Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,193,484	T→C	S93S (TCA→TCG)	U712_10990 ←	Anthranilate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,193,484	0	T	C	100.0%	165.0 / NA	54	S93S (TCA→TCG)	U712_10990	Anthranilate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/33);  total (21/33)

CTTTTGACCTTTTCGGGAGTGGTTTGAATTGGAACCTCTAACACCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCGTGCTTAGCGATTTTTGCCCCTGCTGCTGAGGCAACAATTGCTGATGCCGTTGAGATATTAAAAGTAGAAATACCGTCTCCTCCGGTTCCGCATGTATCAACGACATCAGGAAGTCCATCGACTGTAAGAGCGCGTGCCCGCATCGCCTTTACAAAGCCCGTTAG  >  CP006881/2193350‑2193606                                                                                                                                       |                                                                                                                           ctTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTGCTCTAAAACATCACCGCTTCCGCGTTTATAAGAGCCAGAGCGATTGCCCTGCGTAGCGCTTTTAGCACCAGCAGCCGAGGCACCAATTGCCGagg                                                                                                                        >  2:217426/1‑137 (MQ=255) ctTTTGACCTTTTCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGagg                                                                                                                        <  2:251356/139‑3 (MQ=255)           tttCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTga                                                                                                                 <  4:235770/136‑1 (MQ=255)           tttCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTga                                                                                                                 >  3:235770/1‑136 (MQ=255)                 gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaaa                                                                                                        <  2:146003/139‑1 (MQ=255)                 gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaaa                                                                                                        <  2:53051/139‑1 (MQ=255)                 gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaaa                                                                                                        <  4:29243/139‑1 (MQ=255)                 gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaaa                                                                                                        <  2:122548/139‑1 (MQ=255)                 gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaaa                                                                                                        <  6:408425/139‑1 (MQ=255)                 gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaa                                                                                                         >  3:29243/1‑138 (MQ=255)                    tGGTTTGAATAGAAACCTCTAGCTCCTCTTAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGGTGAGATATTAAAAGt                                                                                                     >  2:135101/1‑139 (MQ=255)                      gTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGtg                                                                                                    >  5:442731/1‑137 (MQ=255)                           aatagaaaCCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATa                                                                                              <  6:339306/133‑1 (MQ=255)                            atagaaaCCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATa                                                                                              <  6:442731/133‑1 (MQ=255)                                aaaCCTCTAGCTCCTCTAAAACATCAGCGCTTCTGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGtc                                                                                         <  5:415376/138‑1 (MQ=255)                                aaaCCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGtc                                                                                         <  2:134956/138‑1 (MQ=255)                                aaaCCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGtc                                                                                         >  1:134956/2‑139 (MQ=255)                                aaaCCTCAATCTCCTCTAAAACATCAGCGCATCCGCTTTTAGAAGAGACAGAGCGATTGCCATCCTTACCCATTTTCGCACCAGATGCCGACGCAACAATTGCCTAGGCCGTTGAGATATTAAAAGTGGAAATaactt                                                                                          >  2:10747/2‑134 (MQ=255)                                     tctAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTctcccc                                                                                    >  4:258018/1‑137 (MQ=255)                                          ctccTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAg                                                                                                      >  6:119655/3‑116 (MQ=255)                                          ctccTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAg                                                                                                      <  5:119655/114‑1 (MQ=255)                                             ctctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCg                                                                            <  3:210150/139‑1 (MQ=255)                                             ctctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTCAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCg                                                                            >  2:413448/1‑139 (MQ=255)                                              tctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCg                                                                            >  3:305632/1‑138 (MQ=255)                                              tctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCg                                                                            >  8:424551/1‑138 (MQ=255)                                              tctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCg                                                                            >  5:186445/1‑138 (MQ=255)                                              tctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCg                                                                            >  5:278762/1‑138 (MQ=255)                                              tctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGc                                                                           >  4:6842/1‑139 (MQ=255)                                               ctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCa                                                                          >  8:11734/1‑139 (MQ=255)                                               ctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTGCCGCa                                                                          >  8:5200/1‑139 (MQ=255)                                                     cATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATc                                                                    <  2:195402/139‑1 (MQ=255)                                                       tCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACACTTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCaa                                                                  >  6:235489/1‑139 (MQ=255)                                                       tCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCaa                                                                  >  1:50244/1‑139 (MQ=255)                                                                  gCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGa                                                       <  7:424551/139‑1 (MQ=255)                                                                  gCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGa                                                       <  6:102789/139‑1 (MQ=255)                                                                          aagaGCCAGAGCGATTGCCATGCTTAGCTTTTTTCGCACCAGCTGCCGAGGCAACAATTGCCGTGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATc                                               <  1:217426/139‑1 (MQ=255)                                                                          aagaGACCGAGCGATTGCCAGGCTTAGGTATTTTCGCACCCGCGGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATCCCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATc                                               <  4:390336/139‑1 (MQ=255)                                                                          aagaGACCGAGAGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAAAACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATc                                               <  5:220136/139‑1 (MQ=255)                                                                          aagaGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATc                                               <  5:235489/139‑1 (MQ=255)                                                                          aagaGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATc                                               <  6:186445/139‑1 (MQ=255)                                                                          aagaGACAGAGCGATTGCCATGCTCAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATc                                               <  1:413448/139‑1 (MQ=255)                                                                            gagaCAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATCGa                                             <  8:134023/139‑1 (MQ=255)                                                                            gagaCAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATCGa                                             <  7:337301/139‑1 (MQ=255)                                                                                        ttGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGtt                                                                               >  6:225799/1‑93 (MQ=255)                                                                                        ttGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGtt                                                                               <  5:225799/93‑1 (MQ=255)                                                                                                   aGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATCGACTGTAAGAGCGTGTGCCCGCAtt                      >  2:53570/1‑138 (MQ=255)                                                                                                     cGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATCGACTGTAAGAGCGTGTGCCCGCATTGc                    <  1:53570/139‑1 (MQ=255)                                                                                                      gATTTTCCCACCAGCTGCCGCGGAAACAATTGCCGAGGCCGTTTAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGGCCATCGACTGTAAGAGCGTGTGCCCGCATTGcc                   <  6:278762/139‑1 (MQ=255)                                                                                                                       ccgAGGCAACAATTGCCGAGGCCGTTGAGATTTTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATCGACTGTAAGAGCGTGTGCCCGCATTGCCTTCACAAAACCCgcaag  <  1:5743/137‑5 (MQ=255)                                                                                                                       ccgAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCCTGTATCAACAATATCAGGAAGTCCATCGACTGTAAGAGCGTGTGCCCGCATTGCCTTCACAAAACCCgcaag  <  1:143518/137‑5 (MQ=255)                                                                                                                       ccgAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATCGACTGTAAGAGCGTGTGCCCGCATTGCCTTCACAAAACCCgcaag  <  7:11734/137‑5 (MQ=255)                                                                                                                       ccgAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATCGACTGTAAGAGCGTGTGCCCGCATTGCCTTCACAAAACCCgcaag  <  6:445814/137‑5 (MQ=255)                                                                                                                       ccgAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCGCCCACTGTTCCGCATGTATCTACAATATCAGGAAGTCCATCGACTGTAAGAGCGTGTGCCCGCATTGCCTTCACAAAACCCgcaag  <  6:219664/137‑5 (MQ=255)                                                                                                                       ccgAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGGCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATCGACTGTAAGAGCGTGTGCCCGCATTGCCTTCACAAAACCCgcaag  <  7:5200/137‑5 (MQ=255)                                                                                                                                       |                                                                                                                           CTTTTGACCTTTTCGGGAGTGGTTTGAATTGGAACCTCTAACACCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCGTGCTTAGCGATTTTTGCCCCTGCTGCTGAGGCAACAATTGCTGATGCCGTTGAGATATTAAAAGTAGAAATACCGTCTCCTCCGGTTCCGCATGTATCAACGACATCAGGAAGTCCATCGACTGTAAGAGCGCGTGCCCGCATCGCCTTTACAAAGCCCGTTAG  >  CP006881/2193350‑2193606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑