Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,194,292 G→C L330L (CTC→CTG)  U712_10995 ← Anthranilate synthase component 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,194,2920GC98.6% 214.4 / NA 71L330L (CTC→CTG) U712_10995Anthranilate synthase component 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (30/40);  minor base A (1/0);  total (31/40)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGAGACAATTTTTGTGAACTCCGGCACAGAAACAGATCCATACTCTGCTACTCTGCCAATATCGTTCCGGGCAAGGTCAACGAGCATGTAGTGCTCCGCTTTTTCTTTTTCATCCTTCATCAGCTCAACTTTGAGTTTTTCATCTTCCTCTTTATCTGCGCCTCGTTTTCTCGTACCGGCAATCGGATGGATTTCCAAGTGCCCGTCCTTAACGTGGATTAATCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTTGGAGTT  >  CP006881/2194160‑2194422
                                                                                                                                    |                                                                                                                                  
ggAAACAATTTTTGTGAACTCCGGCACAGAAACAGAACCATACTCTGCTACTCTGCCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGtctc                                                                                                                              <  4:15904/139‑4 (MQ=255)
    aCAATTTTTGTGAACTCCGGCACAGAAACAGAACCAGACTCTGCTACTCTGCCGATATCGTTTCGGGCAAGATCACCGCGCATGTAATGCTCCGCTTTTTCTTGTTCATCCTTCATTAGCTCAACCTTCAGTCTCTCAt                                                                                                                          >  8:132112/1‑139 (MQ=255)
       aTTTTTGTGAACTCCGGCACAGAAACAGAACCATACTCTGCTACTCTGCCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCtt                                                                                                                       <  6:162521/139‑1 (MQ=255)
           ttGTGAACTCCGGCACAGAAACAGAACCATACTCTGCTACTCTGCCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATGCTTCATGAGCTCAACCGTCAGTCTCTCATCTTcagc                                                                                                                   >  1:271697/1‑136 (MQ=255)
            tgtgAACTCCGGCACAGAAACAGAACCATACTCTGCTACTCTGCCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTcagc                                                                                                                   <  8:144396/138‑4 (MQ=255)
                aaCTCCGGCACAGAAACAGAACCATACTCTGCTACTCTGCCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCtttg                                                                                                               >  2:351026/1‑137 (MQ=255)
                          caGAAACAGAACCATACTCTGCTACTCTGCCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACcgcg                                                                                                    <  7:356627/139‑1 (MQ=255)
                                        tactctgctactctgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTTCCgg                                                                                      >  4:288538/1‑139 (MQ=255)
                                        tactctgctactctgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCgg                                                                                      >  4:356414/1‑139 (MQ=255)
                                        tactctgctactctgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCCTTTTCTCGTACCgg                                                                                      >  3:185731/1‑139 (MQ=255)
                                          ctctgctactctgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCa                                                                                    <  8:240689/139‑1 (MQ=255)
                                             tgctactctgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTGAACCTTCAGTCTGTCATCTTCAGCGTTGTCTGCACCGCGTTTTGTCGTACCGGCAATc                                                                                 >  1:414981/1‑139 (MQ=255)
                                             tgctactctgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATc                                                                                 >  7:356378/1‑139 (MQ=255)
                                             tgctactctgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATc                                                                                 >  1:410525/1‑139 (MQ=255)
                                             tgctactctgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATc                                                                                 >  5:308630/1‑139 (MQ=255)
                                             tgctactctgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATc                                                                                 >  4:444909/1‑139 (MQ=255)
                                             tgctactctgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGGTTTCTCGTACCGGCaagc                                                                                 >  4:393544/1‑137 (MQ=255)
                                             tgctactctgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCGTCATGCGCTCAACCTTCAGTCTCTCATATTCAGATTTGTCTGCCCAGCGGTTTCTCGTACCCGCAATc                                                                                 >  6:341717/1‑139 (MQ=255)
                                               ctactctgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCgg                                                                               >  7:381119/1‑139 (MQ=255)
                                               ctactctgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCATTCATGAGCTCAACCAAAAGTCTCACCTCTTCACCATTGTCAGCACCGCGGTTTCTCGTACCGGCAATCg                                                                                >  6:399231/1‑138 (MQ=255)
                                                  ctctgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCggat                                                                             <  1:214442/138‑1 (MQ=255)
                                                  ctctgcCGATATCGTTTCGGGCAAGACCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCggat                                                                             <  8:210069/138‑1 (MQ=255)
                                                     tgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTGATGGGCTCAACCTTCAGTCGCTCATCTTCAGCTTGTTCTGCCCCGCGTTTTCTCGTACCGGCAATCggatggat                                                                         >  8:99793/1‑139 (MQ=255)
                                                     tgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCggatggat                                                                         >  6:44082/1‑139 (MQ=255)
                                                       ccgatatCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCCCCGCGTTTTCTCGTACCGGCCATCGGATGGAttt                                                                       >  5:136322/4‑139 (MQ=255)
                                                       ccgatatCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCggat                                                                             >  3:45220/4‑133 (MQ=255)
                                                       ccgatatCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCggat                                                                             <  4:45220/130‑1 (MQ=255)
                                                         gatatCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTc                                                                      >  7:36179/2‑138 (MQ=255)
                                                               gTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGc                                                               <  7:54837/139‑1 (MQ=255)
                                                               gTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGc                                                               <  4:363945/139‑1 (MQ=255)
                                                                tttcGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGcc                                                              <  5:394070/136‑1 (MQ=255)
                                                                tttcGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGcc                                                              <  2:410525/136‑1 (MQ=255)
                                                                tttcGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGcc                                                              <  8:236253/136‑1 (MQ=255)
                                                                tttcGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGcc                                                              <  8:51741/136‑1 (MQ=255)
                                                                          gatccaCGAGCATGTAATGCTCCGCTTTATCTTATTCATCCTTCCTGAGCTCAACCTTCAGTCTCTCATCATCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAAc                                                    <  8:416202/134‑1 (MQ=255)
                                                                          gatCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAAc                                                    <  4:214434/137‑1 (MQ=255)
                                                                          gatCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAAc                                                    <  8:356378/137‑1 (MQ=255)
                                                                          gatCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAAc                                                    <  3:444909/137‑1 (MQ=255)
                                                                                   gCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTaa                                           <  4:268753/139‑1 (MQ=255)
                                                                                   gCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTaa                                           <  3:418785/139‑1 (MQ=255)
                                                                                      tGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTaa                                           >  4:343577/1‑136 (MQ=255)
                                                                                      tGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTaa                                           <  3:343577/136‑1 (MQ=255)
                                                                                      tGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCg                                        >  7:175168/1‑139 (MQ=255)
                                                                                           tGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCg                                   <  3:22666/139‑1 (MQ=255)
                                                                                             cTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCGTCAGGCTCTCCTCGTCAGCTTTGTCTGCACCGCGTTTTATCGTAACGGCCATAGGATGGTTTTATAACTGGCCGTCTTTCACGTGTATTAACCGTTCCg                                   >  6:95098/1‑137 (MQ=255)
                                                                                               ccGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCATCAGTCTCTCATCTTCACCTTTTTATGCAGCGCGTTTTCTCGTACCGGCAATCGGATGGAGATCTAAGTGCCCCTCTTTAACGTGTATTAACCGTTCCGga                                 >  6:74668/1‑137 (MQ=255)
                                                                                                cGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGGTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGagag                              >  7:98427/1‑139 (MQ=255)
                                                                                                cGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTATCTCATCTTCAGCGTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGgag                                <  5:341717/137‑1 (MQ=255)
                                                                                                  ctttttctttttCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCCCCGCGTTTTCTCGTACCGGCAATCGGATGGGTTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTGCGGAGAGCt                            >  8:365599/1‑139 (MQ=255)
                                                                                                  ctttttctttttCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTcttgaa                                                     <  2:151222/114‑6 (MQ=255)
                                                                                                  ctttttctttttCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTcttgaa                                                     >  1:151222/1‑109 (MQ=255)
                                                                                                      ttctttttcATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGcc                         <  8:381119/138‑1 (MQ=255)
                                                                                                       tctttttcATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTG‑CCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGa                       <  3:222598/138‑1 (MQ=255)
                                                                                                              cATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTAttt                 <  3:242312/138‑1 (MQ=255)
                                                                                                               aTCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTAGCGGCAATCTGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTtct               <  7:178661/139‑1 (MQ=255)
                                                                                                               aTCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTtct               <  2:301596/139‑1 (MQ=255)
                                                                                                               aTCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTtct               <  8:140055/139‑1 (MQ=255)
                                                                                                               aTCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTtct               <  6:308630/139‑1 (MQ=255)
                                                                                                               aTCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTtct               <  2:414981/139‑1 (MQ=255)
                                                                                                               aTCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTtct               <  8:36179/139‑1 (MQ=255)
                                                                                                               aTCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTtct               <  8:362887/139‑1 (MQ=255)
                                                                                                               aTCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCGTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTtct               <  5:399231/139‑1 (MQ=255)
                                                                                                               aTCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCGTTGGCTGCACCGCGTGTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTtct               <  2:189173/139‑1 (MQ=255)
                                                                                                               aTCCTTCATGAGCTCAACCTTCAGGCTCTCATCTTCAGCTTTGTCGGCACCGCGGTTTCTCGTACCGGCAATCGGATGGATTGCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTtct               <  2:164699/139‑1 (MQ=255)
                                                                                                                     catgaGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTtgtctgtcg         >  1:75118/1‑138 (MQ=255)
                                                                                                                       tgaGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCTGCAATCGGATTGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGTAGAGCTTCCGACTATTTCTCTGTCtag       <  5:381938/137‑3 (MQ=255)
                                                                                                                           cTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGCCGATTTgtctgtctagcagg   >  6:193414/1‑133 (MQ=255)
                                                                                                                           cTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGt   >  3:334737/1‑139 (MQ=255)
                                                                                                                           cTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGt   >  4:211560/1‑139 (MQ=255)
                                                                                                                              aaCCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGtt  <  7:441556/137‑1 (MQ=255)
                                                                                                                              aaCCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGAGTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGtt  <  7:132112/137‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                  
GGAGACAATTTTTGTGAACTCCGGCACAGAAACAGATCCATACTCTGCTACTCTGCCAATATCGTTCCGGGCAAGGTCAACGAGCATGTAGTGCTCCGCTTTTTCTTTTTCATCCTTCATCAGCTCAACTTTGAGTTTTTCATCTTCCTCTTTATCTGCGCCTCGTTTTCTCGTACCGGCAATCGGATGGATTTCCAAGTGCCCGTCCTTAACGTGGATTAATCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTTGGAGTT  >  CP006881/2194160‑2194422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑