Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,194,631	A→G	Y217Y (TAT→TAC)	U712_10995 ←	Anthranilate synthase component 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,194,631	0	A	G	98.1%	163.7 / ‑4.5	53	Y217Y (TAT→TAC)	U712_10995	Anthranilate synthase component 1
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (29/23);  total (29/24)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

ATTTTTGTGACAGAACGCCTTGGAAGATATCGCCCGCTTTTATATATTCTTTTATTTTTTCTACATCAGCCATAAAGGCTGGTTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTATATGAATCAGCAGAAAGAAACAGTTCTTTTATGTTTTTTTGATCCATCATTTTTTCTATGAGATCTTGAAGCTCTTGATGATTTTGATGAAATACATCGATTTTTTC‑GTTTTCTGTTTCCTGTCCATTGAGCCTT  >  CP006881/2194507‑2194766                                                                                                                             |                                                                                                                                         aTTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCaga                                                                                                                            <  5:184858/139‑1 (MQ=255) aTTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCaga                                                                                                                            <  2:430501/139‑1 (MQ=255) aTTTTTGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCaga                                                                                                                            <  3:184063/139‑1 (MQ=255)  tttttGTGATAAAACACCCTGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCagaa                                                                                                                           <  6:334050/139‑1 (MQ=255)                   cctGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTAt                                                                                                          >  4:74546/3‑139 (MQ=255)                   cctGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTAt                                                                                                          >  4:46234/3‑139 (MQ=255)                     tGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCTGCAGAAAGAAAGAGCTCTTTTATGt                                                                                                        >  2:107268/1‑139 (MQ=255)                     tGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGt                                                                                                        >  7:179542/1‑139 (MQ=255)                       gAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTTTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGt                                                                                                        <  3:6844/137‑1 (MQ=255)                             atCGCCTGGTTTTATATAGCTTTTGATGTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGGAACAGCTCTTTTTTGGTttgttgg                                                                                                >  6:13803/1‑138 (MQ=255)                                 cctgCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCca                                                                                            >  5:300157/4‑139 (MQ=255)                                 cctgCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCca                                                                                            >  1:47849/4‑139 (MQ=255)                                 cctgCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCca                                                                                            >  7:295377/4‑139 (MQ=255)                                               gcttttgATTTTTTCTACATCAGCGATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAAt                                                                               >  1:179382/4‑138 (MQ=255)                                               gcttttgATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAAt                                                                               >  2:318354/4‑138 (MQ=255)                                               gcttttgATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATg                                                                              <  7:201918/136‑1 (MQ=255)                                               gcttttgATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATg                                                                              <  2:238672/136‑1 (MQ=255)                                               gcttttgATTTTTTCTACATCAGCCATAAAAGACTATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATg                                                                              <  7:369205/136‑1 (MQ=255)                                                cttttgATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAAt                                                                               >  3:427611/3‑137 (MQ=255)                                                cttttgATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAAt                                                                               >  4:248635/3‑137 (MQ=255)                                                cttttgATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATga                                                                             >  1:248665/3‑139 (MQ=255)                                                cttttgATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATga                                                                             <  5:404018/137‑1 (MQ=255)                                                  tttgATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAAt                                                                               >  3:392735/1‑135 (MQ=255)                                                   ttgaTTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGAt                                                                          >  3:445617/4‑139 (MQ=255)                                                         ttttCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGa                                                                     <  8:179542/138‑1 (MQ=255)                                                         ttttCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGa                                                                     <  5:431863/138‑1 (MQ=255)                                                          tttCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCTATGAGATTTTGAAg                                                                   >  1:327908/1‑139 (MQ=255)                                                          tttCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAg                                                                   >  2:150761/1‑139 (MQ=255)                                                           ttCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAg                                                                   <  7:109156/138‑1 (MQ=255)                                                           ttCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGc                                                                  >  8:236612/1‑139 (MQ=255)                                                                   cAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTcaa                                                                                >  5:117271/1‑115 (MQ=255)                                                                   cAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTcaa                                                                                <  6:117271/117‑3 (MQ=255)                                                                       cATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAACAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGAttt                                                      <  7:222843/139‑1 (MQ=255)                                                                               ccgatttttCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGgaa                                              <  4:392735/135‑1 (MQ=255)                                                                               ccgatttttCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGgaa                                              <  1:354099/135‑1 (MQ=255)                                                                                                     tACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGTTTTTGAAGATACAGATGATTTTGATGGAATACATCCATTTTTTCTGttttt                        >  4:267897/1‑138 (MQ=255)                                                                                                      aCTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTg                      >  4:287536/1‑139 (MQ=255)                                                                                                       cTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGt                     <  8:295377/139‑1 (MQ=255)                                                                                                       cTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGt                     <  3:65584/139‑1 (MQ=255)                                                                                                       cTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGt                     <  2:47849/139‑1 (MQ=255)                                                                                                        tGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGtt                    >  5:119233/1‑139 (MQ=255)                                                                                                        tGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGtt                    >  1:434897/1‑139 (MQ=255)                                                                                                          tctcAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGttt                   <  4:354680/138‑1 (MQ=255)                                                                                                          tctcAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGttt                   >  3:303000/1‑138 (MQ=255)                                                                                                              aaaGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGTTTCCt                <  3:287536/137‑1 (MQ=255)                                                                                                               aaGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTtga                                                   >  6:46151/1‑102 (MQ=255)                                                                                                               aaGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTtga                                                   <  5:46151/102‑1 (MQ=255)                                                                                                                    gggTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGTTTCATCTCCagt         >  2:245082/1‑136 (MQ=255)                                                                                                                     ggTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGTTTCCTCTCCagt         <  4:81931/137‑3 (MQ=255)                                                                                                                     ggTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGTTTCCTCTCCagt         >  8:317002/1‑135 (MQ=255)                                                                                                                     ggTGTCTTATATGAATCAGCAGAAAGAAACAGTTCTTTTATGTTTTTTTGATCCATCATTTTTTCTATGAGATCTTGAAGCTCTTGATGATTTTGATGAAATACATCGATTTTTTC‑GTTTTCTGTTTCCTGTCCAtt         <  6:307581/137‑1 (MQ=255)                                                                                                                        gtCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGTTTCCTCTCCAGTGAGcc    >  4:158631/1‑139 (MQ=255)                                                                                                                          cTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGTTTCCTCTCCAGTGAGCCtt  >  1:105018/1‑139 (MQ=255)                                                                                                                             |                                                                                                                                         ATTTTTGTGACAGAACGCCTTGGAAGATATCGCCCGCTTTTATATATTCTTTTATTTTTTCTACATCAGCCATAAAGGCTGGTTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTATATGAATCAGCAGAAAGAAACAGTTCTTTTATGTTTTTTTGATCCATCATTTTTTCTATGAGATCTTGAAGCTCTTGATGATTTTGATGAAATACATCGATTTTTTC‑GTTTTCTGTTTCCTGTCCATTGAGCCTT  >  CP006881/2194507‑2194766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑