Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,193,469	T→C	S98S (TCA→TCG)	U712_10990 ←	Anthranilate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,193,469	0	T	C	100.0%	180.2 / NA	56	S98S (TCA→TCG)	U712_10990	Anthranilate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/31);  total (25/31)

TTTGCTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATTGGAACCTCTAACACCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCGTGCTTAGCGATTTTTGCCCCTGCTGCTGAGGCAACAATTGCTGATGCCGTTGAGATATTAAAAGTAGAAATACCGTCTCCTCCGGTTCCGCATGTATCAACGACATCAGGAAGTCCATCGACTGTAAGAGCGCGTGCCCGCATCGCC  >  CP006881/2193335‑2193589                                                                                                                                       |                                                                                                                         tttGTTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAgg                                                                                                                      >  3:390336/1‑139 (MQ=255)   tgtttCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGc                                                                                                                     >  5:102789/4‑138 (MQ=255)   tgtttCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGc                                                                                                                     >  8:337301/4‑138 (MQ=255)   tgtttCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGc                                                                                                                     >  3:282201/4‑138 (MQ=255)   tgtttCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGc                                                                                                                     <  1:27404/135‑1 (MQ=255)        cAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGcaacaa                                                                                                                <  3:27346/138‑1 (MQ=255)             ctgctTTTGACCTTTTCGGGAGTGGTTTGAATTGAAACCTCTAGCTCCTCTAAAACATCCGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGccg                                                                                                          <  8:185362/139‑3 (MQ=255)             ctgctTTTGACCTTTTCGGGAGTGGGTTGAATAGAAACCTCTAGCTCCTCAAAAACATCAGCGCTTCCGCTTTTAGAAGAGACGGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGccg                                                                                                          <  3:43144/139‑3 (MQ=255)                ctTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTGCTCTAAAACATCACCGCTTCCGCGTTTATAAGAGCCAGAGCGATTGCCCTGCGTAGCGCTTTTAGCACCAGCAGCCGAGGCACCAATTGCCGagg                                                                                                       >  2:217426/1‑137 (MQ=255)                ctTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGagg                                                                                                       <  2:251356/139‑3 (MQ=255)                          tttCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTga                                                                                                <  4:235770/136‑1 (MQ=255)                          tttCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTga                                                                                                >  3:235770/1‑136 (MQ=255)                                gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaaa                                                                                       <  6:408425/139‑1 (MQ=255)                                gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaaa                                                                                       <  4:29243/139‑1 (MQ=255)                                gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaaa                                                                                       <  2:122548/139‑1 (MQ=255)                                gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaaa                                                                                       <  2:146003/139‑1 (MQ=255)                                gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaaa                                                                                       <  2:53051/139‑1 (MQ=255)                                gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaa                                                                                        >  3:29243/1‑138 (MQ=255)                                   tGGTTTGAATAGAAACCTCTAGCTCCTCTTAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGGTGAGATATTAAAAGt                                                                                    >  2:135101/1‑139 (MQ=255)                                     gTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGtg                                                                                   >  5:442731/1‑137 (MQ=255)                                          aatagaaaCCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATa                                                                             <  6:339306/133‑1 (MQ=255)                                           atagaaaCCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATa                                                                             <  6:442731/133‑1 (MQ=255)                                               aaaCCTCTAGCTCCTCTAAAACATCAGCGCTTCTGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGtc                                                                        <  5:415376/138‑1 (MQ=255)                                               aaaCCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGtc                                                                        <  2:134956/138‑1 (MQ=255)                                               aaaCCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGtc                                                                        >  1:134956/2‑139 (MQ=255)                                               aaaCCTCAATCTCCTCTAAAACATCAGCGCATCCGCTTTTAGAAGAGACAGAGCGATTGCCATCCTTACCCATTTTCGCACCAGATGCCGACGCAACAATTGCCTAGGCCGTTGAGATATTAAAAGTGGAAATaactt                                                                         >  2:10747/2‑134 (MQ=255)                                                    tctAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTctcccc                                                                   >  4:258018/1‑137 (MQ=255)                                                         ctccTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAg                                                                                     >  6:119655/3‑116 (MQ=255)                                                         ctccTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAg                                                                                     <  5:119655/114‑1 (MQ=255)                                                            ctctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCg                                                           <  3:210150/139‑1 (MQ=255)                                                            ctctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTCAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCg                                                           >  2:413448/1‑139 (MQ=255)                                                             tctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCg                                                           >  5:278762/1‑138 (MQ=255)                                                             tctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCg                                                           >  3:305632/1‑138 (MQ=255)                                                             tctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCg                                                           >  5:186445/1‑138 (MQ=255)                                                             tctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCg                                                           >  8:424551/1‑138 (MQ=255)                                                             tctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGc                                                          >  4:6842/1‑139 (MQ=255)                                                              ctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCa                                                         >  8:11734/1‑139 (MQ=255)                                                              ctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTGCCGCa                                                         >  8:5200/1‑139 (MQ=255)                                                                    cATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATc                                                   <  2:195402/139‑1 (MQ=255)                                                                      tCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACACTTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCaa                                                 >  6:235489/1‑139 (MQ=255)                                                                      tCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCaa                                                 >  1:50244/1‑139 (MQ=255)                                                                                 gCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGa                                      <  7:424551/139‑1 (MQ=255)                                                                                 gCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGa                                      <  6:102789/139‑1 (MQ=255)                                                                                         aagaGCCAGAGCGATTGCCATGCTTAGCTTTTTTCGCACCAGCTGCCGAGGCAACAATTGCCGTGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATc                              <  1:217426/139‑1 (MQ=255)                                                                                         aagaGACCGAGCGATTGCCAGGCTTAGGTATTTTCGCACCCGCGGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATCCCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATc                              <  4:390336/139‑1 (MQ=255)                                                                                         aagaGACCGAGAGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAAAACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATc                              <  5:220136/139‑1 (MQ=255)                                                                                         aagaGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATc                              <  6:186445/139‑1 (MQ=255)                                                                                         aagaGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATc                              <  5:235489/139‑1 (MQ=255)                                                                                         aagaGACAGAGCGATTGCCATGCTCAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATc                              <  1:413448/139‑1 (MQ=255)                                                                                           gagaCAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATCGa                            <  7:337301/139‑1 (MQ=255)                                                                                           gagaCAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATCGa                            <  8:134023/139‑1 (MQ=255)                                                                                                       ttGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGtt                                                              >  6:225799/1‑93 (MQ=255)                                                                                                       ttGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGtt                                                              <  5:225799/93‑1 (MQ=255)                                                                                                                  aGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATCGACTGTAAGAGCGTGTGCCCGCAtt     >  2:53570/1‑138 (MQ=255)                                                                                                                    cGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATCGACTGTAAGAGCGTGTGCCCGCATTGc   <  1:53570/139‑1 (MQ=255)                                                                                                                     gATTTTCCCACCAGCTGCCGCGGAAACAATTGCCGAGGCCGTTTAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGGCCATCGACTGTAAGAGCGTGTGCCCGCATTGcc  <  6:278762/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                         TTTGCTTCAATGCTGCTTTTGACCTTTTCGGGAGTGGTTTGAATTGGAACCTCTAACACCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCGTGCTTAGCGATTTTTGCCCCTGCTGCTGAGGCAACAATTGCTGATGCCGTTGAGATATTAAAAGTAGAAATACCGTCTCCTCCGGTTCCGCATGTATCAACGACATCAGGAAGTCCATCGACTGTAAGAGCGCGTGCCCGCATCGCC  >  CP006881/2193335‑2193589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑