| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 2,173,697 | T→G | G17G (GGA→GGC) | U712_10870 ← | Bifunctional protein BirA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,173,697 | 0 | T | G | 98.4% | 198.9 / ‑4.5 | 62 | G17G (GGA→GGC) | U712_10870 | Bifunctional protein BirA |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (30/31); total (30/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
CCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAGCCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTGGAACAGCCGAGGGCATCACTGATTTTTTGGCCGGAAATAAATTCACTTCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCACTCCTCTATGTGTTTCTTTTGATTGGAGAGCTTTCCTGTCACAACAGCCTGCTCGATCC > CP006881/2173568‑2173817 | ccGATATCCTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCt > 8:227906/1‑137 (MQ=255) cTTTTCTTCTAACGGCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAaataa < 8:285150/139‑1 (MQ=255) aCGGCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGt > 1:319228/1‑139 (MQ=255) ggCTTCTACTTCATAGCCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTGGAACAGCCGAGGGCATCACTGATTTTTTGGCCGGAAATAAATTCACTTCCGGCCTGAGAAAATAATTCAATAAGGTCt < 3:382274/139‑1 (MQ=255) ggCTTCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCt < 5:390276/139‑1 (MQ=255) ttCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAaataa < 7:80501/123‑1 (MQ=255) ttCTACTTCATAACCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAaataa > 8:80501/1‑123 (MQ=255) acccTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGAc < 7:206355/138‑1 (MQ=255) accatctTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGTCCTGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGAc < 8:126247/135‑1 (MQ=255) tttCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCa < 7:3426/137‑1 (MQ=255) tttCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCa < 4:91896/137‑1 (MQ=255) tttCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCa < 2:319228/137‑1 (MQ=255) tttCCGAAGCTCTTCAATATGCTTACACACAGCAGTTATTGAACAGCAGAGAGCTTCACTGATTTTTTGGCAGGAAATAAATTAATTGCCGTCCTGAGAAAATAATTCAATAAGGTCTTTTATTAATGTTGAACGCATg > 8:406845/1‑139 (MQ=255) tCCGAAGCTATTCAATATGCTTCCACACATCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGTAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCa < 7:406845/135‑1 (MQ=255) gAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTc < 4:128616/139‑1 (MQ=255) gAAGCTCTTCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTc < 7:227906/139‑1 (MQ=255) ttCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCa < 6:11534/137‑1 (MQ=255) ttCAATATGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCa < 1:222276/137‑1 (MQ=255) atGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCACTACTCTa < 4:79947/139‑1 (MQ=255) tGCTTCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCACTCCTCTAt > 2:376655/1‑139 (MQ=255) cTTCCACCCAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCACTCCTCTAtgt > 6:277644/1‑139 (MQ=255) ttCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTga < 6:319742/75‑1 (MQ=255) ttCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTga > 5:319742/1‑75 (MQ=255) ttCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCt > 5:398894/1‑96 (MQ=255) ttCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCt < 6:392448/96‑1 (MQ=255) ttCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCt > 5:392448/1‑96 (MQ=255) ttCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCt < 6:398894/96‑1 (MQ=255) ttCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGcc < 4:369216/126‑1 (MQ=255) ttCCACACAGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGcc > 3:369216/1‑126 (MQ=255) cacacaGCAGTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCACTCCTCTATGTGttt > 2:86079/1‑139 (MQ=255) agcagTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATg < 4:29076/98‑1 (MQ=255) agcagTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATg > 3:29076/1‑98 (MQ=255) cagTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTc > 5:179963/1‑90 (MQ=255) cagTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTc < 6:179963/90‑1 (MQ=255) cagTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCACTCCTCTATGTGTTTCTTTTGa > 1:115258/1‑139 (MQ=255) cagTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCACTCCTCTATGTGTTTCTTTTGa > 6:226415/1‑139 (MQ=255) cagTTCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCACTCCTCTATGTGTTTCTTTTGa > 4:386832/1‑139 (MQ=255) ttCTTGAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCACTCCTCTATGTGTTTCTTTTGATTg > 2:429698/1‑139 (MQ=255) cttgAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCTCATGTCTTCAGCCACTCCTCTATGTGTTTCTGTTGATTg > 7:395223/4‑137 (MQ=255) cttgAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCACTCCTCTATGTGTTTCTTTTGATTg > 3:259549/4‑137 (MQ=255) gAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGcc > 3:84382/1‑108 (MQ=255) gAACAGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGcc < 4:84382/108‑1 (MQ=255) aGCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCACTCCTCTATGTGTTTCTTTTGATTGGAGAGCttc < 5:277644/139‑2 (MQ=255) gCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCa < 4:164186/92‑1 (MQ=255) gCCGAGAGCATCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCa > 3:164186/1‑92 (MQ=255) ccGAGAGCAGCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAATGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCACTCCTCTATGTGTTTCTTTTGATTTGAGAGCTTccc > 5:365691/1‑139 (MQ=255) aTCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGAc > 2:96716/1‑79 (MQ=255) aTCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGAc < 1:96716/79‑1 (MQ=255) aTCACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCACTCCTCTATGTGTTTCTTTTGATTGGAGAGCTTCCCTGTCacaa > 2:20693/1‑139 (MQ=255) cACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCa < 1:128242/81‑1 (MQ=255) cACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCa > 2:128242/1‑81 (MQ=255) cACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGcc > 6:182513/1‑92 (MQ=255) cACTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGcc < 5:182513/92‑1 (MQ=255) aCTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCACTCCTCTATGTGTTTCTTTTGATTGGAGAGCTTCCCTGTCacaaca < 7:442120/138‑1 (MQ=255) cTGATTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCACTCCTCTATGTGTTTCTTTTGATTGGAGAGCTTCCCTGTCACAACAGc < 3:386832/139‑1 (MQ=255) aTTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCa > 7:378990/1‑88 (MQ=255) aTTTTTTGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCa < 8:378990/88‑1 (MQ=255) tttttGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCACTCCTCTATGTGTTTCTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCt > 7:110915/1‑139 (MQ=255) ttttGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGc < 4:262208/83‑1 (MQ=255) ttttGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGc > 3:262208/1‑83 (MQ=255) ttttGGCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCACTCCTCTATGTGTTTCTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCt < 8:68288/138‑1 (MQ=255) gCCGGAAATAAATTCATTGCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCACTCCTCTATGTGTTTCTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATcc > 3:66477/1‑139 (MQ=255) | CCGATATCCTTTTCTTCTAACGGCTTCTACTTCATAGCCCTCTTTCCGAAGCTCTTCAATATGCTTCCACACAGCAGTTCTGGAACAGCCGAGGGCATCACTGATTTTTTGGCCGGAAATAAATTCACTTCCGGCCTGAGAAAATAATTCAATAAGGTCTTTTCTTAATGTTGACCGCATGTCTTCAGCCACTCCTCTATGTGTTTCTTTTGATTGGAGAGCTTTCCTGTCACAACAGCCTGCTCGATCC > CP006881/2173568‑2173817 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |