Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,193,702 C→T A21T (GCG→ACG)  U712_10990 ← Anthranilate phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,193,7020CT100.0% 125.4 / NA 41A21T (GCG→ACG) U712_10990Anthranilate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/21);  total (20/21)

TGCCCGCATCGCCTTTACAAAGCCCGTTAGCTCTTCTGGCGTCTCTCCCCGATGAGCAAGAATAGACAATATACCGCCCATTTCAGAAGGTGCCATTTCCGCTGTTATCATCATATTCATCAGCGCTTCTGCCTCATCGGCAGTAAGTGTTTTTCCGTCAACGCACAATTGTAGAAATTTGTTCATCAGCTTTATCCTCCTTGCTATGGAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTAT  >  CP006881/2193577‑2193834
                                                                                                                             |                                                                                                                                    
tGCCCGCATTGCCTTCACAAAACCCGCAAGCTCTTCTGGCGTCTCCCCCCGATGAGCAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCacc                                                                                                                          <  7:417997/138‑3 (MQ=255)
                                        gTCTCCCCCCGATGAGCAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAAtc                                                                                 <  5:89383/139‑2 (MQ=255)
                                               cccGATGAGCAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTtcat                                                                          <  5:286302/139‑1 (MQ=255)
                                               cccGATGAGCAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTtcat                                                                          <  5:346485/139‑1 (MQ=255)
                                               cccGATGAGCAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATAGTAGAAATCTGTtcat                                                                          <  7:227400/139‑1 (MQ=255)
                                               accGATGAGCAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTtcat                                                                          <  3:394844/138‑1 (MQ=255)
                                                ccGATGAGCAAGATTTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCGGCCTCCCCGGCAGGAAGGGGTTTTCCGGCACCGCACAAGTGTAGAACTCTGGtcatc                                                                         >  2:381117/1‑139 (MQ=255)
                                                    tGAGCAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCt                                                                     <  3:235779/139‑1 (MQ=255)
                                                       gCAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGt                                                                                                      <  7:196583/103‑1 (MQ=255)
                                                       gCAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGt                                                                                                      >  8:196583/1‑103 (MQ=255)
                                                       gCAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTa                                                                  >  3:300034/1‑139 (MQ=255)
                                                        cAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACaa                                                                                            <  2:99450/112‑1 (MQ=255)
                                                        cAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACaa                                                                                            >  1:99450/1‑112 (MQ=255)
                                                        cAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTa                                                                  >  2:429449/1‑138 (MQ=255)
                                                             attgACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTAtcctcc                                                            <  5:145964/136‑1 (MQ=255)
                                                                     tatcCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCATCCTTGCTAt                                                     >  4:64485/1‑138 (MQ=255)
                                                                         ccccccATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAAc                                                <  1:272308/136‑1 (MQ=255)
                                                                          cccccATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAAc                                                >  3:82886/3‑138 (MQ=255)
                                                                          cccccATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGACTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAAc                                                <  4:82886/136‑1 (MQ=255)
                                                                           ccccATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCacc                                                                                                                          >  2:150473/2‑61 (MQ=255)
                                                                           ccccATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCacc                                                                                                                          <  1:150473/62‑3 (MQ=255)
                                                                            cccATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATg                                             >  6:332884/1‑139 (MQ=255)
                                                                              cATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGtc                                           <  1:135101/139‑1 (MQ=255)
                                                                                 ttCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGtcttc                                        >  5:119277/1‑139 (MQ=255)
                                                                                           gtcATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATg                               >  5:297412/3‑138 (MQ=255)
                                                                                                        ccatcatcATAATCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTcag                   <  4:220476/135‑1 (MQ=255)
                                                                                                          atcatcATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCagcagc               >  6:117566/1‑139 (MQ=255)
                                                                                                             atcatATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGGTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCa            <  7:78882/139‑1 (MQ=255)
                                                                                                                 tatTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCgg        <  5:117566/139‑1 (MQ=255)
                                                                                                                  atTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCACCGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGc       >  7:147392/1‑139 (MQ=255)
                                                                                                                  atTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTAtcctcc                                                            >  7:84902/1‑86 (MQ=255)
                                                                                                                  atTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTAtcctcc                                                            <  8:84902/86‑1 (MQ=255)
                                                                                                                  atTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATcctcct                                                           >  6:263645/1‑87 (MQ=255)
                                                                                                                  atTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATcctcct                                                           <  5:263645/87‑1 (MQ=255)
                                                                                                                  atTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGc       >  5:430030/1‑139 (MQ=255)
                                                                                                                   ttCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGc       <  1:429449/138‑1 (MQ=255)
                                                                                                                      atcaGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTAAGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATTAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTtta   >  8:40985/1‑139 (MQ=255)
                                                                                                                        caGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAACAATGTCTTCTGCAATATGAATCGTATTCAGCAGCGCACCGGCTttat  >  6:248565/1‑138 (MQ=255)
                                                                                                                        caGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTttat  >  2:159306/1‑138 (MQ=255)
                                                                                                                         aGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTtcat                                                                          >  7:349489/1‑65 (MQ=255)
                                                                                                                         aGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTtcat                                                                          <  8:349489/65‑1 (MQ=255)
                                                                                                                             |                                                                                                                                    
TGCCCGCATCGCCTTTACAAAGCCCGTTAGCTCTTCTGGCGTCTCTCCCCGATGAGCAAGAATAGACAATATACCGCCCATTTCAGAAGGTGCCATTTCCGCTGTTATCATCATATTCATCAGCGCTTCTGCCTCATCGGCAGTAAGTGTTTTTCCGTCAACGCACAATTGTAGAAATTTGTTCATCAGCTTTATCCTCCTTGCTATGGAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTAT  >  CP006881/2193577‑2193834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑