Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,194,296 T→C K329R (AAA→AGA)  U712_10995 ← Anthranilate synthase component 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,194,2960TC98.6% 216.9 / NA 70K329R (AAA→AGA) U712_10995Anthranilate synthase component 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (31/38);  minor base A (0/1);  total (31/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACAATTTTTGTGAACTCCGGCACAGAAACAGATCCATACTCTGCTACTCTGCCAATATCGTTCCGGGCAAGGTCAACGAGCATGTAGTGCTCCGCTTTTTCTTTTTCATCCTTCATCAGCTCAACTTTGAGTTTTTCATCTTCCTCTTTATCTGCGCCTCGTTTTCTCGTACCGGCAATCGGATGGATTTCCAAGTGCCCGTCCTTAACGTGGATTAATCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTTGGAGTT  >  CP006881/2194164‑2194422
                                                                                                                                    |                                                                                                                              
aCAATTTTTGTGAACTCCGGCACAGAAACAGAACCAGACTCTGCTACTCTGCCGATATCGTTTCGGGCAAGATCACCGCGCATGTAATGCTCCGCTTTTTCTTGTTCATCCTTCATTAGCTCAACCTTCAGTCTCTCAt                                                                                                                          >  8:132112/1‑139 (MQ=255)
   aTTTTTGTGAACTCCGGCACAGAAACAGAACCATACTCTGCTACTCTGCCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCtt                                                                                                                       <  6:162521/139‑1 (MQ=255)
       ttGTGAACTCCGGCACAGAAACAGAACCATACTCTGCTACTCTGCCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATGCTTCATGAGCTCAACCGTCAGTCTCTCATCTTcagc                                                                                                                   >  1:271697/1‑136 (MQ=255)
        tgtgAACTCCGGCACAGAAACAGAACCATACTCTGCTACTCTGCCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTcagc                                                                                                                   <  8:144396/138‑4 (MQ=255)
            aaCTCCGGCACAGAAACAGAACCATACTCTGCTACTCTGCCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCtttg                                                                                                               >  2:351026/1‑137 (MQ=255)
                      caGAAACAGAACCATACTCTGCTACTCTGCCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACcgcg                                                                                                    <  7:356627/139‑1 (MQ=255)
                                    tactctgctactctgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTTCCgg                                                                                      >  4:288538/1‑139 (MQ=255)
                                    tactctgctactctgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCgg                                                                                      >  4:356414/1‑139 (MQ=255)
                                    tactctgctactctgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCCTTTTCTCGTACCgg                                                                                      >  3:185731/1‑139 (MQ=255)
                                      ctctgctactctgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCa                                                                                    <  8:240689/139‑1 (MQ=255)
                                         tgctactctgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTGAACCTTCAGTCTGTCATCTTCAGCGTTGTCTGCACCGCGTTTTGTCGTACCGGCAATc                                                                                 >  1:414981/1‑139 (MQ=255)
                                         tgctactctgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATc                                                                                 >  1:410525/1‑139 (MQ=255)
                                         tgctactctgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATc                                                                                 >  7:356378/1‑139 (MQ=255)
                                         tgctactctgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATc                                                                                 >  5:308630/1‑139 (MQ=255)
                                         tgctactctgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATc                                                                                 >  4:444909/1‑139 (MQ=255)
                                         tgctactctgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGGTTTCTCGTACCGGCaagc                                                                                 >  4:393544/1‑137 (MQ=255)
                                         tgctactctgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCGTCATGCGCTCAACCTTCAGTCTCTCATATTCAGATTTGTCTGCCCAGCGGTTTCTCGTACCCGCAATc                                                                                 >  6:341717/1‑139 (MQ=255)
                                           ctactctgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCgg                                                                               >  7:381119/1‑139 (MQ=255)
                                           ctactctgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCATTCATGAGCTCAACCAAAAGTCTCACCTCTTCACCATTGTCAGCACCGCGGTTTCTCGTACCGGCAATCg                                                                                >  6:399231/1‑138 (MQ=255)
                                              ctctgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCggat                                                                             <  1:214442/138‑1 (MQ=255)
                                              ctctgcCGATATCGTTTCGGGCAAGACCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCggat                                                                             <  8:210069/138‑1 (MQ=255)
                                                 tgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTGATGGGCTCAACCTTCAGTCGCTCATCTTCAGCTTGTTCTGCCCCGCGTTTTCTCGTACCGGCAATCggatggat                                                                         >  8:99793/1‑139 (MQ=255)
                                                 tgcCGATATCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCggatggat                                                                         >  6:44082/1‑139 (MQ=255)
                                                   ccgatatCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCCCCGCGTTTTCTCGTACCGGCCATCGGATGGAttt                                                                       >  5:136322/4‑139 (MQ=255)
                                                   ccgatatCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCggat                                                                             >  3:45220/4‑133 (MQ=255)
                                                   ccgatatCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCggat                                                                             <  4:45220/130‑1 (MQ=255)
                                                     gatatCGTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTc                                                                      >  7:36179/2‑138 (MQ=255)
                                                           gTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGc                                                               <  7:54837/139‑1 (MQ=255)
                                                           gTTTCGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGc                                                               <  4:363945/139‑1 (MQ=255)
                                                            tttcGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGcc                                                              <  5:394070/136‑1 (MQ=255)
                                                            tttcGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGcc                                                              <  2:410525/136‑1 (MQ=255)
                                                            tttcGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGcc                                                              <  8:236253/136‑1 (MQ=255)
                                                            tttcGGGCAAGATCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGcc                                                              <  8:51741/136‑1 (MQ=255)
                                                                      gatccaCGAGCATGTAATGCTCCGCTTTATCTTATTCATCCTTCCTGAGCTCAACCTTCAGTCTCTCATCATCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAAc                                                    <  8:416202/134‑1 (MQ=255)
                                                                      gatCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAAc                                                    <  4:214434/137‑1 (MQ=255)
                                                                      gatCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAAc                                                    <  8:356378/137‑1 (MQ=255)
                                                                      gatCAACGAGCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAAc                                                    <  3:444909/137‑1 (MQ=255)
                                                                               gCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTaa                                           <  4:268753/139‑1 (MQ=255)
                                                                               gCATGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTaa                                           <  3:418785/139‑1 (MQ=255)
                                                                                  tGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTaa                                           >  4:343577/1‑136 (MQ=255)
                                                                                  tGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTaa                                           <  3:343577/136‑1 (MQ=255)
                                                                                  tGTAATGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCg                                        >  7:175168/1‑139 (MQ=255)
                                                                                       tGCTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCg                                   <  3:22666/139‑1 (MQ=255)
                                                                                         cTCCGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCGTCAGGCTCTCCTCGTCAGCTTTGTCTGCACCGCGTTTTATCGTAACGGCCATAGGATGGTTTTATAACTGGCCGTCTTTCACGTGTATTAACCGTTCCg                                   >  6:95098/1‑137 (MQ=255)
                                                                                           ccGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCATCAGTCTCTCATCTTCACCTTTTTATGCAGCGCGTTTTCTCGTACCGGCAATCGGATGGAGATCTAAGTGCCCCTCTTTAACGTGTATTAACCGTTCCGga                                 >  6:74668/1‑137 (MQ=255)
                                                                                            cGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGGTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGagag                              >  7:98427/1‑139 (MQ=255)
                                                                                            cGCTTTTTCTTTTTCATCCTTCATGAGCTCAACCTTCAGTATCTCATCTTCAGCGTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGgag                                <  5:341717/137‑1 (MQ=255)
                                                                                              ctttttctttttCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCCCCGCGTTTTCTCGTACCGGCAATCGGATGGGTTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTGCGGAGAGCt                            >  8:365599/1‑139 (MQ=255)
                                                                                              ctttttctttttCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTcttgaa                                                     <  2:151222/114‑6 (MQ=255)
                                                                                              ctttttctttttCATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTcttgaa                                                     >  1:151222/1‑109 (MQ=255)
                                                                                                  ttctttttcATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGcc                         <  8:381119/138‑1 (MQ=255)
                                                                                                   tctttttcATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTG‑CCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGa                       <  3:222598/138‑1 (MQ=255)
                                                                                                          cATCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTAttt                 <  3:242312/138‑1 (MQ=255)
                                                                                                           aTCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTAGCGGCAATCTGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTtct               <  7:178661/139‑1 (MQ=255)
                                                                                                           aTCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTtct               <  2:301596/139‑1 (MQ=255)
                                                                                                           aTCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTtct               <  8:140055/139‑1 (MQ=255)
                                                                                                           aTCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTtct               <  6:308630/139‑1 (MQ=255)
                                                                                                           aTCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTtct               <  2:414981/139‑1 (MQ=255)
                                                                                                           aTCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTtct               <  8:36179/139‑1 (MQ=255)
                                                                                                           aTCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTtct               <  8:362887/139‑1 (MQ=255)
                                                                                                           aTCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCGTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTtct               <  5:399231/139‑1 (MQ=255)
                                                                                                           aTCCTTCATGAGCTCAACCTTCAGTCTCTCATCTTCAGCGTTGGCTGCACCGCGTGTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTtct               <  2:189173/139‑1 (MQ=255)
                                                                                                           aTCCTTCATGAGCTCAACCTTCAGGCTCTCATCTTCAGCTTTGTCGGCACCGCGGTTTCTCGTACCGGCAATCGGATGGATTGCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTtct               <  2:164699/139‑1 (MQ=255)
                                                                                                                 catgaGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTtgtctgtcg         >  1:75118/1‑138 (MQ=255)
                                                                                                                   tgaGCTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCTGCAATCGGATTGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGTAGAGCTTCCGACTATTTCTCTGTCtag       <  5:381938/137‑3 (MQ=255)
                                                                                                                       cTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGCCGATTTgtctgtctagcagg   >  6:193414/1‑133 (MQ=255)
                                                                                                                       cTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGt   >  3:334737/1‑139 (MQ=255)
                                                                                                                       cTCAACCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGt   >  4:211560/1‑139 (MQ=255)
                                                                                                                          aaCCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGtt  <  7:441556/137‑1 (MQ=255)
                                                                                                                          aaCCTTCAGTCTCTCATCTTCAGCTTTGTCTGCACCGCGTTTTCTCGTACCGGCAATCGGATGGAGTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGtt  <  7:132112/137‑1 (MQ=255)
                                                                                                                                    |                                                                                                                              
ACAATTTTTGTGAACTCCGGCACAGAAACAGATCCATACTCTGCTACTCTGCCAATATCGTTCCGGGCAAGGTCAACGAGCATGTAGTGCTCCGCTTTTTCTTTTTCATCCTTCATCAGCTCAACTTTGAGTTTTTCATCTTCCTCTTTATCTGCGCCTCGTTTTCTCGTACCGGCAATCGGATGGATTTCCAAGTGCCCGTCCTTAACGTGGATTAATCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTTGGAGTT  >  CP006881/2194164‑2194422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑