Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,193,724	T→G	T13T (ACA→ACC)	U712_10990 ←	Anthranilate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,193,724	0	T	G	100.0%	161.7 / NA	50	T13T (ACA→ACC)	U712_10990	Anthranilate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (26/24);  total (26/24)

GTCTCTCCCCGATGAGCAAGAATAGACAATATACCGCCCATTTCAGAAGGTGCCATTTCCGCTGTTATCATCATATTCATCAGCGCTTCTGCCTCATCGGCAGTAAGTGTTTTTCCGTCAACGCACAATTGTAGAAATTTGTTCATCAGCTTTATCCTCCTTGCTATGGAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTC  >  CP006881/2193617‑2193859                                                                                                            |                                                                                                                                        gTCTCCCCCCGATGAGCAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAAtc                                                                                                          <  5:89383/139‑2 (MQ=255)        cccGATGAGCAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTtcat                                                                                                   <  5:286302/139‑1 (MQ=255)        cccGATGAGCAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTtcat                                                                                                   <  5:346485/139‑1 (MQ=255)        cccGATGAGCAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATAGTAGAAATCTGTtcat                                                                                                   <  7:227400/139‑1 (MQ=255)        accGATGAGCAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTtcat                                                                                                   <  3:394844/138‑1 (MQ=255)         ccGATGAGCAAGATTTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCGGCCTCCCCGGCAGGAAGGGGTTTTCCGGCACCGCACAAGTGTAGAACTCTGGtcatc                                                                                                  >  2:381117/1‑139 (MQ=255)             tGAGCAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCt                                                                                              <  3:235779/139‑1 (MQ=255)                gCAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGt                                                                                                                               <  7:196583/103‑1 (MQ=255)                gCAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGt                                                                                                                               >  8:196583/1‑103 (MQ=255)                gCAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTa                                                                                           >  3:300034/1‑139 (MQ=255)                 cAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACaa                                                                                                                     >  1:99450/1‑112 (MQ=255)                 cAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACaa                                                                                                                     <  2:99450/112‑1 (MQ=255)                 cAAGAATTGACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTa                                                                                           >  2:429449/1‑138 (MQ=255)                      attgACAATATCCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTAtcctcc                                                                                     <  5:145964/136‑1 (MQ=255)                              tatcCCCCCCATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCATCCTTGCTAt                                                                              >  4:64485/1‑138 (MQ=255)                                  ccccccATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAAc                                                                         <  1:272308/136‑1 (MQ=255)                                   cccccATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAAc                                                                         >  3:82886/3‑138 (MQ=255)                                   cccccATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGACTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAAc                                                                         <  4:82886/136‑1 (MQ=255)                                     cccATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATg                                                                      >  6:332884/1‑139 (MQ=255)                                       cATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGtc                                                                    <  1:135101/139‑1 (MQ=255)                                          ttCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGtcttc                                                                 >  5:119277/1‑139 (MQ=255)                                                    gtcATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATg                                                        >  5:297412/3‑138 (MQ=255)                                                                 ccatcatcATAATCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTcag                                            <  4:220476/135‑1 (MQ=255)                                                                   atcatcATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCagcagc                                        >  6:117566/1‑139 (MQ=255)                                                                      atcatATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGGTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCa                                     <  7:78882/139‑1 (MQ=255)                                                                          tatTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCgg                                 <  5:117566/139‑1 (MQ=255)                                                                           atTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCACCGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGc                                >  7:147392/1‑139 (MQ=255)                                                                           atTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTAtcctcc                                                                                     >  7:84902/1‑86 (MQ=255)                                                                           atTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTAtcctcc                                                                                     <  8:84902/86‑1 (MQ=255)                                                                           atTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATcctcct                                                                                    >  6:263645/1‑87 (MQ=255)                                                                           atTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATcctcct                                                                                    <  5:263645/87‑1 (MQ=255)                                                                           atTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGc                                >  5:430030/1‑139 (MQ=255)                                                                            ttCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGc                                <  1:429449/138‑1 (MQ=255)                                                                               atcaGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTAAGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATTAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTtta                            >  8:40985/1‑139 (MQ=255)                                                                                 caGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAACAATGTCTTCTGCAATATGAATCGTATTCAGCAGCGCACCGGCTttat                           >  6:248565/1‑138 (MQ=255)                                                                                 caGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTttat                           >  2:159306/1‑138 (MQ=255)                                                                                  aGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTtcat                                                                                                   <  8:349489/65‑1 (MQ=255)                                                                                  aGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTtcat                                                                                                   >  7:349489/1‑65 (MQ=255)                                                                                    cgtttCAGCCTCACCGTCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTttatta                         >  1:341254/4‑137 (MQ=255)                                                                                    cgtttCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCAcc                                   <  8:436879/124‑1 (MQ=255)                                                                                    cgtttCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCAcc                                   >  7:436879/4‑127 (MQ=255)                                                                                    cgtttCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGc                                >  1:366691/4‑130 (MQ=255)                                                                                    cgtttCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGc                                <  2:366691/127‑1 (MQ=255)                                                                                    cgtttCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCCttatt                          >  1:239527/4‑136 (MQ=255)                                                                                    cgtttCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCCttatt                          <  2:233228/133‑1 (MQ=255)                                                                                    cgtttCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCCttatt                          <  2:239527/133‑1 (MQ=255)                                                                                    cgtttCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCCttatt                          >  1:233228/4‑136 (MQ=255)                                                                                           gCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTttct                <  1:356459/139‑1 (MQ=255)                                                                                                accGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTa           >  6:61469/3‑139 (MQ=255)                                                                                                         aaGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTc  >  8:336786/1‑139 (MQ=255)                                                                                                            |                                                                                                                                        GTCTCTCCCCGATGAGCAAGAATAGACAATATACCGCCCATTTCAGAAGGTGCCATTTCCGCTGTTATCATCATATTCATCAGCGCTTCTGCCTCATCGGCAGTAAGTGTTTTTCCGTCAACGCACAATTGTAGAAATTTGTTCATCAGCTTTATCCTCCTTGCTATGGAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTC  >  CP006881/2193617‑2193859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑