Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,193,379	T→A	P128S (CCA→TCT)	U712_10990 ←	Anthranilate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,193,379	0	T	A	98.3%	183.2 / NA	60	P128S (CCA→TCT)	U712_10990	Anthranilate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (26/33);  total (26/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTCTGAAACCCAGTTCTTTTCTTGTACCCGCTACATGTTTCATAGACGAATGATAGAGCGGTGCAAAAAGAAATCCCATGTTGTTTGCTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATTGGAACCTCTAACACCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCGTGCTTAGCGATTTTTGCCCCTGCTGCTGAGGCAACAATTGCTGATGCCGTTGAGATATTAAAAGTA  >  CP006881/2193251‑2193508                                                                                                                                 |                                                                                                                                  gTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACctc                                                                                                                           >  1:283061/1‑137 (MQ=255) gTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACctc                                                                                                                           >  4:443990/1‑137 (MQ=255) gTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACctc                                                                                                                           >  4:362184/1‑137 (MQ=255) gTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACctc                                                                                                                           >  8:22686/1‑137 (MQ=255) gTTCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACctc                                                                                                                           >  5:361132/1‑137 (MQ=255)  ttCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTag                                                                                                                        <  8:137224/139‑2 (MQ=255)  ttCTGAAACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTag                                                                                                                        <  7:342795/139‑2 (MQ=255)    cTGAAACCTATCTCTTTTCTAGTACCTGCTACATGATTCAGAGACTAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTTCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTagc                                                                                                                       <  5:186090/138‑3 (MQ=255)     tGACACCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGGTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTagc                                                                                                                       <  8:167798/137‑3 (MQ=255)       caaCCTAGCTCTTTTCTAGTACCTGCTACATGTTTCATAGAGGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTagctcc                                                                                                                    <  1:131064/137‑6 (MQ=255)          cctagctcttttctaGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTa                                                                                                                <  7:323705/133‑1 (MQ=255)            tagctcttttctaGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTaa                                                                                                               >  7:94185/5‑138 (MQ=255)            tagctcttttctaGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTaa                                                                                                               >  8:132836/5‑138 (MQ=255)             agctcttttctaGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTaaaa                                                                                                             >  6:396861/4‑139 (MQ=255)                  ttttctaGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACAt                                                                                                          <  5:20787/137‑1 (MQ=255)                           aCCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTcc                                                                                                <  2:283061/138‑1 (MQ=255)                                cTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCttt                                                                                           <  7:66000/138‑1 (MQ=255)                                cTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCtt                                                                                            <  3:362184/137‑1 (MQ=255)                                        ttCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTaa                                                                                                               <  7:239379/110‑1 (MQ=255)                                        ttCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTaa                                                                                                               >  8:239379/1‑110 (MQ=255)                                        ttCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAgaga                                                                                  >  6:220136/1‑139 (MQ=255)                                        ttCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAgaga                                                                                  >  5:339306/1‑139 (MQ=255)                                          cATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAgaga                                                                                  >  7:185362/1‑137 (MQ=255)                                          cATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGAc                                                                                 <  7:132836/138‑1 (MQ=255)                                           aTAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGGTTGATTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACag                                                                               <  4:125337/139‑1 (MQ=255)                                              gACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGc                                                                            <  5:396861/139‑1 (MQ=255)                                               aCGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCTGCTTTTAGAAGAGACAGAGc                                                                            >  6:415376/1‑138 (MQ=255)                                                cGAATGGTAAAGCGTCGCAAAAAGAAATCCCATGTGGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGa                                                                          <  2:171647/139‑1 (MQ=255)                                                cGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGa                                                                          <  2:34824/139‑1 (MQ=255)                                                   aTGGTAAAGCGGCGCTAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTg                                                                       <  4:337454/139‑1 (MQ=255)                                                      gtaaagCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGcc                                                                     <  8:94185/134‑1 (MQ=255)                                                      gtaaagCGGCGCAAAAAGAAATCCCATGTTGTGTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGcc                                                                     <  4:164298/134‑1 (MQ=255)                                                         aagCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGc                                                                 <  3:443990/138‑1 (MQ=255)                                                         aagCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGc                                                                 <  7:22686/138‑1 (MQ=255)                                                               cgCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCtt                                                                                            <  6:79147/105‑1 (MQ=255)                                                               cgCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCtt                                                                                            >  5:79147/2‑106 (MQ=255)                                                               cgCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGc                                                            >  4:27346/2‑138 (MQ=255)                                                               cgCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGc                                                            >  1:53051/2‑138 (MQ=255)                                                                gCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGa                                                          >  5:219664/1‑139 (MQ=255)                                                                                  ttgtttgtTTCAATGCTGCTTTTGACCTATTCGGGAGTGGGTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACcagcagcc                                         <  5:106253/138‑2 (MQ=255)                                                                                     tttGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAgg                                     >  3:390336/1‑139 (MQ=255)                                                                                       tgtttCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGc                                    <  1:27404/135‑1 (MQ=255)                                                                                       tgtttCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGc                                    >  5:102789/4‑138 (MQ=255)                                                                                       tgtttCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGc                                    >  8:337301/4‑138 (MQ=255)                                                                                       tgtttCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGc                                    >  3:282201/4‑138 (MQ=255)                                                                                            cAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGcaacaa                               <  3:27346/138‑1 (MQ=255)                                                                                                 ctgctTTTGACCTTTTCGGGAGTGGTTTGAATTGAAACCTCTAGCTCCTCTAAAACATCCGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGccg                         <  8:185362/139‑3 (MQ=255)                                                                                                 ctgctTTTGACCTTTTCGGGAGTGGGTTGAATAGAAACCTCTAGCTCCTCAAAAACATCAGCGCTTCCGCTTTTAGAAGAGACGGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGccg                         <  3:43144/139‑3 (MQ=255)                                                                                                    ctTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTGCTCTAAAACATCACCGCTTCCGCGTTTATAAGAGCCAGAGCGATTGCCCTGCGTAGCGCTTTTAGCACCAGCAGCCGAGGCACCAATTGCCGagg                      >  2:217426/1‑137 (MQ=255)                                                                                                    ctTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGagg                      <  2:251356/139‑3 (MQ=255)                                                                                                              tttCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTga               >  3:235770/1‑136 (MQ=255)                                                                                                              tttCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTga               <  4:235770/136‑1 (MQ=255)                                                                                                                    gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaaa      <  2:53051/139‑1 (MQ=255)                                                                                                                    gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaaa      <  2:146003/139‑1 (MQ=255)                                                                                                                    gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaaa      <  2:122548/139‑1 (MQ=255)                                                                                                                    gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaaa      <  4:29243/139‑1 (MQ=255)                                                                                                                    gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaaa      <  6:408425/139‑1 (MQ=255)                                                                                                                    gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaa       >  3:29243/1‑138 (MQ=255)                                                                                                                       tGGTTTGAATAGAAACCTCTAGCTCCTCTTAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGGTGAGATATTAAAAGt   >  2:135101/1‑139 (MQ=255)                                                                                                                         gTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGtg  >  5:442731/1‑137 (MQ=255)                                                                                                                                 |                                                                                                                                  GTTCTGAAACCCAGTTCTTTTCTTGTACCCGCTACATGTTTCATAGACGAATGATAGAGCGGTGCAAAAAGAAATCCCATGTTGTTTGCTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATTGGAACCTCTAACACCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCGTGCTTAGCGATTTTTGCCCCTGCTGCTGAGGCAACAATTGCTGATGCCGTTGAGATATTAAAAGTA  >  CP006881/2193251‑2193508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑