| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 2,194,442 | A→C | S280S (TCT→TCG) | U712_10995 ← | Anthranilate synthase component 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,194,442 | 0 | A | C | 96.7% | 189.8 / NA | 60 | S280S (TCT→TCG) | U712_10995 | Anthranilate synthase component 1 |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (28/30); total (29/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCCTCGTTTTCTCGTACCGGCAATCGGATGGATTTCCAAGTGCCCGTCCTTAACGTGGATTAATCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTTGGAGTTTCATATAATACATATACGGAGAAGGATTGACGATCCTAAGCACCCGGTATAACTCAAAGGCATCTGATTTTATCGGAATCTCAAATTTTTGTGACAGAACGCCTTGGAAGATATCGCCCGCTTTTATATATTCTTTTATTTTTTCTACATCAG > CP006881/2194318‑2194575 | caccgcGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACg < 8:175168/132‑1 (MQ=255)caccgcGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACg < 3:288538/132‑1 (MQ=255)caccgcGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACg < 3:393544/132‑1 (MQ=255)caccgcGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACg < 7:99793/132‑1 (MQ=255) accgcGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATc > 8:290606/5‑139 (MQ=255) cGTTTTCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCt > 2:279980/1‑137 (MQ=255) ttCTCGTACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCa < 6:136322/138‑1 (MQ=255) tACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGta < 6:41292/139‑1 (MQ=255) tACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGta < 7:290606/139‑1 (MQ=255) ccGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGtat < 3:356414/138‑1 (MQ=255) cGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATaa < 2:271697/139‑1 (MQ=255) gCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACt > 3:314034/1‑139 (MQ=255) tggatTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCAt < 5:193414/139‑1 (MQ=255) tggatTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCAt < 1:279980/139‑1 (MQ=255) tggatTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCAt < 3:211560/139‑1 (MQ=255) gCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGa > 4:275747/1‑93 (MQ=255) gCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGa < 3:275747/93‑1 (MQ=255) gCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCa > 7:46224/1‑105 (MQ=255) gCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCa < 8:46224/105‑1 (MQ=255) tgtaTTAACCGATCCGGAGAGCTGCCGACTATTTCTATGTATAGCAGTTTCATAATATACATATACGGATAAGGATTGACGATCCTAAGCACTCGGAATAACTCAAAAGCATCTCCTTTTATCGGCACCTCAAAttttt > 8:3618/4‑139 (MQ=255) gtaTTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTtg > 7:416919/3‑139 (MQ=255) gtaTTAACCGGTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGAGTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTtg < 2:17127/137‑1 (MQ=255) taTTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGATGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTtgt > 1:70713/2‑139 (MQ=255) taTTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGATGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTtg < 2:70713/137‑1 (MQ=255) taTTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGa > 6:94126/2‑77 (MQ=255) taTTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGa < 5:94126/76‑1 (MQ=255) ccGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGataa > 7:313820/2‑135 (MQ=255) ggTTACGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGataaaa < 5:355172/138‑6 (MQ=255) tCCGTAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGataaaa < 8:112803/136‑6 (MQ=255) tCCGGAGAGCTGCCGACTATTTGTCTGTCTAGCAGTTTCATATTATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATAGGCACCTCAAATTTTTGTGATAAAAca > 4:233284/1‑137 (MQ=255) tCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCa > 2:295700/1‑79 (MQ=255) tCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCa < 1:295700/79‑1 (MQ=255) tCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGataaaa > 5:209745/1‑131 (MQ=255) tCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGataaaa > 7:112803/1‑131 (MQ=255) tCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGataaaa < 6:209745/136‑6 (MQ=255) tCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAAcac > 8:201918/1‑137 (MQ=255) tCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAAcac > 2:14417/1‑137 (MQ=255) gCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAg < 4:185731/139‑1 (MQ=255) cTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGataaaa > 1:409057/1‑122 (MQ=255) cTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGataaaa < 2:409057/127‑6 (MQ=255) cTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTgg > 4:192166/1‑135 (MQ=255) cTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGcc < 4:314034/139‑1 (MQ=255) tttCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGcct < 3:125084/137‑2 (MQ=255) tttCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGAGTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGc < 5:61856/139‑1 (MQ=255) ctctGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCtt < 7:365599/138‑1 (MQ=255) tGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGataaaa > 5:444608/1‑106 (MQ=255) tGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGataaaa < 6:444608/111‑6 (MQ=255) tctagcaGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTtata < 8:98427/132‑1 (MQ=255) tctagcaGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTtata > 5:226192/7‑138 (MQ=255) agcaGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTtatatagc > 3:81931/4‑137 (MQ=255) tCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGcc > 4:158538/1‑118 (MQ=255) tCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGcc < 3:158538/118‑1 (MQ=255) ataCATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGACTGCTTTTATATAGCTTTTGATTTTTTc > 5:334050/1‑138 (MQ=255) taCATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCt > 3:304310/1‑138 (MQ=255) taCATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTa > 7:75870/1‑139 (MQ=255) taCATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTa > 3:147970/1‑139 (MQ=255) taCATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTa > 1:238672/1‑139 (MQ=255) taCATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTa > 1:17997/1‑139 (MQ=255) ataCGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACAt > 2:280430/1‑137 (MQ=255) taCGGAGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATcct < 3:233284/139‑3 (MQ=255) | CGCCTCGTTTTCTCGTACCGGCAATCGGATGGATTTCCAAGTGCCCGTCCTTAACGTGGATTAATCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTTGGAGTTTCATATAATACATATACGGAGAAGGATTGACGATCCTAAGCACCCGGTATAACTCAAAGGCATCTGATTTTATCGGAATCTCAAATTTTTGTGACAGAACGCCTTGGAAGATATCGCCCGCTTTTATATATTCTTTTATTTTTTCTACATCAG > CP006881/2194318‑2194575 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |