Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,193,785	G→A	F499F (TTC→TTT)	U712_10995 ←	Anthranilate synthase component 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,193,785	0	G	A	100.0%	201.0 / NA	64	F499F (TTC→TTT)	U712_10995	Anthranilate synthase component 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/31);  total (33/31)

CCGCCCATTTCAGAAGGTGCCATTTCCGCTGTTATCATCATATTCATCAGCGCTTCTGCCTCATCGGCAGTAAGTGTTTTTCCGTCAACGCACAATTGTAGAAATTTGTTCATCAGCTTTATCCTCCTTGCTATGGAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTCCGGAACAGAATCCGCAACAATTCCAGCACCTGCCTGTATCGATGCAACACCGTTCTT  >  CP006881/2193650‑2193916                                                                                                                                        |                                                                                                                                    ccccccATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAAc                                                                                                                                  <  1:272308/136‑1 (MQ=255)  cccccATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAAc                                                                                                                                  >  3:82886/3‑138 (MQ=255)  cccccATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGACTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAAc                                                                                                                                  <  4:82886/136‑1 (MQ=255)    cccATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATg                                                                                                                               >  6:332884/1‑139 (MQ=255)      cATTTCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGtc                                                                                                                             <  1:135101/139‑1 (MQ=255)         ttCAGAAGGAGTCATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGtcttc                                                                                                                          >  5:119277/1‑139 (MQ=255)                   gtcATTTCCGCTGCCATCATCATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATg                                                                                                                 >  5:297412/3‑138 (MQ=255)                                ccatcatcATAATCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTcag                                                                                                     <  4:220476/135‑1 (MQ=255)                                  atcatcATATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCagcagc                                                                                                 >  6:117566/1‑139 (MQ=255)                                     atcatATTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGGTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCa                                                                                              <  7:78882/139‑1 (MQ=255)                                         tatTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCgg                                                                                          <  5:117566/139‑1 (MQ=255)                                          atTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCACCGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGc                                                                                         >  7:147392/1‑139 (MQ=255)                                          atTCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGc                                                                                         >  5:430030/1‑139 (MQ=255)                                           ttCATCAGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGc                                                                                         <  1:429449/138‑1 (MQ=255)                                              atcaGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTAAGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATTAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTtta                                                                                     >  8:40985/1‑139 (MQ=255)                                                caGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAACAATGTCTTCTGCAATATGAATCGTATTCAGCAGCGCACCGGCTttat                                                                                    >  6:248565/1‑138 (MQ=255)                                                caGCGTTTCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTttat                                                                                    >  2:159306/1‑138 (MQ=255)                                                   cgtttCAGCCTCACCGTCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTttatta                                                                                  >  1:341254/4‑137 (MQ=255)                                                   cgtttCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCAcc                                                                                            >  7:436879/4‑127 (MQ=255)                                                   cgtttCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCAcc                                                                                            <  8:436879/124‑1 (MQ=255)                                                   cgtttCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGc                                                                                         <  2:366691/127‑1 (MQ=255)                                                   cgtttCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGc                                                                                         >  1:366691/4‑130 (MQ=255)                                                   cgtttCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCCttatt                                                                                   <  2:239527/133‑1 (MQ=255)                                                   cgtttCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCCttatt                                                                                   <  2:233228/133‑1 (MQ=255)                                                   cgtttCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCCttatt                                                                                   >  1:239527/4‑136 (MQ=255)                                                   cgtttCAGCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCCttatt                                                                                   >  1:233228/4‑136 (MQ=255)                                                          gCCTCACCGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTttct                                                                         <  1:356459/139‑1 (MQ=255)                                                               accGGCAGTAAGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTa                                                                    >  6:61469/3‑139 (MQ=255)                                                                        aaGGGTTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTc                                                           >  8:336786/1‑139 (MQ=255)                                                                         agggtTTTTCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTcc                                                          >  4:127730/4‑139 (MQ=255)                                                                             tttttCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTCCGGaa                                                      <  7:304678/139‑1 (MQ=255)                                                                               tttCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATc                                                                                                             <  4:211097/82‑1 (MQ=255)                                                                               tttCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATc                                                                                                             >  3:211097/1‑82 (MQ=255)                                                                               tttCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTCCGGaa                                                      >  4:340055/1‑137 (MQ=255)                                                                                 tCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTCCGGAACAg                                                   >  1:280114/1‑138 (MQ=255)                                                                                 tCCGTCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTGTCTGCAGCGCACCGGCTTTATTCCAGCTTTCTTCGTATTCGGCTTCCGGAACAg                                                   >  4:14199/1‑138 (MQ=255)                                                                                     tCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGaa                                                                                                               >  5:102573/1‑74 (MQ=255)                                                                                     tCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGaa                                                                                                               <  6:102573/74‑1 (MQ=255)                                                                                     tCAACGCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTCCGGAACAGAAt                                                >  5:21226/1‑137 (MQ=255)                                                                                          gCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTCCGGAACAGAATCAGcaac                                         <  5:297272/139‑1 (MQ=255)                                                                                          gCACAATTGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTCCGGAACAGAATCAGcaac                                         <  3:20202/139‑1 (MQ=255)                                                                                                ttGTAGAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTCCGGAACAGAATCAGCAACAAtgc                                    >  3:179892/1‑136 (MQ=255)                                                                                                     gAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTCCGGAACAGAATCAGCAACAATGCCAGCa                               <  2:280114/138‑1 (MQ=255)                                                                                                     gAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTCCGGAACAGAATCAGCAACAATGCCAGCAc                              >  5:113872/1‑139 (MQ=255)                                                                                                     gAAATCTGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTCCGGAACAGAATCAGCAACAATGCCAGCAc                              <  5:432291/139‑1 (MQ=255)                                                                                                     gAAATCTGAGCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTCCGGAACAGAATCAGCAACAATGCCAGCAc                              <  7:200868/139‑1 (MQ=255)                                                                                                        atctGTTCATCAGCTTAATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTAATTACAGCTTTCTTCGTATTCGGCTTCCGGAACAGAATCAGCAACAAAGCCCGCAactg                           >  6:432291/4‑139 (MQ=255)                                                                                                          ctGTTCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTCCGGAACAGAATCAGCAACAATGCCAGCAcctgcc                         >  3:258121/2‑139 (MQ=255)                                                                                                             ttCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTCCGGAACAGAATCAGCAACAATGCCAGCAcctgcctg                       >  3:65723/1‑138 (MQ=255)                                                                                                             ttCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTCCGGAACAGAATCAGCAACAATGCCAGCAcctgc                          <  4:258121/135‑1 (MQ=255)                                                                                                             ttCATCAGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTCCGGAACAGAATCAGCAACAATGCCAGCAcctgc                          <  3:64485/135‑1 (MQ=255)                                                                                                                atcaGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTCCGGAACAGAATCAGCAACAATGCCAGCACCTGCCTGTAt                    <  6:430030/138‑1 (MQ=255)                                                                                                                atcaGCTTTATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTCCGGAACAGAATCAGCAACAATGCCAGCACCTGCCTGTAt                    <  3:340055/138‑1 (MQ=255)                                                                                                                       ttATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTCCGGAACAGAATCAGCAACAATGCCAGCACCTGCCTGTAt                    <  6:266240/131‑1 (MQ=255)                                                                                                                       ttATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTCCGGAACAGAATCAGCAACAATGCAAGCACCTGCCTGTAt                    >  5:266240/1‑131 (MQ=255)                                                                                                                        tATCCTCCTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTCCGGAACAGAATCAGCAACAATGCCAGCACCTGCCTGTATCGATGCaa            <  6:21226/138‑1 (MQ=255)                                                                                                                          tcctccTTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTCCGGAACAGAATCAGCAACAATGCCAGCACCTGCCTGTATCGATGCAaca          <  5:248565/138‑1 (MQ=255)                                                                                                                            ctcctTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTCCGGAACAGAATCAGCAACAATGCCAGCACCTGCCTGTATCGATGCAACAcc        <  1:159306/138‑1 (MQ=255)                                                                                                                            ctcctTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTCCGGAACAGAATCAGCAACAATGCCAGCACCTGCCTGTATCGATGCAACAcc        <  1:381117/138‑1 (MQ=255)                                                                                                                            ctcctTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTCCGGAACAGAATCAGCAACAATGCCAGCACCTGCCTGTATCGATGCAACAcc        <  5:332884/138‑1 (MQ=255)                                                                                                                            ctcctTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTCCGGAACAGAATCAGCAACAATGCCAGCACCTGCCTGTATCGATGCAACAcc        <  6:119277/138‑1 (MQ=255)                                                                                                                              cctTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTCCGGAACAGAATCAGCAACAATGCCAGCACCTGCCTGTATCGATGCAACACCGtt     >  7:442436/1‑139 (MQ=255)                                                                                                                               ctTGCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTCCGGAACAGAATCAGCAACAATGCCAGCACCTGCCTGTATCGATGCAACACCGTTc    >  7:74266/1‑139 (MQ=255)                                                                                                                                  gCTATGAAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTCCGGAACAGAATCAGCAACAATGCCAGCACCTGCCTGTATCGATGCAACACCGTTCtt  >  7:204017/1‑138 (MQ=255)                                                                                                                                        |                                                                                                                                    CCGCCCATTTCAGAAGGTGCCATTTCCGCTGTTATCATCATATTCATCAGCGCTTCTGCCTCATCGGCAGTAAGTGTTTTTCCGTCAACGCACAATTGTAGAAATTTGTTCATCAGCTTTATCCTCCTTGCTATGGAACATGTCTTCTGCAATATGAATCGTTTTCAGCAGCGCACCGGCTTTATTACAGCTTTCTTCGTATTCGGCTTCCGGAACAGAATCCGCAACAATTCCAGCACCTGCCTGTATCGATGCAACACCGTTCTT  >  CP006881/2193650‑2193916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑