Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,193,457 T→C A102A (GCA→GCG)  U712_10990 ← Anthranilate phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,193,4570TC98.3% 186.4 / NA 59A102A (GCA→GCG) U712_10990Anthranilate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (25/33);  minor base A (1/0);  total (26/33)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGTTTGCTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATTGGAACCTCTAACACCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCGTGCTTAGCGATTTTTGCCCCTGCTGCTGAGGCAACAATTGCTGATGCCGTTGAGATATTAAAAGTAGAAATACCGTCTCCTCCGGTTCCGCATGTATCAACGACATCAGGAAGTCCATCGACTGTAAGAGCGCGTGCCCGCATCGCC  >  CP006881/2193332‑2193589
                                                                                                                             |                                                                                                                                    
ttgtttgtTTCAATGCTGCTTTTGACCTATTCGGGAGTGGGTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACcagcagcc                                                                                                                          <  5:106253/138‑2 (MQ=255)
   tttGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAgg                                                                                                                      >  3:390336/1‑139 (MQ=255)
     tgtttCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGc                                                                                                                     <  1:27404/135‑1 (MQ=255)
     tgtttCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGc                                                                                                                     >  8:337301/4‑138 (MQ=255)
     tgtttCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGc                                                                                                                     >  3:282201/4‑138 (MQ=255)
     tgtttCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGc                                                                                                                     >  5:102789/4‑138 (MQ=255)
          cAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGcaacaa                                                                                                                <  3:27346/138‑1 (MQ=255)
               ctgctTTTGACCTTTTCGGGAGTGGTTTGAATTGAAACCTCTAGCTCCTCTAAAACATCCGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGccg                                                                                                          <  8:185362/139‑3 (MQ=255)
               ctgctTTTGACCTTTTCGGGAGTGGGTTGAATAGAAACCTCTAGCTCCTCAAAAACATCAGCGCTTCCGCTTTTAGAAGAGACGGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGccg                                                                                                          <  3:43144/139‑3 (MQ=255)
                  ctTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTGCTCTAAAACATCACCGCTTCCGCGTTTATAAGAGCCAGAGCGATTGCCCTGCGTAGCGCTTTTAGCACCAGCAGCCGAGGCACCAATTGCCGagg                                                                                                       >  2:217426/1‑137 (MQ=255)
                  ctTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGagg                                                                                                       <  2:251356/139‑3 (MQ=255)
                            tttCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTga                                                                                                >  3:235770/1‑136 (MQ=255)
                            tttCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTga                                                                                                <  4:235770/136‑1 (MQ=255)
                                  gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaaa                                                                                       <  2:146003/139‑1 (MQ=255)
                                  gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaaa                                                                                       <  2:53051/139‑1 (MQ=255)
                                  gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaaa                                                                                       <  6:408425/139‑1 (MQ=255)
                                  gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaaa                                                                                       <  4:29243/139‑1 (MQ=255)
                                  gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaaa                                                                                       <  2:122548/139‑1 (MQ=255)
                                  gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaa                                                                                        >  3:29243/1‑138 (MQ=255)
                                     tGGTTTGAATAGAAACCTCTAGCTCCTCTTAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGGTGAGATATTAAAAGt                                                                                    >  2:135101/1‑139 (MQ=255)
                                       gTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGtg                                                                                   >  5:442731/1‑137 (MQ=255)
                                            aatagaaaCCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATa                                                                             <  6:339306/133‑1 (MQ=255)
                                             atagaaaCCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATa                                                                             <  6:442731/133‑1 (MQ=255)
                                                 aaaCCTCTAGCTCCTCTAAAACATCAGCGCTTCTGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGtc                                                                        <  5:415376/138‑1 (MQ=255)
                                                 aaaCCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGtc                                                                        >  1:134956/2‑139 (MQ=255)
                                                 aaaCCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGtc                                                                        <  2:134956/138‑1 (MQ=255)
                                                 aaaCCTCAATCTCCTCTAAAACATCAGCGCATCCGCTTTTAGAAGAGACAGAGCGATTGCCATCCTTACCCATTTTCGCACCAGATGCCGACGCAACAATTGCCTAGGCCGTTGAGATATTAAAAGTGGAAATaactt                                                                         >  2:10747/2‑134 (MQ=255)
                                                   aCCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGcaccag                                                                                                                               >  7:160460/1‑80 (MQ=255)
                                                   aCCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGcaccag                                                                                                                               <  8:160460/82‑3 (MQ=255)
                                                      tctAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTctcccc                                                                   >  4:258018/1‑137 (MQ=255)
                                                           ctccTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAg                                                                                     <  5:119655/114‑1 (MQ=255)
                                                           ctccTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAg                                                                                     >  6:119655/3‑116 (MQ=255)
                                                              ctctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCg                                                           <  3:210150/139‑1 (MQ=255)
                                                              ctctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTCAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCg                                                           >  2:413448/1‑139 (MQ=255)
                                                               tctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCg                                                           >  3:305632/1‑138 (MQ=255)
                                                               tctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCg                                                           >  5:186445/1‑138 (MQ=255)
                                                               tctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCg                                                           >  5:278762/1‑138 (MQ=255)
                                                               tctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCg                                                           >  8:424551/1‑138 (MQ=255)
                                                               tctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGc                                                          >  4:6842/1‑139 (MQ=255)
                                                                ctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCa                                                         >  8:11734/1‑139 (MQ=255)
                                                                ctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTGCCGCa                                                         >  8:5200/1‑139 (MQ=255)
                                                                      cATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATc                                                   <  2:195402/139‑1 (MQ=255)
                                                                        tCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACACTTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCaa                                                 >  6:235489/1‑139 (MQ=255)
                                                                        tCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCaa                                                 >  1:50244/1‑139 (MQ=255)
                                                                                   gCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGa                                      <  6:102789/139‑1 (MQ=255)
                                                                                   gCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGa                                      <  7:424551/139‑1 (MQ=255)
                                                                                           aagaGCCAGAGCGATTGCCATGCTTAGCTTTTTTCGCACCAGCTGCCGAGGCAACAATTGCCGTGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATc                              <  1:217426/139‑1 (MQ=255)
                                                                                           aagaGACCGAGCGATTGCCAGGCTTAGGTATTTTCGCACCCGCGGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATCCCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATc                              <  4:390336/139‑1 (MQ=255)
                                                                                           aagaGACCGAGAGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAAAACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATc                              <  5:220136/139‑1 (MQ=255)
                                                                                           aagaGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATc                              <  6:186445/139‑1 (MQ=255)
                                                                                           aagaGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATc                              <  5:235489/139‑1 (MQ=255)
                                                                                           aagaGACAGAGCGATTGCCATGCTCAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATc                              <  1:413448/139‑1 (MQ=255)
                                                                                             gagaCAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATCGa                            <  7:337301/139‑1 (MQ=255)
                                                                                             gagaCAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATCGa                            <  8:134023/139‑1 (MQ=255)
                                                                                                         ttGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGtt                                                              <  5:225799/93‑1 (MQ=255)
                                                                                                         ttGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGtt                                                              >  6:225799/1‑93 (MQ=255)
                                                                                                                    aGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATCGACTGTAAGAGCGTGTGCCCGCAtt     >  2:53570/1‑138 (MQ=255)
                                                                                                                      cGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATCGACTGTAAGAGCGTGTGCCCGCATTGc   <  1:53570/139‑1 (MQ=255)
                                                                                                                       gATTTTCCCACCAGCTGCCGCGGAAACAATTGCCGAGGCCGTTTAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGGCCATCGACTGTAAGAGCGTGTGCCCGCATTGcc  <  6:278762/139‑1 (MQ=255)
                                                                                                                             |                                                                                                                                    
TTGTTTGCTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATTGGAACCTCTAACACCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCGTGCTTAGCGATTTTTGCCCCTGCTGCTGAGGCAACAATTGCTGATGCCGTTGAGATATTAAAAGTAGAAATACCGTCTCCTCCGGTTCCGCATGTATCAACGACATCAGGAAGTCCATCGACTGTAAGAGCGCGTGCCCGCATCGCC  >  CP006881/2193332‑2193589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑