Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,193,280 C→T A161A (GCG→GCA)  U712_10990 ← Anthranilate phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,193,2800CT99.0% 297.6 / NA 95A161A (GCG→GCA) U712_10990Anthranilate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (47/47);  minor base G (1/0);  total (48/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCCAGTGCGCTTGCCATCAATCCAGCTTTTTCAGCGGAATAGACCCCAATCACCTGGCGCTTCGCCTGCAAGGGATTGCTAAGCGGTCCGAGCAAATTAAATACTGTTCTGAAACCCAGTTCTTTTCTTGTACCCGCTACATGTTTCATAGACGAATGATAGAGCGGTGCAAAAAGAAATCCCATGTTGTTTGCTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATTGGAACCTCTAACACCTCTAAAACATCAGCGCTTCC  >  CP006881/2193145‑2193414
                                                                                                                                       |                                                                                                                                      
ctcCAGTGCGCTTGCCATCAGTCCAGCTTTTTCAACAGAATAGACCCCAATCACCTGACGCTTCGCCTGAAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGc                                                                                                                                      >  7:137224/1‑138 (MQ=255)
      tGCGCTTGCCATCAGTCCAGCTTTTTCAACAGAATAGACCCCAATCACCTGACGCTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATATCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGt                                                                                                                               >  1:67890/1‑139 (MQ=255)
        cgcTTGCCATCAGTCCAGCTTTTTCAACAGAATAGACCCCAATCACCTGACGCTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAACCCTCCCTCTTGTCTAGTACCTCCTCCATggat                                                                                                                             >  6:298403/1‑136 (MQ=255)
          cTTGCCATCAGTCCAGCTTTTTCAACAGAATAGACCCCAATCACCTGACGCTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCa                                                                                                                           >  5:379140/1‑139 (MQ=255)
          cTTGCCATCAGTCCAGCTTTTTCAACAGAATAGACCCCAATCACCTGACGCTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCa                                                                                                                           <  4:183843/139‑1 (MQ=255)
           ttGCCATCAGTCCAGCTTTTTCAACAGAATAGACCCCAATCACCTGACGCTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCa                                                                                                                           >  7:281802/1‑138 (MQ=255)
           ttGCCATCAGTCCAGCTTTTTCAACAGAATAGACCCCAATCACCTGACGCTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCa                                                                                                                           >  8:229462/1‑138 (MQ=255)
              ccATCAGTCCAGCTTTTTCAACAGAATAGACCCCAATCACCTGACGCTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATTTTTCATAg                                                                                                                        <  4:158274/138‑1 (MQ=255)
              ccATCAGTCCAGCTTTTTCAACAGAATAGACCCCAATCACCTGACGCTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAg                                                                                                                        <  8:384687/138‑1 (MQ=255)
                aTCAGTCCAGCTTTTTCTACAGAATAGAACCCAATCACCTGACGCTTCGCCTGTAAATGATTGCTGAGCGGCCCAAGCAGATTAAATTCCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACg                                                                                                                     <  1:388993/139‑1 (MQ=255)
                aTCAGTCCAGCTTTTTCAACAGAATAGACCCCAATCACCTGACGCTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACg                                                                                                                     <  6:379140/139‑1 (MQ=255)
                aTCAGTCCAGCTTTTTCAACAGAATAGACCCCAATCACCTGACGCTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACg                                                                                                                     <  8:281802/139‑1 (MQ=255)
                aTCAGTCCAGCTTTTTCAACAGAAAAGACCCCAATCACCTGACGCTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACg                                                                                                                     <  2:177172/139‑1 (MQ=255)
                 tCAGTCCAGCTTTTTCAACAGAATAGACCCCAATCACCTGACGCTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGa                                                                                                                    <  5:308652/139‑1 (MQ=255)
                     tCCAGCTTTTTCAACAGAATAGACCCCAATCACCTGACGCTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATgg                                                                                                                >  3:125337/1‑138 (MQ=255)
                       cAGCTTTTTCAACAGAATAGACCCCAATCACCTGACGCTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATggt                                                                                                               >  6:192252/1‑136 (MQ=255)
                       cAGCTTTTTCAACAGAATAGACCCCAATCACCTGACGCTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATggt                                                                                                               >  8:268034/1‑136 (MQ=255)
                       cAGCTTTTTCAACAGAATAGACCCCAATCACCTGACGCTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTATAGTACCTGCTACATGATGAATAGACGAATggt                                                                                                               >  6:297642/1‑136 (MQ=255)
                        aGCTTTTTCAACAGAATAGACCCCAATCACCTGACGCTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCGGCTACATGTTTCATAGACGAATGGTa                                                                                                              >  2:27404/1‑138 (MQ=255)
                               tcaacagAATAGACCCCAATCACCTGACGCTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACg                                                                                                                     >  1:64883/7‑124 (MQ=255)
                               tcaacagAATAGACCCCAATCACCTGACGCTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACg                                                                                                                     <  2:64883/118‑1 (MQ=255)
                                 aacagAATAGACCCCAATCACCAGACGCTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCa                                                                                                    <  3:71795/135‑1 (MQ=255)
                                      aaTAGACCCCAATCACCTGACGCTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCaaa                                                                                                  >  6:20787/1‑136 (MQ=255)
                                      aaTAGACCCCAATCACCTGACGCTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAg                                                                                               <  7:268034/139‑1 (MQ=255)
                                      aaTAGACCCCAATCACCTGACGCTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAg                                                                                               <  3:227208/139‑1 (MQ=255)
                                       aTAGACCCCAATCACCTGACGCTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAg                                                                                               <  2:181402/138‑1 (MQ=255)
                                           aCCCCAATCACCTGACGCTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATc                                                                                          >  8:244028/1‑139 (MQ=255)
                                             cccATTGACGTGACGCTTCGCCTGTTAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATccc                                                                                        <  5:297642/139‑1 (MQ=255)
                                             cccAATCACCTGACGCTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATccc                                                                                        <  5:192252/139‑1 (MQ=255)
                                             cccAATCACCTGACGCTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATcc                                                                                         >  5:408425/1‑138 (MQ=255)
                                                caTCACCTGACGCTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCAtg                                                                                     <  2:395073/138‑1 (MQ=255)
                                                aaccACCTGACGCTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCAtg                                                                                     >  1:195402/4‑139 (MQ=255)
                                                aaTCACCTGACGCTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAACAAGAAATCCCAtg                                                                                     >  2:5743/1‑139 (MQ=255)
                                                     ccTGACGCTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATATCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATgttgtt                                                                                <  2:67890/139‑1 (MQ=255)
                                                       tgacgcTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTGTTCTAGTTCCTGCTACATGTTTCATAGACGAATGGTCAAGCGGCTCAACAAGAAATCCCATGTTGTTTg                                                                              >  6:14859/4‑139 (MQ=255)
                                                            cTTCGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTc                                                                          >  8:342795/1‑138 (MQ=255)
                                                               cGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAAtg                                                                      >  1:122548/1‑139 (MQ=255)
                                                               cGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAAtg                                                                      >  1:146003/1‑139 (MQ=255)
                                                               cGCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAAtg                                                                      >  8:323705/1‑139 (MQ=255)
                                                                gCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAAtgc                                                                     >  5:69973/1‑139 (MQ=255)
                                                                gCCTGTAAAGGATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAAtg                                                                      <  7:244028/138‑1 (MQ=255)
                                                                     taaaggATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCtt                                                                >  8:121797/5‑139 (MQ=255)
                                                                          gATTGCTGAGCGTCCCAAGCAGATTATATACCGATCTGAAACCTAGCTCTTTTATAGTACCTGCTACATGTTTAATATACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGAc                                                           <  5:14859/139‑1 (MQ=255)
                                                                          gATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGAc                                                           <  6:344634/139‑1 (MQ=255)
                                                                          gATTGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGGACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGGTGTTTGTTTCAATGCTGCTTTTGAc                                                           <  7:215951/139‑1 (MQ=255)
                                                                            ttGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAg                                                                                               >  5:203424/1‑101 (MQ=255)
                                                                            ttGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAg                                                                                               <  6:203424/101‑1 (MQ=255)
                                                                            ttGCTGAGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCt                                                         >  8:66000/1‑139 (MQ=255)
                                                                               ctgaGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGTCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCtttt                                                      >  2:256054/4‑139 (MQ=255)
                                                                               ctgaGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCtttt                                                      >  4:43144/4‑139 (MQ=255)
                                                                                  aGCGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGTCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCgg                                                   >  1:171647/1‑139 (MQ=255)
                                                                                    cGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGaa                                                                                             >  8:120628/1‑95 (MQ=255)
                                                                                    cGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGaa                                                                                             <  7:120628/95‑1 (MQ=255)
                                                                                    cGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCgg                                                   >  1:251356/1‑137 (MQ=255)
                                                                                    cGGCCCAAGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGa                                                 <  1:256054/139‑1 (MQ=255)
                                                                                         caaGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGt                                            <  7:121797/137‑1 (MQ=255)
                                                                                         caaGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACAGGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATTCTGCTTTTGACCTTTTCGGGAGTGGt                                            <  8:300767/137‑1 (MQ=255)
                                                                                           aGCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGttt                                          <  6:69973/139‑1 (MQ=255)
                                                                                            gCAGATTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGttt                                          >  8:358779/1‑138 (MQ=255)
                                                                                                aTTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGt                                            <  4:13170/132‑1 (MQ=255)
                                                                                                aTTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGt                                            >  3:13170/1‑132 (MQ=255)
                                                                                                aTTAAATACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGaa                                       >  1:34824/1‑137 (MQ=255)
                                                                                                   aaaTACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATTGGAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAAtaga                                   <  6:76534/138‑4 (MQ=255)
                                                                                                   aaaTACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAAtaga                                   <  2:54606/138‑4 (MQ=255)
                                                                                                   aaaTACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAAtaga                                   <  7:358779/138‑4 (MQ=255)
                                                                                                   aaaTACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAAtaga                                   <  7:229462/138‑4 (MQ=255)
                                                                                                   aaaTACCGTGCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAAtaga                                   <  6:83015/138‑4 (MQ=255)
                                                                                                     aTACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGAc                                                           <  3:228479/112‑1 (MQ=255)
                                                                                                     aTACCGTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGAc                                                           >  4:228479/1‑112 (MQ=255)
                                                                                                          gTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGa                                                 <  3:364979/117‑1 (MQ=255)
                                                                                                          gTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGa                                                 >  4:364979/1‑117 (MQ=255)
                                                                                                          gTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAAtaga                                   >  8:329690/1‑128 (MQ=255)
                                                                                                          gTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAAtaga                                   <  7:329690/131‑4 (MQ=255)
                                                                                                          gTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACctc                             >  5:361132/1‑137 (MQ=255)
                                                                                                          gTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACctc                             >  4:362184/1‑137 (MQ=255)
                                                                                                          gTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACctc                             >  4:443990/1‑137 (MQ=255)
                                                                                                          gTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACctc                             >  1:283061/1‑137 (MQ=255)
                                                                                                          gTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACctc                             >  8:22686/1‑137 (MQ=255)
                                                                                                           ttCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAAtgc                                                                     <  1:135766/96‑1 (MQ=255)
                                                                                                           ttCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAAtgc                                                                     >  2:135766/1‑96 (MQ=255)
                                                                                                           ttCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTag                          <  7:342795/139‑2 (MQ=255)
                                                                                                           ttCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTag                          <  8:137224/139‑2 (MQ=255)
                                                                                                             cTGAAACCTATCTCTTTTCTAGTACCTGCTACATGATTCAGAGACTAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTTCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTagc                         <  5:186090/138‑3 (MQ=255)
                                                                                                              tGACACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGGTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTagc                         <  8:167798/137‑3 (MQ=255)
                                                                                                                caaCCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGAGGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTagctcc                      <  1:131064/137‑6 (MQ=255)
                                                                                                                   cctagctcttttctaGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTa                  <  7:323705/133‑1 (MQ=255)
                                                                                                                     tagctcttttctaGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTaa                 >  8:132836/5‑138 (MQ=255)
                                                                                                                     tagctcttttctaGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTaa                 >  7:94185/5‑138 (MQ=255)
                                                                                                                      agctcttttctaGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTaaaa               >  6:396861/4‑139 (MQ=255)
                                                                                                                           ttttctaGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACAt            <  5:20787/137‑1 (MQ=255)
                                                                                                                             ttctaGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAAtgc                                                                     >  8:103034/1‑78 (MQ=255)
                                                                                                                             ttctaGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAAtgc                                                                     <  7:103034/78‑1 (MQ=255)
                                                                                                                                  gTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGa                                                 <  8:180649/93‑1 (MQ=255)
                                                                                                                                  gTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGa                                                 >  7:180649/1‑93 (MQ=255)
                                                                                                                                    aCCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTcc  <  2:283061/138‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
CTCCAGTGCGCTTGCCATCAATCCAGCTTTTTCAGCGGAATAGACCCCAATCACCTGGCGCTTCGCCTGCAAGGGATTGCTAAGCGGTCCGAGCAAATTAAATACTGTTCTGAAACCCAGTTCTTTTCTTGTACCCGCTACATGTTTCATAGACGAATGATAGAGCGGTGCAAAAAGAAATCCCATGTTGTTTGCTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATTGGAACCTCTAACACCTCTAAAACATCAGCGCTTCC  >  CP006881/2193145‑2193414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑