTGGAACAGCCGAGGGCATCACTGATTTTTTGGCCGGAAATAAATTCACTTCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCACTCCTCTATGTGTTTCTTTTGATTG > CP006881/2173515‑2173783 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |