| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 2,194,466 | C→T | R272R (CGG→CGA) | U712_10995 ← | Anthranilate synthase component 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,194,466 | 0 | C | T | 98.5% | 197.9 / ‑4.5 | 66 | R272R (CGG→CGA) | U712_10995 | Anthranilate synthase component 1 |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (33/32); total (34/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01 | |||||||||||
TACCGGCAATCGGATGGATTTCCAAGTGCCCGTCCTTAACGTGGATTAATCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTTGGAGTTTCATATAATACATATACGGAGAAGGATTGACGATCCTAAGCACCCGGTATAACTCAAAGGCATCTGATTTTATCGGAATCTCAAATTTTTGTGACAGAACGCCTTGGAAGATATCGCCCGCTTTTATATATTCTTTTATTTTTTCTACATCAGCCATAAAGGCTGGTTTTTCATAA > CP006881/2194333‑2194598 | tACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGta < 7:290606/139‑1 (MQ=255)tACCGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGta < 6:41292/139‑1 (MQ=255) ccGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGtat < 3:356414/138‑1 (MQ=255) cGGCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATaa < 2:271697/139‑1 (MQ=255) gCAATCGGATGGATTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACt > 3:314034/1‑139 (MQ=255) tggatTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCAt < 3:211560/139‑1 (MQ=255) tggatTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCAt < 1:279980/139‑1 (MQ=255) tggatTTCTAAGTGCCCGTCTTGAACGTGTATTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCAt < 5:193414/139‑1 (MQ=255) tgtaTTAACCGATCCGGAGAGCTGCCGACTATTTCTATGTATAGCAGTTTCATAATATACATATACGGATAAGGATTGACGATCCTAAGCACTCGGAATAACTCAAAAGCATCTCCTTTTATCGGCACCTCAAAttttt > 8:3618/4‑139 (MQ=255) gtaTTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTtg > 7:416919/3‑139 (MQ=255) gtaTTAACCGGTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGAGTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTtg < 2:17127/137‑1 (MQ=255) taTTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGATGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTtgt > 1:70713/2‑139 (MQ=255) taTTAACCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGATGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTtg < 2:70713/137‑1 (MQ=255) ccGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGataa > 7:313820/2‑135 (MQ=255) ggTTACGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGataaaa < 5:355172/138‑6 (MQ=255) tCCGTAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGataaaa < 8:112803/136‑6 (MQ=255) tCCGGAGAGCTGCCGACTATTTGTCTGTCTAGCAGTTTCATATTATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATAGGCACCTCAAATTTTTGTGATAAAAca > 4:233284/1‑137 (MQ=255) tCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGataaaa < 6:209745/136‑6 (MQ=255) tCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGataaaa > 7:112803/1‑131 (MQ=255) tCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGataaaa > 5:209745/1‑131 (MQ=255) tCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAAcac > 2:14417/1‑137 (MQ=255) tCCGGAGAGCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAAcac > 8:201918/1‑137 (MQ=255) gCTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAg < 4:185731/139‑1 (MQ=255) cTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGataaaa < 2:409057/127‑6 (MQ=255) cTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGataaaa > 1:409057/1‑122 (MQ=255) cTGCCGACTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTgg > 4:192166/1‑135 (MQ=255) cTATTTCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGcc < 4:314034/139‑1 (MQ=255) tttCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGcct < 3:125084/137‑2 (MQ=255) tttCTCTGTCTAGCAGTTTCATATAATACATATACGGCGAAGGAGTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGc < 5:61856/139‑1 (MQ=255) ctctGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCtt < 7:365599/138‑1 (MQ=255) tGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGataaaa > 5:444608/1‑106 (MQ=255) tGTCTAGCAGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGataaaa < 6:444608/111‑6 (MQ=255) tctagcaGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTtata < 8:98427/132‑1 (MQ=255) tctagcaGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTtata > 5:226192/7‑138 (MQ=255) agcaGTTTCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTtatatagc > 3:81931/4‑137 (MQ=255) tCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGcc > 4:158538/1‑118 (MQ=255) tCATATAATACATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGcc < 3:158538/118‑1 (MQ=255) ataCATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGACTGCTTTTATATAGCTTTTGATTTTTTc > 5:334050/1‑138 (MQ=255) taCATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCt > 3:304310/1‑138 (MQ=255) taCATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTa > 7:75870/1‑139 (MQ=255) taCATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTa > 1:238672/1‑139 (MQ=255) taCATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTa > 1:17997/1‑139 (MQ=255) taCATATACGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTa > 3:147970/1‑139 (MQ=255) ataCGGCGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACAt > 2:280430/1‑137 (MQ=255) taCGGAGAAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATcct < 3:233284/139‑3 (MQ=255) gcgaAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCa > 8:168646/3‑138 (MQ=255) gcgaAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTGTTGATTTTTTCTACATCAGCCa > 6:106504/3‑138 (MQ=255) cgaAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATTTAGCTTTTGATTTTTTCTACATCAGCCAt > 8:222843/2‑138 (MQ=255) cgaAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCAt > 1:430501/2‑138 (MQ=255) cgaAGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGGGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCAt > 3:239005/2‑138 (MQ=255) aaGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTtatatagctt > 3:223056/1‑109 (MQ=255) aaGGATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTtatatagctt < 4:223056/113‑5 (MQ=255) ggATTGACGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATaaaag > 7:200590/1‑137 (MQ=255) aCGATCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGccgatt > 3:354680/1‑134 (MQ=255) aTCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGAttttt > 7:241328/1‑139 (MQ=255) ccTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCa > 3:212807/1‑139 (MQ=255) ccTAAGCACCCGGTATAACTCAAAGGCATCTGATTTTATCGGAATCTCAAATTTTTGTGACAGAACGCCTTGGAAGATATCGCCCGCTTTTATATATTCTTTTATTTTTTCTACATCAGCCATAAAGGCTGGTTTTTCa > 5:307581/1‑139 (MQ=255) cTAAGCTCTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCATATTTTTGTGATAAAACACCCTGGAAGATATCGCCTTCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCAt < 1:413926/139‑1 (MQ=255) cTAAGCACTCGGTATAACTCAGAAGCATCTGCTTTATTCGGCACCTCAAAATTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCa < 4:238892/138‑1 (MQ=255) cTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCAt < 7:3618/139‑1 (MQ=255) cTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCAt < 1:280430/139‑1 (MQ=255) aGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATaa < 3:192166/138‑1 (MQ=255) aGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATaa < 5:95098/138‑1 (MQ=255) aGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATaa < 4:282304/138‑1 (MQ=255) aGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATaa < 8:75870/138‑1 (MQ=255) aGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTGTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATaa < 4:288171/138‑1 (MQ=255) | TACCGGCAATCGGATGGATTTCCAAGTGCCCGTCCTTAACGTGGATTAATCGTTCCGGAGAGCTGCCGACTATTTCTCTGTCTTGGAGTTTCATATAATACATATACGGAGAAGGATTGACGATCCTAAGCACCCGGTATAACTCAAAGGCATCTGATTTTATCGGAATCTCAAATTTTTGTGACAGAACGCCTTGGAAGATATCGCCCGCTTTTATATATTCTTTTATTTTTTCTACATCAGCCATAAAGGCTGGTTTTTCATAA > CP006881/2194333‑2194598 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |