Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,193,667	T→A	A32T (GCA→ACT)	U712_10990 ←	Anthranilate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,193,667	0	T	A	100.0%	70.7 / NA	24	A32T (GCA→ACT)	U712_10990	Anthranilate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (10/14);  total (10/14)

TGCCCGCATCGCCTTTACAAAGCCCGTTAGCTCTTCTGGCGTCTCTCCCCGATGAGCAAGAATAGACAATATACCGCCCATTTCAGAAGGTGCCATTTCCGCTGTTATCATCATATTCATCAGCGCTTCTGCCTCATCGGCAGTAAGTGTTTTTCCGTCAACGCACAATTGTAGAAATTTGTTCATCAGCTTTATCCTCCTTGCTATGGAACATGTCTTC  >  CP006881/2193577‑2193796                                                                                           |                                                                                                                                  tGCCCGCATTGCCTTCACAAAACCCGCAAGCTCTTCTGGCGTCTCCCCCCGATGAGCAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCacc                                                                                    <  7:417997/138‑3 (MQ=255)                                         gTCTCCCCCCGATGAGCAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAAtc                                           <  5:89383/139‑2 (MQ=255)                                                cccGATGAGCAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTtcat                                    <  5:346485/139‑1 (MQ=255)                                                cccGATGAGCAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTtcat                                    <  5:286302/139‑1 (MQ=255)                                                cccGATGAGCAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATAGTAGAAATCTGTtcat                                    <  7:227400/139‑1 (MQ=255)                                                accGATGAGCAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTtcat                                    <  3:394844/138‑1 (MQ=255)                                                 ccGATGAGCAAGATTTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCGGCCTCCCCGGCAGGAAGGGGTTTTCCGGCACCGCACAAGTGTAGAACTCTGGtcatc                                   >  2:381117/1‑139 (MQ=255)                                                     tGAGCAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCt                               <  3:235779/139‑1 (MQ=255)                                                        gCAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGt                                                                >  8:196583/1‑103 (MQ=255)                                                        gCAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGt                                                                <  7:196583/103‑1 (MQ=255)                                                        gCAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTa                            >  3:300034/1‑139 (MQ=255)                                                         cAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACaa                                                      >  1:99450/1‑112 (MQ=255)                                                         cAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACaa                                                      <  2:99450/112‑1 (MQ=255)                                                         cAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTa                            >  2:429449/1‑138 (MQ=255)                                                              attgACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTAtcctcc                      <  5:145964/136‑1 (MQ=255)                                                                      tatcCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCATCCTTGCTAt               >  4:64485/1‑138 (MQ=255)                                                                          ccccccATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAAc          <  1:272308/136‑1 (MQ=255)                                                                           cccccATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAAc          >  3:82886/3‑138 (MQ=255)                                                                           cccccATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGACTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAAc          <  4:82886/136‑1 (MQ=255)                                                                            ccccATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCacc                                                                                    >  2:150473/2‑61 (MQ=255)                                                                            ccccATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCacc                                                                                    <  1:150473/62‑3 (MQ=255)                                                                             cccATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATg       >  6:332884/1‑139 (MQ=255)                                                                               cATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGtc     <  1:135101/139‑1 (MQ=255)                                                                                  ttCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGtcttc  >  5:119277/1‑139 (MQ=255)                                                                                           |                                                                                                                                  TGCCCGCATCGCCTTTACAAAGCCCGTTAGCTCTTCTGGCGTCTCTCCCCGATGAGCAAGAATAGACAATATACCGCCCATTTCAGAAGGTGCCATTTCCGCTGTTATCATCATATTCATCAGCGCTTCTGCCTCATCGGCAGTAAGTGTTTTTCCGTCAACGCACAATTGTAGAAATTTGTTCATCAGCTTTATCCTCCTTGCTATGGAACATGTCTTC  >  CP006881/2193577‑2193796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑