Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,193,392	A→T	V124E (GTG→GAG)	U712_10990 ←	Anthranilate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,193,392	0	A	T	100.0%	176.4 / NA	59	V124E (GTG→GAG)	U712_10990	Anthranilate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (25/34);  total (25/34)

CCCAGTTCTTTTCTTGTACCCGCTACATGTTTCATAGACGAATGATAGAGCGGTGCAAAAAGAAATCCCATGTTGTTTGCTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATTGGAACCTCTAACACCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCGTGCTTAGCGATTTTTGCCCCTGCTGCTGAGGCAACAATTGCTGATGCCGTTGAGATATTAAAAGTAGAAATACCGTCTCCTC  >  CP006881/2193260‑2193524                                                                                                                                     |                                                                                                                                     cctagctcttttctaGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTa                                                                                                                                <  7:323705/133‑1 (MQ=255)   tagctcttttctaGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTaa                                                                                                                               >  8:132836/5‑138 (MQ=255)   tagctcttttctaGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTaa                                                                                                                               >  7:94185/5‑138 (MQ=255)    agctcttttctaGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTaaaa                                                                                                                             >  6:396861/4‑139 (MQ=255)         ttttctaGTACCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACAt                                                                                                                          <  5:20787/137‑1 (MQ=255)                  aCCTGCTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTcc                                                                                                                <  2:283061/138‑1 (MQ=255)                       cTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCttt                                                                                                           <  7:66000/138‑1 (MQ=255)                       cTACATGTTTCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCtt                                                                                                            <  3:362184/137‑1 (MQ=255)                               ttCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTaa                                                                                                                               >  8:239379/1‑110 (MQ=255)                               ttCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTaa                                                                                                                               <  7:239379/110‑1 (MQ=255)                               ttCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAgaga                                                                                                  >  5:339306/1‑139 (MQ=255)                               ttCATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAgaga                                                                                                  >  6:220136/1‑139 (MQ=255)                                 cATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAgaga                                                                                                  >  7:185362/1‑137 (MQ=255)                                 cATAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGAc                                                                                                 <  7:132836/138‑1 (MQ=255)                                  aTAGACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGGTTGATTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACag                                                                                               <  4:125337/139‑1 (MQ=255)                                     gACGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGc                                                                                            <  5:396861/139‑1 (MQ=255)                                      aCGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCTGCTTTTAGAAGAGACAGAGc                                                                                            >  6:415376/1‑138 (MQ=255)                                       cGAATGGTAAAGCGTCGCAAAAAGAAATCCCATGTGGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGa                                                                                          <  2:171647/139‑1 (MQ=255)                                       cGAATGGTAAAGCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGa                                                                                          <  2:34824/139‑1 (MQ=255)                                          aTGGTAAAGCGGCGCTAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTg                                                                                       <  4:337454/139‑1 (MQ=255)                                             gtaaagCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGcc                                                                                     <  8:94185/134‑1 (MQ=255)                                             gtaaagCGGCGCAAAAAGAAATCCCATGTTGTGTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGcc                                                                                     <  4:164298/134‑1 (MQ=255)                                                aagCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGc                                                                                 <  7:22686/138‑1 (MQ=255)                                                aagCGGCGCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGc                                                                                 <  3:443990/138‑1 (MQ=255)                                                      cgCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCtt                                                                                                            <  6:79147/105‑1 (MQ=255)                                                      cgCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCtt                                                                                                            >  5:79147/2‑106 (MQ=255)                                                      cgCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGc                                                                            >  4:27346/2‑138 (MQ=255)                                                      cgCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGc                                                                            >  1:53051/2‑138 (MQ=255)                                                       gCAAAAAGAAATCCCATGTTGTTTGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGa                                                                          >  5:219664/1‑139 (MQ=255)                                                                         ttgtttgtTTCAATGCTGCTTTTGACCTATTCGGGAGTGGGTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACcagcagcc                                                         <  5:106253/138‑2 (MQ=255)                                                                            tttGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAgg                                                     >  3:390336/1‑139 (MQ=255)                                                                              tgtttCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGc                                                    >  5:102789/4‑138 (MQ=255)                                                                              tgtttCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGc                                                    >  8:337301/4‑138 (MQ=255)                                                                              tgtttCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGc                                                    <  1:27404/135‑1 (MQ=255)                                                                              tgtttCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGc                                                    >  3:282201/4‑138 (MQ=255)                                                                                   cAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGcaacaa                                               <  3:27346/138‑1 (MQ=255)                                                                                        ctgctTTTGACCTTTTCGGGAGTGGTTTGAATTGAAACCTCTAGCTCCTCTAAAACATCCGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGccg                                         <  8:185362/139‑3 (MQ=255)                                                                                        ctgctTTTGACCTTTTCGGGAGTGGGTTGAATAGAAACCTCTAGCTCCTCAAAAACATCAGCGCTTCCGCTTTTAGAAGAGACGGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGccg                                         <  3:43144/139‑3 (MQ=255)                                                                                           ctTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTGCTCTAAAACATCACCGCTTCCGCGTTTATAAGAGCCAGAGCGATTGCCCTGCGTAGCGCTTTTAGCACCAGCAGCCGAGGCACCAATTGCCGagg                                      >  2:217426/1‑137 (MQ=255)                                                                                           ctTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGagg                                      <  2:251356/139‑3 (MQ=255)                                                                                                     tttCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTga                               <  4:235770/136‑1 (MQ=255)                                                                                                     tttCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTga                               >  3:235770/1‑136 (MQ=255)                                                                                                           gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaaa                      <  4:29243/139‑1 (MQ=255)                                                                                                           gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaaa                      <  2:122548/139‑1 (MQ=255)                                                                                                           gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaaa                      <  2:146003/139‑1 (MQ=255)                                                                                                           gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaaa                      <  2:53051/139‑1 (MQ=255)                                                                                                           gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaaa                      <  6:408425/139‑1 (MQ=255)                                                                                                           gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaa                       >  3:29243/1‑138 (MQ=255)                                                                                                              tGGTTTGAATAGAAACCTCTAGCTCCTCTTAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGGTGAGATATTAAAAGt                   >  2:135101/1‑139 (MQ=255)                                                                                                                gTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGtg                  >  5:442731/1‑137 (MQ=255)                                                                                                                     aatagaaaCCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATa            <  6:339306/133‑1 (MQ=255)                                                                                                                      atagaaaCCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATa            <  6:442731/133‑1 (MQ=255)                                                                                                                          aaaCCTCTAGCTCCTCTAAAACATCAGCGCTTCTGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGtc       <  5:415376/138‑1 (MQ=255)                                                                                                                          aaaCCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGtc       <  2:134956/138‑1 (MQ=255)                                                                                                                          aaaCCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGtc       >  1:134956/2‑139 (MQ=255)                                                                                                                          aaaCCTCAATCTCCTCTAAAACATCAGCGCATCCGCTTTTAGAAGAGACAGAGCGATTGCCATCCTTACCCATTTTCGCACCAGATGCCGACGCAACAATTGCCTAGGCCGTTGAGATATTAAAAGTGGAAATaactt        >  2:10747/2‑134 (MQ=255)                                                                                                                            aCCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGcaccag                                                              >  7:160460/1‑80 (MQ=255)                                                                                                                            aCCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGcaccag                                                              <  8:160460/82‑3 (MQ=255)                                                                                                                               tctAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTctcccc  >  4:258018/1‑137 (MQ=255)                                                                                                                                     |                                                                                                                                     CCCAGTTCTTTTCTTGTACCCGCTACATGTTTCATAGACGAATGATAGAGCGGTGCAAAAAGAAATCCCATGTTGTTTGCTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATTGGAACCTCTAACACCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCGTGCTTAGCGATTTTTGCCCCTGCTGCTGAGGCAACAATTGCTGATGCCGTTGAGATATTAAAAGTAGAAATACCGTCTCCTC  >  CP006881/2193260‑2193524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑