Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,193,487	T→G	A92A (GCA→GCC)	U712_10990 ←	Anthranilate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,193,487	0	T	G	100.0%	166.9 / NA	52	A92A (GCA→GCC)	U712_10990	Anthranilate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (20/32);  total (20/32)

TTTCGGGAGTGGTTTGAATTGGAACCTCTAACACCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCGTGCTTAGCGATTTTTGCCCCTGCTGCTGAGGCAACAATTGCTGATGCCGTTGAGATATTAAAAGTAGAAATACCGTCTCCTCCGGTTCCGCATGTATCAACGACATCAGGAAGTCCATCGACTGTAAGAGCGCGTGCCCGCATCGCCTTTACAAAGCCCGTTAG  >  CP006881/2193360‑2193606                                                                                                                                |                                                                                                                        tttCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTga                                                                                                                 <  4:235770/136‑1 (MQ=255) tttCGGGAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTga                                                                                                                 >  3:235770/1‑136 (MQ=255)       gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaaa                                                                                                        <  4:29243/139‑1 (MQ=255)       gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaaa                                                                                                        <  6:408425/139‑1 (MQ=255)       gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaaa                                                                                                        <  2:122548/139‑1 (MQ=255)       gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaaa                                                                                                        <  2:146003/139‑1 (MQ=255)       gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaaa                                                                                                        <  2:53051/139‑1 (MQ=255)       gAGTGGTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTaa                                                                                                         >  3:29243/1‑138 (MQ=255)          tGGTTTGAATAGAAACCTCTAGCTCCTCTTAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGGTGAGATATTAAAAGt                                                                                                     >  2:135101/1‑139 (MQ=255)            gTTTGAATAGAAACCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGtg                                                                                                    >  5:442731/1‑137 (MQ=255)                 aatagaaaCCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATa                                                                                              <  6:339306/133‑1 (MQ=255)                  atagaaaCCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATa                                                                                              <  6:442731/133‑1 (MQ=255)                      aaaCCTCTAGCTCCTCTAAAACATCAGCGCTTCTGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGtc                                                                                         <  5:415376/138‑1 (MQ=255)                      aaaCCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGtc                                                                                         >  1:134956/2‑139 (MQ=255)                      aaaCCTCTAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGtc                                                                                         <  2:134956/138‑1 (MQ=255)                      aaaCCTCAATCTCCTCTAAAACATCAGCGCATCCGCTTTTAGAAGAGACAGAGCGATTGCCATCCTTACCCATTTTCGCACCAGATGCCGACGCAACAATTGCCTAGGCCGTTGAGATATTAAAAGTGGAAATaactt                                                                                          >  2:10747/2‑134 (MQ=255)                           tctAGCTCCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTctcccc                                                                                    >  4:258018/1‑137 (MQ=255)                                ctccTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAg                                                                                                      >  6:119655/3‑116 (MQ=255)                                ctccTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAg                                                                                                      <  5:119655/114‑1 (MQ=255)                                   ctctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCg                                                                            <  3:210150/139‑1 (MQ=255)                                   ctctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTCAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCg                                                                            >  2:413448/1‑139 (MQ=255)                                    tctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCg                                                                            >  3:305632/1‑138 (MQ=255)                                    tctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCg                                                                            >  8:424551/1‑138 (MQ=255)                                    tctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCg                                                                            >  5:186445/1‑138 (MQ=255)                                    tctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCg                                                                            >  5:278762/1‑138 (MQ=255)                                    tctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGc                                                                           >  4:6842/1‑139 (MQ=255)                                     ctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCa                                                                          >  8:11734/1‑139 (MQ=255)                                     ctAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTGCCGCa                                                                          >  8:5200/1‑139 (MQ=255)                                           cATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATc                                                                    <  2:195402/139‑1 (MQ=255)                                             tCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACACTTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCaa                                                                  >  6:235489/1‑139 (MQ=255)                                             tCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCaa                                                                  >  1:50244/1‑139 (MQ=255)                                                        gCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGa                                                       <  7:424551/139‑1 (MQ=255)                                                        gCTTTTAGAAGAGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGa                                                       <  6:102789/139‑1 (MQ=255)                                                                aagaGCCAGAGCGATTGCCATGCTTAGCTTTTTTCGCACCAGCTGCCGAGGCAACAATTGCCGTGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATc                                               <  1:217426/139‑1 (MQ=255)                                                                aagaGACCGAGCGATTGCCAGGCTTAGGTATTTTCGCACCCGCGGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATCCCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATc                                               <  4:390336/139‑1 (MQ=255)                                                                aagaGACCGAGAGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAAAACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATc                                               <  5:220136/139‑1 (MQ=255)                                                                aagaGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATc                                               <  5:235489/139‑1 (MQ=255)                                                                aagaGACAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATc                                               <  6:186445/139‑1 (MQ=255)                                                                aagaGACAGAGCGATTGCCATGCTCAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATc                                               <  1:413448/139‑1 (MQ=255)                                                                  gagaCAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATCGa                                             <  8:134023/139‑1 (MQ=255)                                                                  gagaCAGAGCGATTGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATCGa                                             <  7:337301/139‑1 (MQ=255)                                                                              ttGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGtt                                                                               >  6:225799/1‑93 (MQ=255)                                                                              ttGCCATGCTTAGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGtt                                                                               <  5:225799/93‑1 (MQ=255)                                                                                         aGCGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATCGACTGTAAGAGCGTGTGCCCGCAtt                      >  2:53570/1‑138 (MQ=255)                                                                                           cGATTTTCGCACCAGCTGCCGAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATCGACTGTAAGAGCGTGTGCCCGCATTGc                    <  1:53570/139‑1 (MQ=255)                                                                                            gATTTTCCCACCAGCTGCCGCGGAAACAATTGCCGAGGCCGTTTAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGGCCATCGACTGTAAGAGCGTGTGCCCGCATTGcc                   <  6:278762/139‑1 (MQ=255)                                                                                                             ccgAGGCAACAATTGCCGAGGCCGTTGAGATTTTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATCGACTGTAAGAGCGTGTGCCCGCATTGCCTTCACAAAACCCgcaag  <  1:5743/137‑5 (MQ=255)                                                                                                             ccgAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCCTGTATCAACAATATCAGGAAGTCCATCGACTGTAAGAGCGTGTGCCCGCATTGCCTTCACAAAACCCgcaag  <  1:143518/137‑5 (MQ=255)                                                                                                             ccgAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATCGACTGTAAGAGCGTGTGCCCGCATTGCCTTCACAAAACCCgcaag  <  7:11734/137‑5 (MQ=255)                                                                                                             ccgAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATCGACTGTAAGAGCGTGTGCCCGCATTGCCTTCACAAAACCCgcaag  <  6:445814/137‑5 (MQ=255)                                                                                                             ccgAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGTCGCCCACTGTTCCGCATGTATCTACAATATCAGGAAGTCCATCGACTGTAAGAGCGTGTGCCCGCATTGCCTTCACAAAACCCgcaag  <  6:219664/137‑5 (MQ=255)                                                                                                             ccgAGGCAACAATTGCCGAGGCCGTTGAGATATTAAAAGTGGAAATACCGGCTCCCCCTGTTCCGCATGTATCAACAATATCAGGAAGTCCATCGACTGTAAGAGCGTGTGCCCGCATTGCCTTCACAAAACCCgcaag  <  7:5200/137‑5 (MQ=255)                                                                                                                                |                                                                                                                        TTTCGGGAGTGGTTTGAATTGGAACCTCTAACACCTCTAAAACATCAGCGCTTCCGCTTTTAGAAGAGACAGAGCGATTGCCGTGCTTAGCGATTTTTGCCCCTGCTGCTGAGGCAACAATTGCTGATGCCGTTGAGATATTAAAAGTAGAAATACCGTCTCCTCCGGTTCCGCATGTATCAACGACATCAGGAAGTCCATCGACTGTAAGAGCGCGTGCCCGCATCGCCTTTACAAAGCCCGTTAG  >  CP006881/2193360‑2193606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑