Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,194,586 T→C P232S (CCA→TCG)  U712_10995 ← Anthranilate synthase component 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,194,5860TC98.9% 283.2 / ‑4.8 90P232S (CCA→TCG) U712_10995Anthranilate synthase component 1
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (48/41);  total (49/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01

ATCCTAAGCACCCGGTATAACTCAAAGGCATCTGATTTTATCGGAATCTCAAATTTTTGTGACAGAACGCCTTGGAAGATATCGCCCGCTTTTATATATTCTTTTATTTTTTCTACATCAGCCATAAAGGCTGGTTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTATATGAATCAGCAGAAAGAAACAGTTCTTTTATGTTTTTTTGATCCATCATTTTTTCTATGAGATCTTGAAGCTCTTGATGATTT  >  CP006881/2194455‑2194715
                                                                                                                                   |                                                                                                                                 
aTCCTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGAttttt                                                                                                                            >  7:241328/1‑139 (MQ=255)
  ccTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCa                                                                                                                          >  3:212807/1‑139 (MQ=255)
  ccTAAGCACCCGGTATAACTCAAAGGCATCTGATTTTATCGGAATCTCAAATTTTTGTGACAGAACGCCTTGGAAGATATCGCCCGCTTTTATATATTCTTTTATTTTTTCTACATCAGCCATAAAGGCTGGTTTTTCa                                                                                                                          >  5:307581/1‑139 (MQ=255)
   cTAAGCTCTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCATATTTTTGTGATAAAACACCCTGGAAGATATCGCCTTCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCAt                                                                                                                         <  1:413926/139‑1 (MQ=255)
   cTAAGCACTCGGTATAACTCAGAAGCATCTGCTTTATTCGGCACCTCAAAATTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCa                                                                                                                          <  4:238892/138‑1 (MQ=255)
   cTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCAt                                                                                                                         <  1:280430/139‑1 (MQ=255)
   cTAAGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCAt                                                                                                                         <  7:3618/139‑1 (MQ=255)
      aGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATaa                                                                                                                       <  8:75870/138‑1 (MQ=255)
      aGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATaa                                                                                                                       <  4:282304/138‑1 (MQ=255)
      aGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATaa                                                                                                                       <  5:95098/138‑1 (MQ=255)
      aGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATaa                                                                                                                       <  3:192166/138‑1 (MQ=255)
      aGCACTCGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTGTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATaa                                                                                                                       <  4:288171/138‑1 (MQ=255)
          ctcGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTaga                                                                                                                  <  2:75118/137‑1 (MQ=255)
          ctcGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTaga                                                                                                                  <  8:200590/137‑1 (MQ=255)
           tcGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTag                                                                                                                   >  4:184063/2‑137 (MQ=255)
           tcGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTag                                                                                                                   >  8:369205/2‑137 (MQ=255)
           tcGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTag                                                                                                                   >  4:65584/2‑137 (MQ=255)
           tcGGTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGACTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTag                                                                                                                   >  6:184858/2‑137 (MQ=255)
             ggTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTagaaga                                                                                                               <  4:304310/139‑1 (MQ=255)
             ggTATAACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTag                                                                                                                   >  3:60680/1‑135 (MQ=255)
               tataACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATa                                                                                                             >  4:277760/1‑139 (MQ=255)
                 taACTGAAAATCATCTTCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACGCTGGAAGATATCGACTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATAc                                                                                                            <  8:421299/138‑1 (MQ=255)
                 taACTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATAc                                                                                                            <  7:168646/138‑1 (MQ=255)
                    cTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGtc                                                                                                        >  8:366384/1‑139 (MQ=255)
                    cTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGtc                                                                                                        >  6:431863/1‑139 (MQ=255)
                    cTCAAAAGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGtc                                                                                                        >  8:314623/1‑139 (MQ=255)
                       aaaaGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTGTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCa                                                                                                     <  4:334737/139‑1 (MQ=255)
                       aaaaGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGtctc                                                                                                      >  2:354099/1‑138 (MQ=255)
                       aaaaGCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGtct                                                                                                       <  8:313820/137‑1 (MQ=255)
                         aagCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCTCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCa                                                                                                     >  8:109156/3‑137 (MQ=255)
                         aagCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCaa                                                                                                    >  4:6844/3‑138 (MQ=255)
                         aagCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCa                                                                                                     >  6:436299/3‑137 (MQ=255)
                         aagCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCa                                                                                                     >  3:133300/3‑137 (MQ=255)
                         aagCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCa                                                                                                     >  1:19554/3‑137 (MQ=255)
                         aagCATCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAAAACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGACTCa                                                                                                     >  7:421299/3‑137 (MQ=255)
                             aTCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTg                                                                                               <  6:226192/139‑1 (MQ=255)
                              tCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGc                                                                                                 <  3:405829/136‑1 (MQ=255)
                              tCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGc                                                                                                 >  4:405829/1‑136 (MQ=255)
                              tCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTgg                                                                                              >  6:404018/1‑139 (MQ=255)
                              tCTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTgg                                                                                              >  2:244220/1‑139 (MQ=255)
                               cTGCTTTTATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTggg                                                                                             >  3:225804/1‑139 (MQ=255)
                                  cttttATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTgg                                                                                              <  4:225804/134‑1 (MQ=255)
                                  cttttATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTgg                                                                                              <  6:429517/134‑1 (MQ=255)
                                  cttttATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGgtg                                                                                           <  1:244220/137‑1 (MQ=255)
                                     ttATCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTc                                                                                         <  8:241328/137‑1 (MQ=255)
                                       aTCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATAc                                                                                                            <  3:146482/116‑1 (MQ=255)
                                       aTCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATAc                                                                                                            >  4:146482/1‑116 (MQ=255)
                                       aTCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCttg                                                                                      >  3:164531/1‑137 (MQ=255)
                                       aTCGGCACCTCAAATTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCttg                                                                                      >  1:111982/1‑137 (MQ=255)
                                                    aTTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCaga                                                                        <  2:430501/139‑1 (MQ=255)
                                                    aTTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCaga                                                                        <  5:184858/139‑1 (MQ=255)
                                                    aTTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCaga                                                                        <  3:184063/139‑1 (MQ=255)
                                                     tttttGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCagaa                                                                       <  6:334050/139‑1 (MQ=255)
                                                                      cctGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTAt                                                      >  4:74546/3‑139 (MQ=255)
                                                                      cctGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTAt                                                      >  4:46234/3‑139 (MQ=255)
                                                                        tGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCTGCAGAAAGAAAGAGCTCTTTTATGt                                                    >  2:107268/1‑139 (MQ=255)
                                                                        tGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGt                                                    >  7:179542/1‑139 (MQ=255)
                                                                          gAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTTTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGt                                                    <  3:6844/137‑1 (MQ=255)
                                                                                atCGCCTGGTTTTATATAGCTTTTGATGTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGGAACAGCTCTTTTTTGGTttgttgg                                            >  6:13803/1‑138 (MQ=255)
                                                                                atCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCa                                                                                                     <  1:154020/82‑1 (MQ=255)
                                                                                atCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTATCa                                                                                                     >  2:154020/1‑82 (MQ=255)
                                                                                  cGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGAttttt                                                                                                                            <  7:192769/57‑1 (MQ=16)
                                                                                  cGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGAttttt                                                                                                                            >  8:192769/1‑57 (MQ=16)
                                                                                    cctgCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCca                                        >  5:300157/4‑139 (MQ=255)
                                                                                    cctgCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCca                                        >  1:47849/4‑139 (MQ=255)
                                                                                    cctgCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCca                                        >  7:295377/4‑139 (MQ=255)
                                                                                        cTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTgg                                                                                              >  8:94719/1‑81 (MQ=255)
                                                                                        cTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTgg                                                                                              <  7:94719/81‑1 (MQ=255)
                                                                                                  gcttttgATTTTTTCTACATCAGCGATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAAt                           >  1:179382/4‑138 (MQ=255)
                                                                                                  gcttttgATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAAt                           >  2:318354/4‑138 (MQ=255)
                                                                                                  gcttttgATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATg                          <  2:238672/136‑1 (MQ=255)
                                                                                                  gcttttgATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATg                          <  7:201918/136‑1 (MQ=255)
                                                                                                  gcttttgATTTTTTCTACATCAGCCATAAAAGACTATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATg                          <  7:369205/136‑1 (MQ=255)
                                                                                                   cttttgATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTc                                                                                         >  4:446779/3‑75 (MQ=255)
                                                                                                   cttttgATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTc                                                                                         <  3:446779/73‑1 (MQ=255)
                                                                                                   cttttgATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAAt                           >  3:427611/3‑137 (MQ=255)
                                                                                                   cttttgATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAAt                           >  4:248635/3‑137 (MQ=255)
                                                                                                   cttttgATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATga                         >  1:248665/3‑139 (MQ=255)
                                                                                                   cttttgATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATga                         <  5:404018/137‑1 (MQ=255)
                                                                                                     tttgATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAAt                           >  3:392735/1‑135 (MQ=255)
                                                                                                      ttgaTTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGAt                      >  3:445617/4‑139 (MQ=255)
                                                                                                            ttttCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGa                 <  8:179542/138‑1 (MQ=255)
                                                                                                            ttttCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGa                 <  5:431863/138‑1 (MQ=255)
                                                                                                             tttCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCTATGAGATTTTGAAg               >  1:327908/1‑139 (MQ=255)
                                                                                                             tttCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAg               >  2:150761/1‑139 (MQ=255)
                                                                                                              ttCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAg               <  7:109156/138‑1 (MQ=255)
                                                                                                              ttCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGc              >  8:236612/1‑139 (MQ=255)
                                                                                                                      cAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTcaa                            <  6:117271/117‑3 (MQ=255)
                                                                                                                      cAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTcaa                            >  5:117271/1‑115 (MQ=255)
                                                                                                                          cATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAACAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGAttt  <  7:222843/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                 
ATCCTAAGCACCCGGTATAACTCAAAGGCATCTGATTTTATCGGAATCTCAAATTTTTGTGACAGAACGCCTTGGAAGATATCGCCCGCTTTTATATATTCTTTTATTTTTTCTACATCAGCCATAAAGGCTGGTTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTATATGAATCAGCAGAAAGAAACAGTTCTTTTATGTTTTTTTGATCCATCATTTTTTCTATGAGATCTTGAAGCTCTTGATGATTT  >  CP006881/2194455‑2194715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑